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Impact of sperm chromatin evaluation on fertilization rate in intracytoplasmic sperm injection.

Iranpour FG - Adv Biomed Res (2014)

Bottom Line: Sperm DNA in human beings and most vertebrates is packed by protamines into highly compact form of chromatin.Percentage of CMA3 positivity, unlike those of aniline blue and acridine orange, showed significant negative correlation with fertilization rate.By dividing patients into CMA3 <48% and CMA3> 48% groups, the area under the curve was 0.646.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: Sperm DNA in human beings and most vertebrates is packed by protamines into highly compact form of chromatin. There are many staining methods to assess sperm chromatin. Three different methods of staining were used simultaneously in this study and the goal was to determine which of these sperm tests has a relation with fertilization rate in intracytoplasmic sperm injection (ICSI).

Materials and methods: Thirty couples who referred to Yamagata University Hospital (Yamagata, Japan) for ICSI were included in this study. The greater part of semen was prepared for ICSI. The remaining part was used for staining with aniline blue, acridine orange, and chromomycin A3 (CMA3). For evaluation of abnormal morphology and abnormality of head, Papanicolaou-stained smears were used. The analysis of data was done using Spearman coefficient of correlation and logistic regression model. Receiver operator characteristic (ROC) curve was used for discrimination of CMA3 staining power to identify ICSI rates.

Results: Percentage of CMA3 positivity, unlike those of aniline blue and acridine orange, showed significant negative correlation with fertilization rate. Moreover, the percentage of CMA3 positivity showed a positive correlation with the percentage of abnormal morphology and abnormality of head. By dividing patients into CMA3 <48% and CMA3> 48% groups, the area under the curve was 0.646.

Conclusions: CMA3 staining (protamine deficiency) could be considered as a useful tool for evaluation of male fertility prior to infertility treatment.

No MeSH data available.


Related in: MedlinePlus

Receiver operator characteristic curve for fertilization rate based on CMA3 staining
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Figure 1: Receiver operator characteristic curve for fertilization rate based on CMA3 staining

Mentions: As CMA3 positivity can affect the fertilization rate independently, the thresholds that have been suggested by Esterhuizen et al.[14] were chosen. Patients were grouped into CMA3 levels of < 48% and > 48%. The ROC analysis showed that the area under the curve was 0.646, which indicates that CMA3 is a moderate diagnostic factor [Figure 1].


Impact of sperm chromatin evaluation on fertilization rate in intracytoplasmic sperm injection.

Iranpour FG - Adv Biomed Res (2014)

Receiver operator characteristic curve for fertilization rate based on CMA3 staining
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4260290&req=5

Figure 1: Receiver operator characteristic curve for fertilization rate based on CMA3 staining
Mentions: As CMA3 positivity can affect the fertilization rate independently, the thresholds that have been suggested by Esterhuizen et al.[14] were chosen. Patients were grouped into CMA3 levels of < 48% and > 48%. The ROC analysis showed that the area under the curve was 0.646, which indicates that CMA3 is a moderate diagnostic factor [Figure 1].

Bottom Line: Sperm DNA in human beings and most vertebrates is packed by protamines into highly compact form of chromatin.Percentage of CMA3 positivity, unlike those of aniline blue and acridine orange, showed significant negative correlation with fertilization rate.By dividing patients into CMA3 <48% and CMA3> 48% groups, the area under the curve was 0.646.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: Sperm DNA in human beings and most vertebrates is packed by protamines into highly compact form of chromatin. There are many staining methods to assess sperm chromatin. Three different methods of staining were used simultaneously in this study and the goal was to determine which of these sperm tests has a relation with fertilization rate in intracytoplasmic sperm injection (ICSI).

Materials and methods: Thirty couples who referred to Yamagata University Hospital (Yamagata, Japan) for ICSI were included in this study. The greater part of semen was prepared for ICSI. The remaining part was used for staining with aniline blue, acridine orange, and chromomycin A3 (CMA3). For evaluation of abnormal morphology and abnormality of head, Papanicolaou-stained smears were used. The analysis of data was done using Spearman coefficient of correlation and logistic regression model. Receiver operator characteristic (ROC) curve was used for discrimination of CMA3 staining power to identify ICSI rates.

Results: Percentage of CMA3 positivity, unlike those of aniline blue and acridine orange, showed significant negative correlation with fertilization rate. Moreover, the percentage of CMA3 positivity showed a positive correlation with the percentage of abnormal morphology and abnormality of head. By dividing patients into CMA3 <48% and CMA3> 48% groups, the area under the curve was 0.646.

Conclusions: CMA3 staining (protamine deficiency) could be considered as a useful tool for evaluation of male fertility prior to infertility treatment.

No MeSH data available.


Related in: MedlinePlus