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The four-herb Chinese medicine ANBP enhances wound healing and inhibits scar formation via bidirectional regulation of transformation growth factor pathway.

Hou Q, He WJ, Hao HJ, Han QW, Chen L, Dong L, Liu JJ, Li X, Zhang YJ, Ma YZ, Han WD, Fu XB - PLoS ONE (2014)

Bottom Line: We demonstrated that these effects of ANBP are associated with transforming growth factor (TGF)-β1-mediated signalling pathways through Smad-dependent pathways.ANBP treatment significantly increased expression of TGF-β1 and Smad2/3 mRNA at the early stage of wound healing, and led to markedly decrease expression of TGF-β1 and Smad2/3 compared with the control group after 14 days post-wounding.Taken together, our results defined a bidirectional regulation role of ANBP for TGF-β1/Smad pathway in promoting wound healing and alleviating scar formation, which may be an effective therapy for human wounds at the earliest stage.

View Article: PubMed Central - PubMed

Affiliation: Institute of Basic Medicine, Chinese PLA General Hospital, Beijing, P. R. China; Department of Internal Medicine, Chinese PLA 148th Hospital, Zibo, P. R. China.

ABSTRACT
The four-herb Chinese medicine ANBP is a pulverized mixture of four herbs including Agrimonia Eupatoria (A), Nelumbo Nucifera Gaertn (N), Boswellia Carteri (B) and Pollen Typhae Angustifoliae (P). The combination of the four herbs was first described in Chinese canonical medicine about 2000 years ago for treatment of various trauma disorders, such as hemostasis, antiinflammatory, analgesia, and wound healing, etc. However, the precise mechanisms of ANBP are still unclear. In our study, using rabbit ear hypertrophic scar models of full-thickness skin defect, we showed that local ANBP treatment not only significantly enhanced wound healing by relieving inflammation, increasing formation of granulation tissue and accelerating re-epithelialization, but also reduced scar formation by decreasing collagen production, protuberant height and volume of scars, and increasing collagen maturity. We demonstrated that these effects of ANBP are associated with transforming growth factor (TGF)-β1-mediated signalling pathways through Smad-dependent pathways. ANBP treatment significantly increased expression of TGF-β1 and Smad2/3 mRNA at the early stage of wound healing, and led to markedly decrease expression of TGF-β1 and Smad2/3 compared with the control group after 14 days post-wounding. Taken together, our results defined a bidirectional regulation role of ANBP for TGF-β1/Smad pathway in promoting wound healing and alleviating scar formation, which may be an effective therapy for human wounds at the earliest stage.

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Changes in expression of inflammatory factors and collagen after ANBP treatment.mRNA levels were detected by quantitative RT-PCR at 4, 7, 14 and 28 days post-wounding. mRNA levels are shown for: (A) IL-1; (B) TNF-α; (C) Col I; (D) Col III. Expression levels were calculated using standard curves and normalized to expression level of β-actin as an endogenous reference gene. * p<0.05, ** p<0.01.
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pone-0112274-g005: Changes in expression of inflammatory factors and collagen after ANBP treatment.mRNA levels were detected by quantitative RT-PCR at 4, 7, 14 and 28 days post-wounding. mRNA levels are shown for: (A) IL-1; (B) TNF-α; (C) Col I; (D) Col III. Expression levels were calculated using standard curves and normalized to expression level of β-actin as an endogenous reference gene. * p<0.05, ** p<0.01.

Mentions: Inflammation is an important factor affecting the outcome of wound healing. Wounding induced large increases in IL-1 and TNF-α mRNA expression. Real-time PCR indicated that both were highest at the earliest time point examined (4 days post-wounding), declining thereafter over the time of healing (Fig. 5A and 5B). ANBP treatment significantly reduced the levels of inflammatory cytokines IL-1 and TNF-α at all time points. The mRNA levels of collagen I and collagen III increased further over time (Fig. 5C and 5D). Compared with the control, ANBP treatment slightly increased collagen I and collagen III synthesis during the early post-wounding period (7 days). But at the later time of healing (14 days), expressions of collagen I and collagen III were both reduced by ANBP treatment.


