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Effects of biosurfactant produced by Lactobacillus casei on gtfB, gtfC, and ftf gene expression level in S. mutans by real-time RT-PCR.

Savabi O, Kazemi M, Kamali S, Salehi AR, Eslami G, Tahmourespour A, Salehi R - Adv Biomed Res (2014)

Bottom Line: The application of the prepared biosurfactant caused dramatic down regulation of all the three genes under study.The reduction in gene expression was statistically highly significant (for gtfB, P > 0.0002; for gtfC, P > 0.0063, and for ftf, P > 0.0057).In view of the importance of glucosyltransferase gene products for S.mutans attachment to the tooth surface which is the initial important step in biofilm production and dental caries, further research in this field may lead to an applicable alternative for successful with least adverse side effects in dental caries prevention.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Prosthetics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: The Streptococci are the pioneer strains in plaque formation and Streptococcus mutans are the main etiological agent of dental plaque and caries. In general, biofilm formation is a step-wise process, which begins by adhesion of planktonic cells to the surfaces. Evidences show that expression of glucosyltransferase B and C (gtfB and gtfC) and fructosyltransferase (ftf) genes play critical role in initial adhesion of S. mutans to the tooth surface which results in formation of dental plaques and consequently caries and other periodontal disease.

Materials and methods: The aim of this study was to determine the effect of biosurfactants produced by a probiotic strain; Lactobacillus casei (ATCC39392) on gene expression profile of gftB/C and tft of S. mutans (ATCC35668) using quantitative real-time PCR.

Results: The application of the prepared biosurfactant caused dramatic down regulation of all the three genes under study. The reduction in gene expression was statistically highly significant (for gtfB, P > 0.0002; for gtfC, P > 0.0063, and for ftf, P > 0.0057).

Conclusion: Considerable downregulation of all three genes in the presence of the prepared biosurfactant comparing to untreated controls is indicative of successful inhibition of influential genes in bacterial adhesion phenomena. In view of the importance of glucosyltransferase gene products for S.mutans attachment to the tooth surface which is the initial important step in biofilm production and dental caries, further research in this field may lead to an applicable alternative for successful with least adverse side effects in dental caries prevention.

No MeSH data available.


Related in: MedlinePlus

The effect of L.c.-derived biosurfactant on gtfB/C and ftf gene expression level in S. mutans ATCC35668
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Figure 2: The effect of L.c.-derived biosurfactant on gtfB/C and ftf gene expression level in S. mutans ATCC35668

Mentions: The 16S rRNA gene was used as an endogenous reference [Figure 2]. The biosurfactant significantly reduced expression of all three genes under study in comparison to the reference gene. The P values obtained for each of the three genes (for gtfB, P > o.ooo2; for gtfC, P > 0.0063; and for ftf, P > 0.0057), is indicative of the high efficiency of the produced biosurfactant in downregulation of these adhesive promoting genes of S. mutans.


Effects of biosurfactant produced by Lactobacillus casei on gtfB, gtfC, and ftf gene expression level in S. mutans by real-time RT-PCR.

Savabi O, Kazemi M, Kamali S, Salehi AR, Eslami G, Tahmourespour A, Salehi R - Adv Biomed Res (2014)

The effect of L.c.-derived biosurfactant on gtfB/C and ftf gene expression level in S. mutans ATCC35668
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4260286&req=5

Figure 2: The effect of L.c.-derived biosurfactant on gtfB/C and ftf gene expression level in S. mutans ATCC35668
Mentions: The 16S rRNA gene was used as an endogenous reference [Figure 2]. The biosurfactant significantly reduced expression of all three genes under study in comparison to the reference gene. The P values obtained for each of the three genes (for gtfB, P > o.ooo2; for gtfC, P > 0.0063; and for ftf, P > 0.0057), is indicative of the high efficiency of the produced biosurfactant in downregulation of these adhesive promoting genes of S. mutans.

Bottom Line: The application of the prepared biosurfactant caused dramatic down regulation of all the three genes under study.The reduction in gene expression was statistically highly significant (for gtfB, P > 0.0002; for gtfC, P > 0.0063, and for ftf, P > 0.0057).In view of the importance of glucosyltransferase gene products for S.mutans attachment to the tooth surface which is the initial important step in biofilm production and dental caries, further research in this field may lead to an applicable alternative for successful with least adverse side effects in dental caries prevention.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Prosthetics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: The Streptococci are the pioneer strains in plaque formation and Streptococcus mutans are the main etiological agent of dental plaque and caries. In general, biofilm formation is a step-wise process, which begins by adhesion of planktonic cells to the surfaces. Evidences show that expression of glucosyltransferase B and C (gtfB and gtfC) and fructosyltransferase (ftf) genes play critical role in initial adhesion of S. mutans to the tooth surface which results in formation of dental plaques and consequently caries and other periodontal disease.

Materials and methods: The aim of this study was to determine the effect of biosurfactants produced by a probiotic strain; Lactobacillus casei (ATCC39392) on gene expression profile of gftB/C and tft of S. mutans (ATCC35668) using quantitative real-time PCR.

Results: The application of the prepared biosurfactant caused dramatic down regulation of all the three genes under study. The reduction in gene expression was statistically highly significant (for gtfB, P > 0.0002; for gtfC, P > 0.0063, and for ftf, P > 0.0057).

Conclusion: Considerable downregulation of all three genes in the presence of the prepared biosurfactant comparing to untreated controls is indicative of successful inhibition of influential genes in bacterial adhesion phenomena. In view of the importance of glucosyltransferase gene products for S.mutans attachment to the tooth surface which is the initial important step in biofilm production and dental caries, further research in this field may lead to an applicable alternative for successful with least adverse side effects in dental caries prevention.

No MeSH data available.


Related in: MedlinePlus