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Osteocompatibility of biofilm inhibitors.

Rawson M, Haggard W, Jennings JA - Open Orthop J (2014)

Bottom Line: Farnesol and dFBr induced cytotoxic responses within the reported biofilm inhibitory concentration range and low doses of dFBr were found to inhibit osteoblast differentiation.At high concentrations, such as those that may be present after local delivery, many of these biofilm inhibitors can have effects on cellular viability and osteoblast function.Concentrations at which negative effects on osteoblasts occur should serve as upper limits for delivery to orthopaedic trauma sites and guide development of these potential therapeutics for orthopaedics.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Engineering, University of Memphis, 330 Engineering Technology Building, Memphis, TN 38152, USA.

ABSTRACT
The demand for infection prevention therapies has led to the discovery of several biofilm inhibitors. These inhibiting signals are released by bacteria, fungi, or marine organisms to signal biofilm dispersal or disruption in Gram-positive, Gram-negative, and fungal microorganisms. The purpose of this study was to test the biocompatibility of five different naturally-produced biofilm chemical dispersal and inhibition signals with osteoblast-like cells: D-amino acids (D-AA), lysostaphin (LS), farnesol, cis-2-decenoic acid (C2DA), and desformyl flustrabromine (dFBr). In this preliminary study, compatibility of these anti-biofilm agents with differentiating osteoblasts was examined over a 21 days period at levels above and below concentrations active against bacterial biofilm. Anti-biofilm compounds listed above were serially diluted in osteogenic media and added to cultures of MC3T3 cells. Cell viability and cytotoxicity, after exposure to each anti-biofilm agent, were measured using a DNA assay. Differentiation characteristics of osteoblasts were determined qualitatively by observing staining of mineral deposits and quantitatively with an alkaline phosphatase assay. D-AA, LS, and C2DA were all biocompatible within the reported biofilm inhibitory concentration ranges and supported osteoblast differentiation. Farnesol and dFBr induced cytotoxic responses within the reported biofilm inhibitory concentration range and low doses of dFBr were found to inhibit osteoblast differentiation. At high concentrations, such as those that may be present after local delivery, many of these biofilm inhibitors can have effects on cellular viability and osteoblast function. Concentrations at which negative effects on osteoblasts occur should serve as upper limits for delivery to orthopaedic trauma sites and guide development of these potential therapeutics for orthopaedics.

No MeSH data available.


Related in: MedlinePlus

DNA quantity for a) all the control groups and b-f) biofilminhibitors to osteogenic controls on day 1 and 3 at varyingconcentrations. Data are represented as mean ± standard deviation;*indicates significant difference compared to osteogenic control.
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Figure 1: DNA quantity for a) all the control groups and b-f) biofilminhibitors to osteogenic controls on day 1 and 3 at varyingconcentrations. Data are represented as mean ± standard deviation;*indicates significant difference compared to osteogenic control.

Mentions: MC3T3 cells in osteogenic media had a positive growth rate until day 3, then cell viability remained steady over the following two weeks as cells became confluent (Fig. 1a).


Osteocompatibility of biofilm inhibitors.

Rawson M, Haggard W, Jennings JA - Open Orthop J (2014)

DNA quantity for a) all the control groups and b-f) biofilminhibitors to osteogenic controls on day 1 and 3 at varyingconcentrations. Data are represented as mean ± standard deviation;*indicates significant difference compared to osteogenic control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4260234&req=5

Figure 1: DNA quantity for a) all the control groups and b-f) biofilminhibitors to osteogenic controls on day 1 and 3 at varyingconcentrations. Data are represented as mean ± standard deviation;*indicates significant difference compared to osteogenic control.
Mentions: MC3T3 cells in osteogenic media had a positive growth rate until day 3, then cell viability remained steady over the following two weeks as cells became confluent (Fig. 1a).

Bottom Line: Farnesol and dFBr induced cytotoxic responses within the reported biofilm inhibitory concentration range and low doses of dFBr were found to inhibit osteoblast differentiation.At high concentrations, such as those that may be present after local delivery, many of these biofilm inhibitors can have effects on cellular viability and osteoblast function.Concentrations at which negative effects on osteoblasts occur should serve as upper limits for delivery to orthopaedic trauma sites and guide development of these potential therapeutics for orthopaedics.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Engineering, University of Memphis, 330 Engineering Technology Building, Memphis, TN 38152, USA.

ABSTRACT
The demand for infection prevention therapies has led to the discovery of several biofilm inhibitors. These inhibiting signals are released by bacteria, fungi, or marine organisms to signal biofilm dispersal or disruption in Gram-positive, Gram-negative, and fungal microorganisms. The purpose of this study was to test the biocompatibility of five different naturally-produced biofilm chemical dispersal and inhibition signals with osteoblast-like cells: D-amino acids (D-AA), lysostaphin (LS), farnesol, cis-2-decenoic acid (C2DA), and desformyl flustrabromine (dFBr). In this preliminary study, compatibility of these anti-biofilm agents with differentiating osteoblasts was examined over a 21 days period at levels above and below concentrations active against bacterial biofilm. Anti-biofilm compounds listed above were serially diluted in osteogenic media and added to cultures of MC3T3 cells. Cell viability and cytotoxicity, after exposure to each anti-biofilm agent, were measured using a DNA assay. Differentiation characteristics of osteoblasts were determined qualitatively by observing staining of mineral deposits and quantitatively with an alkaline phosphatase assay. D-AA, LS, and C2DA were all biocompatible within the reported biofilm inhibitory concentration ranges and supported osteoblast differentiation. Farnesol and dFBr induced cytotoxic responses within the reported biofilm inhibitory concentration range and low doses of dFBr were found to inhibit osteoblast differentiation. At high concentrations, such as those that may be present after local delivery, many of these biofilm inhibitors can have effects on cellular viability and osteoblast function. Concentrations at which negative effects on osteoblasts occur should serve as upper limits for delivery to orthopaedic trauma sites and guide development of these potential therapeutics for orthopaedics.

No MeSH data available.


Related in: MedlinePlus