The four-herb Chinese medicine ANBP enhances wound healing and inhibits scar formation via bidirectional regulation of transformation growth factor pathway.

Hou Q, He WJ, Hao HJ, Han QW, Chen L, Dong L, Liu JJ, Li X, Zhang YJ, Ma YZ, Han WD, Fu XB - PLoS ONE (2014)

Changes in expression of inflammatory factors and collagen after ANBP treatment.mRNA levels were detected by quantitative RT-PCR at 4, 7, 14 and 28 days post-wounding. mRNA levels are shown for: (A) IL-1; (B) TNF-α; (C) Col I; (D) Col III. Expression levels were calculated using standard curves and normalized to expression level of β-actin as an endogenous reference gene. * p<0.05, ** p<0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4260828&req=5

pone-0112274-g005: Changes in expression of inflammatory factors and collagen after ANBP treatment.mRNA levels were detected by quantitative RT-PCR at 4, 7, 14 and 28 days post-wounding. mRNA levels are shown for: (A) IL-1; (B) TNF-α; (C) Col I; (D) Col III. Expression levels were calculated using standard curves and normalized to expression level of β-actin as an endogenous reference gene. * p<0.05, ** p<0.01.
Mentions: Inflammation is an important factor affecting the outcome of wound healing. Wounding induced large increases in IL-1 and TNF-α mRNA expression. Real-time PCR indicated that both were highest at the earliest time point examined (4 days post-wounding), declining thereafter over the time of healing (Fig. 5A and 5B). ANBP treatment significantly reduced the levels of inflammatory cytokines IL-1 and TNF-α at all time points. The mRNA levels of collagen I and collagen III increased further over time (Fig. 5C and 5D). Compared with the control, ANBP treatment slightly increased collagen I and collagen III synthesis during the early post-wounding period (7 days). But at the later time of healing (14 days), expressions of collagen I and collagen III were both reduced by ANBP treatment.

Bottom Line: We demonstrated that these effects of ANBP are associated with transforming growth factor (TGF)-β1-mediated signalling pathways through Smad-dependent pathways.ANBP treatment significantly increased expression of TGF-β1 and Smad2/3 mRNA at the early stage of wound healing, and led to markedly decrease expression of TGF-β1 and Smad2/3 compared with the control group after 14 days post-wounding.Taken together, our results defined a bidirectional regulation role of ANBP for TGF-β1/Smad pathway in promoting wound healing and alleviating scar formation, which may be an effective therapy for human wounds at the earliest stage.

View Article: PubMed Central - PubMed

Affiliation: Institute of Basic Medicine, Chinese PLA General Hospital, Beijing, P. R. China; Department of Internal Medicine, Chinese PLA 148th Hospital, Zibo, P. R. China.

ABSTRACT
The four-herb Chinese medicine ANBP is a pulverized mixture of four herbs including Agrimonia Eupatoria (A), Nelumbo Nucifera Gaertn (N), Boswellia Carteri (B) and Pollen Typhae Angustifoliae (P). The combination of the four herbs was first described in Chinese canonical medicine about 2000 years ago for treatment of various trauma disorders, such as hemostasis, antiinflammatory, analgesia, and wound healing, etc. However, the precise mechanisms of ANBP are still unclear. In our study, using rabbit ear hypertrophic scar models of full-thickness skin defect, we showed that local ANBP treatment not only significantly enhanced wound healing by relieving inflammation, increasing formation of granulation tissue and accelerating re-epithelialization, but also reduced scar formation by decreasing collagen production, protuberant height and volume of scars, and increasing collagen maturity. We demonstrated that these effects of ANBP are associated with transforming growth factor (TGF)-β1-mediated signalling pathways through Smad-dependent pathways. ANBP treatment significantly increased expression of TGF-β1 and Smad2/3 mRNA at the early stage of wound healing, and led to markedly decrease expression of TGF-β1 and Smad2/3 compared with the control group after 14 days post-wounding. Taken together, our results defined a bidirectional regulation role of ANBP for TGF-β1/Smad pathway in promoting wound healing and alleviating scar formation, which may be an effective therapy for human wounds at the earliest stage.

Show MeSH
Related in: MedlinePlus