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Ductal carcinoma in situ of the breast: correlation between histopathological features and age of patients.

Perez AA, Balabram D, Salles Mde A, Gobbi H - Diagn Pathol (2014)

Bottom Line: Only 32% of tumours with a cribriform pattern had high nuclear grade.Comedonecrosis was more common in the solid morphology than in the cribriform, papillary and micropapillary subtypes (p < 0.001).Fair agreement was observed between in situ and invasive components with regard to grade (weighted kappa = 0.23).

View Article: PubMed Central - PubMed

Affiliation: Breast Pathology Laboratory, School of Medicine, Federal University of Minas Gerais, Av. Prof. Alfredo Balena, 190, Belo Horizonte, Minas Gerais, Brasil. amandaperez1408@yahoo.com.br.

ABSTRACT

Background: The histopathological subtype, nuclear grade and presence or absence of comedonecrosis are established as critical elements in the reporting of ductal carcinoma in situ (DCIS) of the breast. The aims of this study were to determine the frequencies of morphological subtypes of DCIS, nuclear grade and comedonecrosis; to compare the age of patients with the histopathological characteristics of DCIS, and to assess the agreement of grade between in situ and invasive components in DCIS cases that were associated with invasive carcinoma.

Methods: We evaluated a series of 403 cases of DCIS, pure or associated with invasive mammary carcinoma, consecutively identified from the histopathology files of the Breast Pathology Laboratory, Federal University of Minas Gerais, Brazil, from 2003 to 2008.

Results: DCIS displayed a single growth pattern in most cases (55.1%) and the solid subtype was the most common morphology (42.2% of the total). High-grade DCIS was identified in 293/403 cases (72.7%) and comedonecrosis was present in 222/403 cases (55%). Among DCIS with a single architectural pattern, high grade was more common in the solid subtype (151/168 cases, 89.9%; p < 0.001). Only 32% of tumours with a cribriform pattern had high nuclear grade. Comedonecrosis was more common in the solid morphology than in the cribriform, papillary and micropapillary subtypes (p < 0.001). Patients with high-grade DCIS were younger in relation to patients with low-grade DCIS (p = 0.027) and patients with tumours with comedonecrosis were also younger in comparison to patients with tumours without comedonecrosis (p = 0.003). Fair agreement was observed between in situ and invasive components with regard to grade (weighted kappa = 0.23).

Conclusions: The high nuclear grade and the presence of comedonecrosis were identified more frequently in younger patients and more often correlated with the solid pattern of DCIS.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_227.

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Ductal carcinoma in situ of the breast: different nuclear grades and comedonecrosis.A - Low grade ductal carcinoma in situ, cribriform type, showing uniform cells with mild atypia. Hematoxylin and eosin, x200. B – Intermediate grade ductal carcinoma in situ, showing cells with mild a moderate atypia and focal necrosis. Hematoxylin and eosin, x200. C – High grade ductal carcinoma in situ, solid type, showing severe atypical cells, without necrosis. Hematoxylin and eosin, x400. D – High grade ductal carcinoma in situ, solid type with extensive comedonecrosis. Hematoxylin and eosin, x200.
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Fig1: Ductal carcinoma in situ of the breast: different nuclear grades and comedonecrosis.A - Low grade ductal carcinoma in situ, cribriform type, showing uniform cells with mild atypia. Hematoxylin and eosin, x200. B – Intermediate grade ductal carcinoma in situ, showing cells with mild a moderate atypia and focal necrosis. Hematoxylin and eosin, x200. C – High grade ductal carcinoma in situ, solid type, showing severe atypical cells, without necrosis. Hematoxylin and eosin, x400. D – High grade ductal carcinoma in situ, solid type with extensive comedonecrosis. Hematoxylin and eosin, x200.

Mentions: The nuclear grade of DCIS was evaluated in 392/403 cases (97.3%; Fig. 1A-C). High-grade DCIS was identified in 293/403 cases (72.7%; Figure 1C). There was no significant difference in nuclear grade between pure DCIS and DCIS associated with invasive carcinoma (p = 0.142). The frequencies of different nuclear grades of DCIS and the relationship between nuclear grade and different single architectural patterns are respectively shown in Tables 2 and 3. Among DCIS with a single architectural pattern, high grade was more common in the solid subtype (151/168 cases, 89.9%; p < 0.001). Only 32% of tumors with a cribriform pattern had high nuclear grade.Figure 1


Ductal carcinoma in situ of the breast: correlation between histopathological features and age of patients.

