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In vitro antioxidant activity and in vivo antifatigue effect of layered double hydroxide nanoparticles as delivery vehicles for folic acid.

Qin L, Wang W, You S, Dong J, Zhou Y, Wang J - Int J Nanomedicine (2014)

Bottom Line: The resultant folic acid-LDH constructs were then characterized by X-ray powder diffraction and transmission electron microscopy.The results showed that folic acid-LDH antioxidant system can scavenge 1,1-diphenyl-2-picrylhydrazyl and hydroxyl free radicals and chelate pro-oxidative Cu(2+).It had an obvious effect on decreasing the blood urea nitrogen and blood lactic acid, while increasing muscle and hepatic glycogen levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Education, Tongji University, Shanghai, People's Republic of China.

ABSTRACT
Folic acid antioxidants were successfully intercalated into layered double hydroxides (LDH) nanoparticles according to a previous method with minor modification. The resultant folic acid-LDH constructs were then characterized by X-ray powder diffraction and transmission electron microscopy. The in vitro antioxidant activities, cytotoxicity effect, and in vivo antifatigue were examined by a series of assays. The results showed that folic acid-LDH antioxidant system can scavenge 1,1-diphenyl-2-picrylhydrazyl and hydroxyl free radicals and chelate pro-oxidative Cu(2+). The in vitro cytotoxicity assays indicated that folic acid-LDH antioxidant system had no significant cytotoxic effect or obvious toxicity to normal cells. It also prolonged the forced swimming time of the mice by 32% and 51% compared to folic acid and control groups, respectively. It had an obvious effect on decreasing the blood urea nitrogen and blood lactic acid, while increasing muscle and hepatic glycogen levels. Therefore, folic acid-LDH might be used as a novel antioxidant and antifatigue nutritional supplement.

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Related in: MedlinePlus

Cell viability of LDH, folic acid, and folic acid-LDH on the growth of ges-1 cells.Notes: The cells have been treated with one of these three agents for 48 hours at concentrations of 5, 10, 20, and 40 μg/mL. Results represent the means of three independent experiments and error bars represent the standard error of the mean. N=3, *P<0.05; **P<0.001. All entries showed significant difference with the control group according to the student’s t-test.Abbreviation: LDH, layered double hydroxide.
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f7-ijn-9-5701: Cell viability of LDH, folic acid, and folic acid-LDH on the growth of ges-1 cells.Notes: The cells have been treated with one of these three agents for 48 hours at concentrations of 5, 10, 20, and 40 μg/mL. Results represent the means of three independent experiments and error bars represent the standard error of the mean. N=3, *P<0.05; **P<0.001. All entries showed significant difference with the control group according to the student’s t-test.Abbreviation: LDH, layered double hydroxide.

Mentions: The cytotoxicity of pure folic acid and folic acid-LDH were evaluated in GES-1 using MTT tetrazolium assay. The yellow tetrazolium MTT was reduced by metabolically active cells, in part by the action of hydrogenase enzymes to generate reducing equivalents such as NADH and NADPH. The resulting intracellular purple formazan was solubilized and quantized by spectrophotometric means. Figure 7 shows the effect of folic acid and folic acid-LDH on the proliferation of GES-1 cells at various concentrations. Both pure drug folic acid and folic acid-LDH nanoparticles do not show an obvious toxicity to GES-1 cells owing to the fact that the rate of living cells is maintained at 90% after incubation for 48 hours at each of the tested concentrations. Therefore, this confirms that folic acid-LDH is hardly toxic to GES-1 cells and can serve as a safe drug-delivery system in the cellular system.


In vitro antioxidant activity and in vivo antifatigue effect of layered double hydroxide nanoparticles as delivery vehicles for folic acid.

Qin L, Wang W, You S, Dong J, Zhou Y, Wang J - Int J Nanomedicine (2014)

Cell viability of LDH, folic acid, and folic acid-LDH on the growth of ges-1 cells.Notes: The cells have been treated with one of these three agents for 48 hours at concentrations of 5, 10, 20, and 40 μg/mL. Results represent the means of three independent experiments and error bars represent the standard error of the mean. N=3, *P<0.05; **P<0.001. All entries showed significant difference with the control group according to the student’s t-test.Abbreviation: LDH, layered double hydroxide.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4260686&req=5

f7-ijn-9-5701: Cell viability of LDH, folic acid, and folic acid-LDH on the growth of ges-1 cells.Notes: The cells have been treated with one of these three agents for 48 hours at concentrations of 5, 10, 20, and 40 μg/mL. Results represent the means of three independent experiments and error bars represent the standard error of the mean. N=3, *P<0.05; **P<0.001. All entries showed significant difference with the control group according to the student’s t-test.Abbreviation: LDH, layered double hydroxide.
Mentions: The cytotoxicity of pure folic acid and folic acid-LDH were evaluated in GES-1 using MTT tetrazolium assay. The yellow tetrazolium MTT was reduced by metabolically active cells, in part by the action of hydrogenase enzymes to generate reducing equivalents such as NADH and NADPH. The resulting intracellular purple formazan was solubilized and quantized by spectrophotometric means. Figure 7 shows the effect of folic acid and folic acid-LDH on the proliferation of GES-1 cells at various concentrations. Both pure drug folic acid and folic acid-LDH nanoparticles do not show an obvious toxicity to GES-1 cells owing to the fact that the rate of living cells is maintained at 90% after incubation for 48 hours at each of the tested concentrations. Therefore, this confirms that folic acid-LDH is hardly toxic to GES-1 cells and can serve as a safe drug-delivery system in the cellular system.

Bottom Line: The resultant folic acid-LDH constructs were then characterized by X-ray powder diffraction and transmission electron microscopy.The results showed that folic acid-LDH antioxidant system can scavenge 1,1-diphenyl-2-picrylhydrazyl and hydroxyl free radicals and chelate pro-oxidative Cu(2+).It had an obvious effect on decreasing the blood urea nitrogen and blood lactic acid, while increasing muscle and hepatic glycogen levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Education, Tongji University, Shanghai, People's Republic of China.

ABSTRACT
Folic acid antioxidants were successfully intercalated into layered double hydroxides (LDH) nanoparticles according to a previous method with minor modification. The resultant folic acid-LDH constructs were then characterized by X-ray powder diffraction and transmission electron microscopy. The in vitro antioxidant activities, cytotoxicity effect, and in vivo antifatigue were examined by a series of assays. The results showed that folic acid-LDH antioxidant system can scavenge 1,1-diphenyl-2-picrylhydrazyl and hydroxyl free radicals and chelate pro-oxidative Cu(2+). The in vitro cytotoxicity assays indicated that folic acid-LDH antioxidant system had no significant cytotoxic effect or obvious toxicity to normal cells. It also prolonged the forced swimming time of the mice by 32% and 51% compared to folic acid and control groups, respectively. It had an obvious effect on decreasing the blood urea nitrogen and blood lactic acid, while increasing muscle and hepatic glycogen levels. Therefore, folic acid-LDH might be used as a novel antioxidant and antifatigue nutritional supplement.

Show MeSH
Related in: MedlinePlus