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Effects of a simulated CO2 pneumoperitoneum environment on the proliferation, apoptosis, and metastasis of cervical cancer cells in vitro.

Lin F, Pan L, Li L, Li D, Mo L - Med. Sci. Monit. (2014)

Bottom Line: The cells in the control group were cultured in a standard environment.The growth curve was drawn through constant survival cell counts for 7 days, and the group with most obvious change was selected for subsequent experiments to detect cell colony formation, cell cycle apoptosis, and anti-anoikis, and the ability of invasion, adhesion, and migration.Compared with the control group, the early apoptosis rate in the experimental group was higher, and the ability of invasion, migration, and adhesion decreased significantly.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Tumor Hospital of Guangxi Medical University, Nanning, China (mainland).

ABSTRACT

Background: This study aimed to investigate the growth curve, cell colony formation, cell cycle, apoptosis, anti-anoikis, and ability of invasion, adhesion, and migration of cervical cancer cells after exposure to a model of a simulated CO2 pneumoperitoneum environment with different pressures and at different times.

Material and methods: The cervical cancer cells were cultured in groups with 8 and 16 mmHg of 100% CO2 for 1, 2, 3, and 4 h in a model of a simulated environment of CO2 pneumoperitoneum. The cells in the control group were cultured in a standard environment. The growth curve was drawn through constant survival cell counts for 7 days, and the group with most obvious change was selected for subsequent experiments to detect cell colony formation, cell cycle apoptosis, and anti-anoikis, and the ability of invasion, adhesion, and migration.

Results: After a brief inhibition, the proliferation of cervical cancer cells was markedly increased and had no relationship with different CO2 pressures. Compared with the control group, the early apoptosis rate in the experimental group was higher, and the ability of invasion, migration, and adhesion decreased significantly.

Conclusions: Cervical cancer cells stimulated by a CO2 pneumoperitoneum environment in vitro have an increased the ability to proliferate after a short period of inhibition and have reduced abilities of invasion, migration, and adhesion.

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Related in: MedlinePlus

The Hele cells passed through the membrane surface of the transwell chambers in the cell migration experiment in the 1th day. (A) control group, (B) (16 mmHg, 4 h) group.
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f5-medscimonit-20-2497: The Hele cells passed through the membrane surface of the transwell chambers in the cell migration experiment in the 1th day. (A) control group, (B) (16 mmHg, 4 h) group.

Mentions: Figures 4 and 5 show that the HeLa cells passed through the membrane surface of the transwell chambers in the cell invasion and migration experiment. Compared with the control group, the ability of cell invasion in the experimental groups was inhibited after treatment with CO2 (P<0.05) (Table 3), the cell migration ability in the experimental group was inhibited at 1, 3, and 5 days after stimulation by CO2 (P<0.05), and there was no significant difference on the 7th day (Table 4). There were significant differences in A450 nm value of MTT between the experimental group and the control group at 1 and 3 days (P<0.05), and there was no significant difference on the 5th and 7th days (Table 5).


Effects of a simulated CO2 pneumoperitoneum environment on the proliferation, apoptosis, and metastasis of cervical cancer cells in vitro.

Lin F, Pan L, Li L, Li D, Mo L - Med. Sci. Monit. (2014)

The Hele cells passed through the membrane surface of the transwell chambers in the cell migration experiment in the 1th day. (A) control group, (B) (16 mmHg, 4 h) group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4260668&req=5

f5-medscimonit-20-2497: The Hele cells passed through the membrane surface of the transwell chambers in the cell migration experiment in the 1th day. (A) control group, (B) (16 mmHg, 4 h) group.
Mentions: Figures 4 and 5 show that the HeLa cells passed through the membrane surface of the transwell chambers in the cell invasion and migration experiment. Compared with the control group, the ability of cell invasion in the experimental groups was inhibited after treatment with CO2 (P<0.05) (Table 3), the cell migration ability in the experimental group was inhibited at 1, 3, and 5 days after stimulation by CO2 (P<0.05), and there was no significant difference on the 7th day (Table 4). There were significant differences in A450 nm value of MTT between the experimental group and the control group at 1 and 3 days (P<0.05), and there was no significant difference on the 5th and 7th days (Table 5).

Bottom Line: The cells in the control group were cultured in a standard environment.The growth curve was drawn through constant survival cell counts for 7 days, and the group with most obvious change was selected for subsequent experiments to detect cell colony formation, cell cycle apoptosis, and anti-anoikis, and the ability of invasion, adhesion, and migration.Compared with the control group, the early apoptosis rate in the experimental group was higher, and the ability of invasion, migration, and adhesion decreased significantly.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Tumor Hospital of Guangxi Medical University, Nanning, China (mainland).

ABSTRACT

Background: This study aimed to investigate the growth curve, cell colony formation, cell cycle, apoptosis, anti-anoikis, and ability of invasion, adhesion, and migration of cervical cancer cells after exposure to a model of a simulated CO2 pneumoperitoneum environment with different pressures and at different times.

Material and methods: The cervical cancer cells were cultured in groups with 8 and 16 mmHg of 100% CO2 for 1, 2, 3, and 4 h in a model of a simulated environment of CO2 pneumoperitoneum. The cells in the control group were cultured in a standard environment. The growth curve was drawn through constant survival cell counts for 7 days, and the group with most obvious change was selected for subsequent experiments to detect cell colony formation, cell cycle apoptosis, and anti-anoikis, and the ability of invasion, adhesion, and migration.

Results: After a brief inhibition, the proliferation of cervical cancer cells was markedly increased and had no relationship with different CO2 pressures. Compared with the control group, the early apoptosis rate in the experimental group was higher, and the ability of invasion, migration, and adhesion decreased significantly.

Conclusions: Cervical cancer cells stimulated by a CO2 pneumoperitoneum environment in vitro have an increased the ability to proliferate after a short period of inhibition and have reduced abilities of invasion, migration, and adhesion.

Show MeSH
Related in: MedlinePlus