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Exercise Increases Markers of Spermatogenesis in Rats Selectively Bred for Low Running Capacity.

Torma F, Koltai E, Nagy E, Ziaaldini MM, Posa A, Koch LG, Britton SL, Boldogh I, Radak Z - PLoS ONE (2014)

Bottom Line: Twelve weeks of training significantly increased maximal oxygen consumption in LCR by nearly 40% whereas HCR remained unchanged.LCR-T had significantly higher levels of peroxisome proliferator-activated receptor-gamma coactivator-1 (PGC-1α), decreased levels of reactive oxygen species and increased acetylated p53 compared to LCR-C, while training produced no significant changes for these measures in HCR rats.The levels of outer dense fibers -1 (Odf-1), a marker of spermatogenesis, increased in LCR-T rats, but decreased in HCR-TR rats.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.

ABSTRACT
The oxidative stress effect of exercise training on testis function is under debate. In the present study we used a unique rat model system developed by artificial selection for low and high intrinsic running capacity (LCR and HCR, respectively) to evaluate the effects of exercise training on apoptosis and spermatogenesis in testis. Twenty-four 13-month-old male rats were assigned to four groups: control LCR (LCR-C), trained LCR (LCR-T), control HCR (HCR-C), and trained HCR (HCR-T). Ten key proteins connecting aerobic exercise capacity and general testes function were assessed, including those that are vital for mitochondrial biogenesis. The VO2 max of LCR-C group was about 30% lower than that of HCR-C rats, and the SIRT1 levels were also significantly lower than HCR-C. Twelve weeks of training significantly increased maximal oxygen consumption in LCR by nearly 40% whereas HCR remained unchanged. LCR-T had significantly higher levels of peroxisome proliferator-activated receptor-gamma coactivator-1 (PGC-1α), decreased levels of reactive oxygen species and increased acetylated p53 compared to LCR-C, while training produced no significant changes for these measures in HCR rats. BAX and Blc-2 were not different among all four groups. The levels of outer dense fibers -1 (Odf-1), a marker of spermatogenesis, increased in LCR-T rats, but decreased in HCR-TR rats. Moreover, exercise training increased the levels of lactate dehydrogenase C (LDHC) only in LCR rats. These data suggest that rats with low inborn exercise capacity can increase whole body oxygen consumption and running exercise capacity with endurance training and, in turn, increase spermatogenesis function via reduction in ROS and heightened activity of p53 in testes.

No MeSH data available.


Related in: MedlinePlus

Oxidative stress markers.Rat gastrocnemius muscle was stained with dichlorodihydrofluoresceindiacetate (H2DCFDA) to measure relative steady-state oxidant levels and redox-active iron release levels (both increase DCF fluorescence) as an estimate of levels of reactive oxygen species (ROS).Control LCR (LCR-C), trained LCR (LCR-T), high capacity control (HCR-C) and trained (HCR-T) groups. Values are means ± SD for six animals per group *Significantly different from control group, +Significantly different from trained group, p<0.05.
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pone-0114075-g002: Oxidative stress markers.Rat gastrocnemius muscle was stained with dichlorodihydrofluoresceindiacetate (H2DCFDA) to measure relative steady-state oxidant levels and redox-active iron release levels (both increase DCF fluorescence) as an estimate of levels of reactive oxygen species (ROS).Control LCR (LCR-C), trained LCR (LCR-T), high capacity control (HCR-C) and trained (HCR-T) groups. Values are means ± SD for six animals per group *Significantly different from control group, +Significantly different from trained group, p<0.05.

Mentions: The level of testicular ROS was appraised by fluorescence intensity of H2DCFDA and exercise training decreased the level of ROS only in LCR-T rats compared to HCR-T group (Fig. 2). SIRT1 levels were significantly higher in HCR-C group compared to the LCR-C group and training decreased the protein content of HCR rats (Fig. 3A).Since SIRT1 can deacetylate PGC-1α, we checked the protein content of this transcriptional co-activator and found PGC-1α increased only in the LCR-T group (p<0.05, Fig. 3B). The levels of NRF1 were not different for any of the groups (Fig. 3C). On the other hand, testicular mtTFA levels were decreased only in the HCR-T group (Fig. 3D).


