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Ultrasensitive non-enzymatic glucose sensor based on three-dimensional network of ZnO-CuO hierarchical nanocomposites by electrospinning.

Zhou C, Xu L, Song J, Xing R, Xu S, Liu D, Song H - Sci Rep (2014)

Bottom Line: Three-dimensional (3D) porous ZnO-CuO hierarchical nanocomposites (HNCs) nonenzymatic glucose electrodes with different thicknesses were fabricated by coelectrospinning and compared with 3D mixed ZnO/CuO nanowires (NWs) and pure CuO NWs electrodes.Moreover, a good synergetic effect between CuO and ZnO was confirmed.The nonenzymatic biosensing properties of as prepared 3D porous electrodes based on fluorine doped tin oxide (FTO) were studied and the results indicated that the sensing properties of 3D porous ZnO-CuO HNCs electrodes were significantly improved and depended strongly on the thickness of the HNCs.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun, 130012, People's Republic of China.

ABSTRACT
Three-dimensional (3D) porous ZnO-CuO hierarchical nanocomposites (HNCs) nonenzymatic glucose electrodes with different thicknesses were fabricated by coelectrospinning and compared with 3D mixed ZnO/CuO nanowires (NWs) and pure CuO NWs electrodes. The structural characterization revealed that the ZnO-CuO HNCs were composed of the ZnO and CuO mixed NWs trunk (~200 nm), whose outer surface was attached with small CuO nanoparticles (NPs). Moreover, a good synergetic effect between CuO and ZnO was confirmed. The nonenzymatic biosensing properties of as prepared 3D porous electrodes based on fluorine doped tin oxide (FTO) were studied and the results indicated that the sensing properties of 3D porous ZnO-CuO HNCs electrodes were significantly improved and depended strongly on the thickness of the HNCs. At an applied potential of + 0.7 V, the optimum ZnO-CuO HNCs electrode presented a high sensitivity of 3066.4 μAmM(-1)cm(-2), the linear range up to 1.6 mM, and low practical detection limit of 0.21 μM. It also showed outstanding long term stability, good reproducibility, excellent selectivity and accurate measurement in real serum sample. The formation of special hierarchical heterojunction and the well-constructed 3D structure were the main reasons for the enhanced nonenzymatic biosensing behavior.

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The amperometric response of the 3D porous ZnO–CuO HNCs electrode (20 min) (a) with successive additions of different interfering species (UA, AA, DA, NADH, Mg2+, and Ca2+) and (b) with sequential addition of 10 μΜ various interfering sugars (maltose, mannose, lactose, and galactose) after initial addition of 100 μΜ glucose. The inset of (a) is its current response towards to 100 µM glucose in human serum.
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f9: The amperometric response of the 3D porous ZnO–CuO HNCs electrode (20 min) (a) with successive additions of different interfering species (UA, AA, DA, NADH, Mg2+, and Ca2+) and (b) with sequential addition of 10 μΜ various interfering sugars (maltose, mannose, lactose, and galactose) after initial addition of 100 μΜ glucose. The inset of (a) is its current response towards to 100 µM glucose in human serum.

Mentions: The electrochemical response of the interfering species of 3D porous ZnO–CuO HNCs electrode (20 min) was examined, as shown in Fig. 9. Although the concentration of interfering species in the human blood is 30 to 50 times less than that of glucose41, much higher concentrations of the interfering species (glucose: interfering species = 10:1) were used in the present analysis to ensure the sensitivity. First, some common interfering species in human serum, such as uric acid (UA), ascorbic acid (AA), dopamine acid (DA), nicotinamide adenine dinucleotide (NADH), Mg2+, and Ca2+, were chosen as the interfering species. As is clearly seen in Fig. 9a, the current responses of interfering species have little effect compared to glucose. In addition, other sugars, such as lactose, mannose, galactose, and maltose, are also present in abundant amounts in human blood, thus the selectivity to these organic compounds were also evaluated as shown in Fig. 9b. The current responses of the corresponding interfering species are also very low in the presence 100 μΜ of glucose. Here, the good selectivity of 3D porous ZnO–CuO HNCs electrode (20 min) against to some reducing compounds, such as AA, can be ascribed to the obvious repulsion of negatively charged CuO and ZnO (the isoelectric point both are about 9.5) and the negatively charged AA (deprotonated) under highly basic conditions74243. The good selectivity of 3D porous ZnO–CuO HNCs electrode (20 min) for glucose and against other interfering materials can be ascribed to the synergetic action of well-designed 3D porous structure, high surface area and HNCs structure, which provide maximum numbers of active free paths to the glucose molecules and facilitate faster electron transfer (electronic kinetics).


