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Role of the sympathetic nervous system and spleen in experimental stroke-induced immunodepression.

Yan FL, Zhang JH - Med. Sci. Monit. (2014)

Bottom Line: In addition, rat spleen size was reduced.Correlation analysis indicated that MCAO-induced spleen size reduction correlated with the changes in MN, NMN, and cytokines.Blocking SNS with propranolol can partly reverse the immunodepression and the reduction in spleen volume.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Zhong-Da Hospital, Southeast University, Nanjing, China (mainland).

ABSTRACT

Background: The mechanism of stroke-induced immunodepression syndrome (SIDS) remains uncertain. Some studies suggest that hyperactivation of the sympathetic nervous system (SNS) may be the key factor underlying SIDS. Catecholamines impair early lymphocyte response, which can increase the risk of stroke-associated infection (SAI).

Material/methods: Our study focused on dynamic changes of metanephrine (MN), normetanephrine (NMN), cytokines, and spleen volume in the rat middle cerebral artery occlusion (MCAO) model.

Results: After MCAO, there is hyperactivation of SNS and pro-/anti-inflammatory imbalance, indicating systemic immunodepression. In addition, rat spleen size was reduced. Correlation analysis indicated that MCAO-induced spleen size reduction correlated with the changes in MN, NMN, and cytokines. Blocking SNS with propranolol can partly reverse the immunodepression and the reduction in spleen volume.

Conclusions: Taken together, these findings suggest that acute ischemic stroke induces over-activation of the SNS, which lowers the threshold of infection and increases the risk of infection.

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Related in: MedlinePlus

TTC stain: cerebral infarction area is white, and normal brain tissue is stained red.
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f2-medscimonit-20-2489: TTC stain: cerebral infarction area is white, and normal brain tissue is stained red.

Mentions: After the collection of blood samples, all rats were killed by decapitation and the brains were quickly removed. Six 2.0-mm-thick coronal slices of brain were prepared with a brain matrix (ASI Instruments, Inc) and then were stained with 0.1% 2,3,5-triphenyltetrazolium chloride (TTC, Sigma-Aldrich, USA) in saline at 37°C for 15 min, as described previously [10]. On each slice, the unstained area represents ischemic brain (Figure 2). Subsequently, hematoxylin and eosin (HE) staining was also performed on these slices (Figure 3).


Role of the sympathetic nervous system and spleen in experimental stroke-induced immunodepression.

Yan FL, Zhang JH - Med. Sci. Monit. (2014)

TTC stain: cerebral infarction area is white, and normal brain tissue is stained red.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4260620&req=5

f2-medscimonit-20-2489: TTC stain: cerebral infarction area is white, and normal brain tissue is stained red.
Mentions: After the collection of blood samples, all rats were killed by decapitation and the brains were quickly removed. Six 2.0-mm-thick coronal slices of brain were prepared with a brain matrix (ASI Instruments, Inc) and then were stained with 0.1% 2,3,5-triphenyltetrazolium chloride (TTC, Sigma-Aldrich, USA) in saline at 37°C for 15 min, as described previously [10]. On each slice, the unstained area represents ischemic brain (Figure 2). Subsequently, hematoxylin and eosin (HE) staining was also performed on these slices (Figure 3).

Bottom Line: In addition, rat spleen size was reduced.Correlation analysis indicated that MCAO-induced spleen size reduction correlated with the changes in MN, NMN, and cytokines.Blocking SNS with propranolol can partly reverse the immunodepression and the reduction in spleen volume.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Zhong-Da Hospital, Southeast University, Nanjing, China (mainland).

ABSTRACT

Background: The mechanism of stroke-induced immunodepression syndrome (SIDS) remains uncertain. Some studies suggest that hyperactivation of the sympathetic nervous system (SNS) may be the key factor underlying SIDS. Catecholamines impair early lymphocyte response, which can increase the risk of stroke-associated infection (SAI).

Material/methods: Our study focused on dynamic changes of metanephrine (MN), normetanephrine (NMN), cytokines, and spleen volume in the rat middle cerebral artery occlusion (MCAO) model.

Results: After MCAO, there is hyperactivation of SNS and pro-/anti-inflammatory imbalance, indicating systemic immunodepression. In addition, rat spleen size was reduced. Correlation analysis indicated that MCAO-induced spleen size reduction correlated with the changes in MN, NMN, and cytokines. Blocking SNS with propranolol can partly reverse the immunodepression and the reduction in spleen volume.

Conclusions: Taken together, these findings suggest that acute ischemic stroke induces over-activation of the SNS, which lowers the threshold of infection and increases the risk of infection.

Show MeSH
Related in: MedlinePlus