Exercise Increases Markers of Spermatogenesis in Rats Selectively Bred for Low Running Capacity.

Torma F, Koltai E, Nagy E, Ziaaldini MM, Posa A, Koch LG, Britton SL, Boldogh I, Radak Z - PLoS ONE (2014)

Oxidative stress markers.Rat gastrocnemius muscle was stained with dichlorodihydrofluoresceindiacetate (H2DCFDA) to measure relative steady-state oxidant levels and redox-active iron release levels (both increase DCF fluorescence) as an estimate of levels of reactive oxygen species (ROS).Control LCR (LCR-C), trained LCR (LCR-T), high capacity control (HCR-C) and trained (HCR-T) groups. Values are means ± SD for six animals per group *Significantly different from control group, +Significantly different from trained group, p<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262201&req=5

pone-0114075-g002: Oxidative stress markers.Rat gastrocnemius muscle was stained with dichlorodihydrofluoresceindiacetate (H2DCFDA) to measure relative steady-state oxidant levels and redox-active iron release levels (both increase DCF fluorescence) as an estimate of levels of reactive oxygen species (ROS).Control LCR (LCR-C), trained LCR (LCR-T), high capacity control (HCR-C) and trained (HCR-T) groups. Values are means ± SD for six animals per group *Significantly different from control group, +Significantly different from trained group, p<0.05.
Mentions: The level of testicular ROS was appraised by fluorescence intensity of H2DCFDA and exercise training decreased the level of ROS only in LCR-T rats compared to HCR-T group (Fig. 2). SIRT1 levels were significantly higher in HCR-C group compared to the LCR-C group and training decreased the protein content of HCR rats (Fig. 3A).Since SIRT1 can deacetylate PGC-1α, we checked the protein content of this transcriptional co-activator and found PGC-1α increased only in the LCR-T group (p<0.05, Fig. 3B). The levels of NRF1 were not different for any of the groups (Fig. 3C). On the other hand, testicular mtTFA levels were decreased only in the HCR-T group (Fig. 3D).

Bottom Line: Twelve weeks of training significantly increased maximal oxygen consumption in LCR by nearly 40% whereas HCR remained unchanged.LCR-T had significantly higher levels of peroxisome proliferator-activated receptor-gamma coactivator-1 (PGC-1α), decreased levels of reactive oxygen species and increased acetylated p53 compared to LCR-C, while training produced no significant changes for these measures in HCR rats.The levels of outer dense fibers -1 (Odf-1), a marker of spermatogenesis, increased in LCR-T rats, but decreased in HCR-TR rats.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.

ABSTRACT
The oxidative stress effect of exercise training on testis function is under debate. In the present study we used a unique rat model system developed by artificial selection for low and high intrinsic running capacity (LCR and HCR, respectively) to evaluate the effects of exercise training on apoptosis and spermatogenesis in testis. Twenty-four 13-month-old male rats were assigned to four groups: control LCR (LCR-C), trained LCR (LCR-T), control HCR (HCR-C), and trained HCR (HCR-T). Ten key proteins connecting aerobic exercise capacity and general testes function were assessed, including those that are vital for mitochondrial biogenesis. The VO2 max of LCR-C group was about 30% lower than that of HCR-C rats, and the SIRT1 levels were also significantly lower than HCR-C. Twelve weeks of training significantly increased maximal oxygen consumption in LCR by nearly 40% whereas HCR remained unchanged. LCR-T had significantly higher levels of peroxisome proliferator-activated receptor-gamma coactivator-1 (PGC-1α), decreased levels of reactive oxygen species and increased acetylated p53 compared to LCR-C, while training produced no significant changes for these measures in HCR rats. BAX and Blc-2 were not different among all four groups. The levels of outer dense fibers -1 (Odf-1), a marker of spermatogenesis, increased in LCR-T rats, but decreased in HCR-TR rats. Moreover, exercise training increased the levels of lactate dehydrogenase C (LDHC) only in LCR rats. These data suggest that rats with low inborn exercise capacity can increase whole body oxygen consumption and running exercise capacity with endurance training and, in turn, increase spermatogenesis function via reduction in ROS and heightened activity of p53 in testes.

No MeSH data available.


Related in: MedlinePlus