Ultrasensitive non-enzymatic glucose sensor based on three-dimensional network of ZnO-CuO hierarchical nanocomposites by electrospinning.

Zhou C, Xu L, Song J, Xing R, Xu S, Liu D, Song H - Sci Rep (2014)

The amperometric response of the 3D porous ZnO–CuO HNCs electrode (20 min) (a) with successive additions of different interfering species (UA, AA, DA, NADH, Mg2+, and Ca2+) and (b) with sequential addition of 10 μΜ various interfering sugars (maltose, mannose, lactose, and galactose) after initial addition of 100 μΜ glucose. The inset of (a) is its current response towards to 100 µM glucose in human serum.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4260231&req=5

f9: The amperometric response of the 3D porous ZnO–CuO HNCs electrode (20 min) (a) with successive additions of different interfering species (UA, AA, DA, NADH, Mg2+, and Ca2+) and (b) with sequential addition of 10 μΜ various interfering sugars (maltose, mannose, lactose, and galactose) after initial addition of 100 μΜ glucose. The inset of (a) is its current response towards to 100 µM glucose in human serum.
Mentions: The electrochemical response of the interfering species of 3D porous ZnO–CuO HNCs electrode (20 min) was examined, as shown in Fig. 9. Although the concentration of interfering species in the human blood is 30 to 50 times less than that of glucose41, much higher concentrations of the interfering species (glucose: interfering species = 10:1) were used in the present analysis to ensure the sensitivity. First, some common interfering species in human serum, such as uric acid (UA), ascorbic acid (AA), dopamine acid (DA), nicotinamide adenine dinucleotide (NADH), Mg2+, and Ca2+, were chosen as the interfering species. As is clearly seen in Fig. 9a, the current responses of interfering species have little effect compared to glucose. In addition, other sugars, such as lactose, mannose, galactose, and maltose, are also present in abundant amounts in human blood, thus the selectivity to these organic compounds were also evaluated as shown in Fig. 9b. The current responses of the corresponding interfering species are also very low in the presence 100 μΜ of glucose. Here, the good selectivity of 3D porous ZnO–CuO HNCs electrode (20 min) against to some reducing compounds, such as AA, can be ascribed to the obvious repulsion of negatively charged CuO and ZnO (the isoelectric point both are about 9.5) and the negatively charged AA (deprotonated) under highly basic conditions74243. The good selectivity of 3D porous ZnO–CuO HNCs electrode (20 min) for glucose and against other interfering materials can be ascribed to the synergetic action of well-designed 3D porous structure, high surface area and HNCs structure, which provide maximum numbers of active free paths to the glucose molecules and facilitate faster electron transfer (electronic kinetics).

Bottom Line: Three-dimensional (3D) porous ZnO-CuO hierarchical nanocomposites (HNCs) nonenzymatic glucose electrodes with different thicknesses were fabricated by coelectrospinning and compared with 3D mixed ZnO/CuO nanowires (NWs) and pure CuO NWs electrodes.Moreover, a good synergetic effect between CuO and ZnO was confirmed.The nonenzymatic biosensing properties of as prepared 3D porous electrodes based on fluorine doped tin oxide (FTO) were studied and the results indicated that the sensing properties of 3D porous ZnO-CuO HNCs electrodes were significantly improved and depended strongly on the thickness of the HNCs.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun, 130012, People's Republic of China.

ABSTRACT
Three-dimensional (3D) porous ZnO-CuO hierarchical nanocomposites (HNCs) nonenzymatic glucose electrodes with different thicknesses were fabricated by coelectrospinning and compared with 3D mixed ZnO/CuO nanowires (NWs) and pure CuO NWs electrodes. The structural characterization revealed that the ZnO-CuO HNCs were composed of the ZnO and CuO mixed NWs trunk (~200 nm), whose outer surface was attached with small CuO nanoparticles (NPs). Moreover, a good synergetic effect between CuO and ZnO was confirmed. The nonenzymatic biosensing properties of as prepared 3D porous electrodes based on fluorine doped tin oxide (FTO) were studied and the results indicated that the sensing properties of 3D porous ZnO-CuO HNCs electrodes were significantly improved and depended strongly on the thickness of the HNCs. At an applied potential of + 0.7 V, the optimum ZnO-CuO HNCs electrode presented a high sensitivity of 3066.4 μAmM(-1)cm(-2), the linear range up to 1.6 mM, and low practical detection limit of 0.21 μM. It also showed outstanding long term stability, good reproducibility, excellent selectivity and accurate measurement in real serum sample. The formation of special hierarchical heterojunction and the well-constructed 3D structure were the main reasons for the enhanced nonenzymatic biosensing behavior.

Show MeSH