Perez AA, Balabram D, Salles Mde A, Gobbi H - Diagn Pathol (2014)

Ductal carcinoma in situ of the breast: different nuclear grades and comedonecrosis.A - Low grade ductal carcinoma in situ, cribriform type, showing uniform cells with mild atypia. Hematoxylin and eosin, x200. B – Intermediate grade ductal carcinoma in situ, showing cells with mild a moderate atypia and focal necrosis. Hematoxylin and eosin, x200. C – High grade ductal carcinoma in situ, solid type, showing severe atypical cells, without necrosis. Hematoxylin and eosin, x400. D – High grade ductal carcinoma in situ, solid type with extensive comedonecrosis. Hematoxylin and eosin, x200.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4260240&req=5

Fig1: Ductal carcinoma in situ of the breast: different nuclear grades and comedonecrosis.A - Low grade ductal carcinoma in situ, cribriform type, showing uniform cells with mild atypia. Hematoxylin and eosin, x200. B – Intermediate grade ductal carcinoma in situ, showing cells with mild a moderate atypia and focal necrosis. Hematoxylin and eosin, x200. C – High grade ductal carcinoma in situ, solid type, showing severe atypical cells, without necrosis. Hematoxylin and eosin, x400. D – High grade ductal carcinoma in situ, solid type with extensive comedonecrosis. Hematoxylin and eosin, x200.
Mentions: The nuclear grade of DCIS was evaluated in 392/403 cases (97.3%; Fig. 1A-C). High-grade DCIS was identified in 293/403 cases (72.7%; Figure 1C). There was no significant difference in nuclear grade between pure DCIS and DCIS associated with invasive carcinoma (p = 0.142). The frequencies of different nuclear grades of DCIS and the relationship between nuclear grade and different single architectural patterns are respectively shown in Tables 2 and 3. Among DCIS with a single architectural pattern, high grade was more common in the solid subtype (151/168 cases, 89.9%; p < 0.001). Only 32% of tumors with a cribriform pattern had high nuclear grade.Figure 1

Bottom Line: Only 32% of tumours with a cribriform pattern had high nuclear grade.Comedonecrosis was more common in the solid morphology than in the cribriform, papillary and micropapillary subtypes (p < 0.001).Fair agreement was observed between in situ and invasive components with regard to grade (weighted kappa = 0.23).

View Article: PubMed Central - PubMed

Affiliation: Breast Pathology Laboratory, School of Medicine, Federal University of Minas Gerais, Av. Prof. Alfredo Balena, 190, Belo Horizonte, Minas Gerais, Brasil. amandaperez1408@yahoo.com.br.

ABSTRACT

Background: The histopathological subtype, nuclear grade and presence or absence of comedonecrosis are established as critical elements in the reporting of ductal carcinoma in situ (DCIS) of the breast. The aims of this study were to determine the frequencies of morphological subtypes of DCIS, nuclear grade and comedonecrosis; to compare the age of patients with the histopathological characteristics of DCIS, and to assess the agreement of grade between in situ and invasive components in DCIS cases that were associated with invasive carcinoma.

Methods: We evaluated a series of 403 cases of DCIS, pure or associated with invasive mammary carcinoma, consecutively identified from the histopathology files of the Breast Pathology Laboratory, Federal University of Minas Gerais, Brazil, from 2003 to 2008.

Results: DCIS displayed a single growth pattern in most cases (55.1%) and the solid subtype was the most common morphology (42.2% of the total). High-grade DCIS was identified in 293/403 cases (72.7%) and comedonecrosis was present in 222/403 cases (55%). Among DCIS with a single architectural pattern, high grade was more common in the solid subtype (151/168 cases, 89.9%; p < 0.001). Only 32% of tumours with a cribriform pattern had high nuclear grade. Comedonecrosis was more common in the solid morphology than in the cribriform, papillary and micropapillary subtypes (p < 0.001). Patients with high-grade DCIS were younger in relation to patients with low-grade DCIS (p = 0.027) and patients with tumours with comedonecrosis were also younger in comparison to patients with tumours without comedonecrosis (p = 0.003). Fair agreement was observed between in situ and invasive components with regard to grade (weighted kappa = 0.23).

Conclusions: The high nuclear grade and the presence of comedonecrosis were identified more frequently in younger patients and more often correlated with the solid pattern of DCIS.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_227.

Show MeSH
Related in: MedlinePlus