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The C. elegans TPR Containing Protein, TRD-1, Regulates Cell Fate Choice in the Developing Germ Line and Epidermis.

Hughes S, Wilkinson H, Gilbert SP, Kishida M, Ding SS, Woollard A - PLoS ONE (2014)

Bottom Line: In the germline, stem cells adopt one of three possible fates: mitotic cell cycle, or gamete formation via meiosis, producing either sperm or oocytes.In the epidermis, the stem cell-like seam cells divide asymmetrically, with the daughters taking on either a proliferative (seam) or differentiated (hypodermal or neuronal) fate.We show that trd-1(RNAi) and mutant animals have fewer seam cells as a result of inappropriate differentiation towards the hypodermal fate.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Oxford, Oxford, United Kingdom.

ABSTRACT
Correct cell fate choice is crucial in development. In post-embryonic development of the hermaphroditic Caenorhabitis elegans, distinct cell fates must be adopted in two diverse tissues. In the germline, stem cells adopt one of three possible fates: mitotic cell cycle, or gamete formation via meiosis, producing either sperm or oocytes. In the epidermis, the stem cell-like seam cells divide asymmetrically, with the daughters taking on either a proliferative (seam) or differentiated (hypodermal or neuronal) fate. We have isolated a novel conserved C. elegans tetratricopeptide repeat containing protein, TRD-1, which is essential for cell fate determination in both the germline and the developing epidermis and has homologs in other species, including humans (TTC27). We show that trd-1(RNAi) and mutant animals have fewer seam cells as a result of inappropriate differentiation towards the hypodermal fate. In the germline, trd-1 RNAi results in a strong masculinization phenotype, as well as defects in the mitosis to meiosis switch. Our data suggests that trd-1 acts downstream of tra-2 but upstream of fem-3 in the germline sex determination pathway, and exhibits a constellation of phenotypes in common with other Mog (masculinization of germline) mutants. Thus, trd-1 is a new player in both the somatic and germline cell fate determination machinery, suggestive of a novel molecular connection between the development of these two diverse tissues.

No MeSH data available.


Related in: MedlinePlus

Localization of TRD-1.(A) Strong cytoplasmic expression of trd-1::gfp (strain, NK624) was observed in seam cells throughout postembryonic development. (B) There is significant expression of trd-1::gfp in the distal tip cell (DTC) at L4. (C) Strong expression of trd-1::gfp is observed in the spermatheca in young adults. Open circles indicate an unfertilized oocyte and closed circles are fertilized eggs. In all cases, Anterior is to the left, ventral bottom. Scale bars are 20 µm.
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pone-0114998-g003: Localization of TRD-1.(A) Strong cytoplasmic expression of trd-1::gfp (strain, NK624) was observed in seam cells throughout postembryonic development. (B) There is significant expression of trd-1::gfp in the distal tip cell (DTC) at L4. (C) Strong expression of trd-1::gfp is observed in the spermatheca in young adults. Open circles indicate an unfertilized oocyte and closed circles are fertilized eggs. In all cases, Anterior is to the left, ventral bottom. Scale bars are 20 µm.

Mentions: Using an integrated GFP reporter (strain NK624, a kind gift from David Sherwood [28]), we observed the expression pattern of trd-1. Fluorescence microscopy of trd-1::gfp shows a strong expression pattern of trd-1 in the cytoplasm of seam cells (Fig. 3A). In addition, trd-1 expression was observed in the developing gonad, including strong expression in the DTC (Figure 3B) and in the spermatheca from the late L3 stage onwards (Fig. 3C). Additionally, we observed expression of trd-1::gfp in the Pn.p cells and anchor cell, as previously reported [28].


The C. elegans TPR Containing Protein, TRD-1, Regulates Cell Fate Choice in the Developing Germ Line and Epidermis.

Hughes S, Wilkinson H, Gilbert SP, Kishida M, Ding SS, Woollard A - PLoS ONE (2014)

Localization of TRD-1.(A) Strong cytoplasmic expression of trd-1::gfp (strain, NK624) was observed in seam cells throughout postembryonic development. (B) There is significant expression of trd-1::gfp in the distal tip cell (DTC) at L4. (C) Strong expression of trd-1::gfp is observed in the spermatheca in young adults. Open circles indicate an unfertilized oocyte and closed circles are fertilized eggs. In all cases, Anterior is to the left, ventral bottom. Scale bars are 20 µm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262444&req=5

pone-0114998-g003: Localization of TRD-1.(A) Strong cytoplasmic expression of trd-1::gfp (strain, NK624) was observed in seam cells throughout postembryonic development. (B) There is significant expression of trd-1::gfp in the distal tip cell (DTC) at L4. (C) Strong expression of trd-1::gfp is observed in the spermatheca in young adults. Open circles indicate an unfertilized oocyte and closed circles are fertilized eggs. In all cases, Anterior is to the left, ventral bottom. Scale bars are 20 µm.
Mentions: Using an integrated GFP reporter (strain NK624, a kind gift from David Sherwood [28]), we observed the expression pattern of trd-1. Fluorescence microscopy of trd-1::gfp shows a strong expression pattern of trd-1 in the cytoplasm of seam cells (Fig. 3A). In addition, trd-1 expression was observed in the developing gonad, including strong expression in the DTC (Figure 3B) and in the spermatheca from the late L3 stage onwards (Fig. 3C). Additionally, we observed expression of trd-1::gfp in the Pn.p cells and anchor cell, as previously reported [28].

Bottom Line: In the germline, stem cells adopt one of three possible fates: mitotic cell cycle, or gamete formation via meiosis, producing either sperm or oocytes.In the epidermis, the stem cell-like seam cells divide asymmetrically, with the daughters taking on either a proliferative (seam) or differentiated (hypodermal or neuronal) fate.We show that trd-1(RNAi) and mutant animals have fewer seam cells as a result of inappropriate differentiation towards the hypodermal fate.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Oxford, Oxford, United Kingdom.

ABSTRACT
Correct cell fate choice is crucial in development. In post-embryonic development of the hermaphroditic Caenorhabitis elegans, distinct cell fates must be adopted in two diverse tissues. In the germline, stem cells adopt one of three possible fates: mitotic cell cycle, or gamete formation via meiosis, producing either sperm or oocytes. In the epidermis, the stem cell-like seam cells divide asymmetrically, with the daughters taking on either a proliferative (seam) or differentiated (hypodermal or neuronal) fate. We have isolated a novel conserved C. elegans tetratricopeptide repeat containing protein, TRD-1, which is essential for cell fate determination in both the germline and the developing epidermis and has homologs in other species, including humans (TTC27). We show that trd-1(RNAi) and mutant animals have fewer seam cells as a result of inappropriate differentiation towards the hypodermal fate. In the germline, trd-1 RNAi results in a strong masculinization phenotype, as well as defects in the mitosis to meiosis switch. Our data suggests that trd-1 acts downstream of tra-2 but upstream of fem-3 in the germline sex determination pathway, and exhibits a constellation of phenotypes in common with other Mog (masculinization of germline) mutants. Thus, trd-1 is a new player in both the somatic and germline cell fate determination machinery, suggestive of a novel molecular connection between the development of these two diverse tissues.

No MeSH data available.


Related in: MedlinePlus