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Increased expression of AQP 1 and AQP 5 in rat lungs ventilated with low tidal volume is time dependent.

Fabregat G, García-de-la-Asunción J, Sarriá B, Cortijo J, De Andrés J, Mata M, Pastor E, Belda FJ - PLoS ONE (2014)

Bottom Line: Lung oedema and alveolar-capillary membrane permeability did not change during MV.But in AQP-1 expression these differences were not found.AQPs may have a protective effect against the oedema induced by MV.

View Article: PubMed Central - PubMed

Affiliation: Hospital General Universitario, Valencia, Spain.

ABSTRACT

Background and goals: Mechanical ventilation (MV) can induce or worsen pulmonary oedema. Aquaporins (AQPs) facilitate the selective and rapid bi-directional movement of water. Their role in the development and resolution of pulmonary oedema is controversial. Our objectives are to determine if prolonged MV causes lung oedema and changes in the expression of AQP 1 and AQP 5 in rats.

Methods: 25 male Wistar rats were subjected to MV with a tidal volume of 10 ml/kg, during 2 hours (n = 12) and 4 hours (n = 13). Degree of oedema was compared with a group of non-ventilated rats (n = 5). The expression of AQP 1 and AQP 5 were determined by western immunoblotting, measuring the amount of mRNA (previously amplified by RT-PCR) and immunohistochemical staining of AQPs 1 and 5 in lung samples from all groups.

Results: Lung oedema and alveolar-capillary membrane permeability did not change during MV. AQP-5 steady state levels in the western blot were increased (p<0.01) at 2 h and 4 h of MV. But in AQP-1 expression these differences were not found. However, the amount of mRNA for AQP-1 was increased at 2 h and 4 h of MV; and for AQP 5 at 4 h of MV. These findings were corroborated by representative immunohistochemical lung samples.

Conclusion: In lungs from rats ventilated with a low tidal volume the expression of AQP 5 increases gradually with MV duration, but does not cause pulmonary oedema or changes in lung permeability. AQPs may have a protective effect against the oedema induced by MV.

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Related in: MedlinePlus

Dispersion chart and regression lines for Western Blot densitometries, AQP 5, cytosolic and membrane, in relation to time.Values correspond to dispersion coefficients R2. AQP 5 cyt.  =  Aquaporin 5, cytosolic; AQP 5 mb.  =  Aquaporin 5, membrane.
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pone-0114247-g008: Dispersion chart and regression lines for Western Blot densitometries, AQP 5, cytosolic and membrane, in relation to time.Values correspond to dispersion coefficients R2. AQP 5 cyt.  =  Aquaporin 5, cytosolic; AQP 5 mb.  =  Aquaporin 5, membrane.

Mentions: AQP-5 steady state levels in cytoplasm and membranes was significantly greater in both groups (2H and 4H) vs. the controls. Besides, AQP-5 expression on cytoplasm and membranes was greater in group 4H than in group 2H (p = 0.027 and p = 0.039, respectively) (Figs. 5 and 7). To better characterize these differences in the expression of AQP 5, a regression analysis of both variables was conducted, which provided a coefficient of determination R2 = 0.80 (p = 0.008) and R2 = 0.90 (p = 0.001) (Fig. 8).


Increased expression of AQP 1 and AQP 5 in rat lungs ventilated with low tidal volume is time dependent.

Fabregat G, García-de-la-Asunción J, Sarriá B, Cortijo J, De Andrés J, Mata M, Pastor E, Belda FJ - PLoS ONE (2014)

Dispersion chart and regression lines for Western Blot densitometries, AQP 5, cytosolic and membrane, in relation to time.Values correspond to dispersion coefficients R2. AQP 5 cyt.  =  Aquaporin 5, cytosolic; AQP 5 mb.  =  Aquaporin 5, membrane.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4260863&req=5

pone-0114247-g008: Dispersion chart and regression lines for Western Blot densitometries, AQP 5, cytosolic and membrane, in relation to time.Values correspond to dispersion coefficients R2. AQP 5 cyt.  =  Aquaporin 5, cytosolic; AQP 5 mb.  =  Aquaporin 5, membrane.
Mentions: AQP-5 steady state levels in cytoplasm and membranes was significantly greater in both groups (2H and 4H) vs. the controls. Besides, AQP-5 expression on cytoplasm and membranes was greater in group 4H than in group 2H (p = 0.027 and p = 0.039, respectively) (Figs. 5 and 7). To better characterize these differences in the expression of AQP 5, a regression analysis of both variables was conducted, which provided a coefficient of determination R2 = 0.80 (p = 0.008) and R2 = 0.90 (p = 0.001) (Fig. 8).

Bottom Line: Lung oedema and alveolar-capillary membrane permeability did not change during MV.But in AQP-1 expression these differences were not found.AQPs may have a protective effect against the oedema induced by MV.

View Article: PubMed Central - PubMed

Affiliation: Hospital General Universitario, Valencia, Spain.

ABSTRACT

Background and goals: Mechanical ventilation (MV) can induce or worsen pulmonary oedema. Aquaporins (AQPs) facilitate the selective and rapid bi-directional movement of water. Their role in the development and resolution of pulmonary oedema is controversial. Our objectives are to determine if prolonged MV causes lung oedema and changes in the expression of AQP 1 and AQP 5 in rats.

Methods: 25 male Wistar rats were subjected to MV with a tidal volume of 10 ml/kg, during 2 hours (n = 12) and 4 hours (n = 13). Degree of oedema was compared with a group of non-ventilated rats (n = 5). The expression of AQP 1 and AQP 5 were determined by western immunoblotting, measuring the amount of mRNA (previously amplified by RT-PCR) and immunohistochemical staining of AQPs 1 and 5 in lung samples from all groups.

Results: Lung oedema and alveolar-capillary membrane permeability did not change during MV. AQP-5 steady state levels in the western blot were increased (p<0.01) at 2 h and 4 h of MV. But in AQP-1 expression these differences were not found. However, the amount of mRNA for AQP-1 was increased at 2 h and 4 h of MV; and for AQP 5 at 4 h of MV. These findings were corroborated by representative immunohistochemical lung samples.

Conclusion: In lungs from rats ventilated with a low tidal volume the expression of AQP 5 increases gradually with MV duration, but does not cause pulmonary oedema or changes in lung permeability. AQPs may have a protective effect against the oedema induced by MV.

Show MeSH
Related in: MedlinePlus