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Role of dermatopontin in re-epithelialization: implications on keratinocyte migration and proliferation.

Krishnaswamy VR, Korrapati PS - Sci Rep (2014)

Bottom Line: Re-epithelialization is a key event in wound healing and any impairment in that process is associated with various pathological conditions.The results showed that DPT promotes keratinocyte migration in a dose dependant fashion but fail to induce proliferation.To conclude, DPT has a profound role in wound healing specifically during re-epithelialization by promoting keratinocyte migration via paracrine action from the underlying dermis.

View Article: PubMed Central - PubMed

Affiliation: Biomaterials Department, CSIR - Central Leather Research Institute, Adyar, Chennai - 600 020, Tamil Nadu, India.

ABSTRACT
Re-epithelialization is a key event in wound healing and any impairment in that process is associated with various pathological conditions. Epidermal keratinocyte migration and proliferation during re-epithelialization is largely regulated by the cytokines and growth factors from the provisional matrix and dermis. Extracellular matrix consists of numerous growth factors which mediate cell migration via cell membrane receptors. Dermatopontin (DPT), a non-collagenous matrix protein highly expressed in dermis is known for its striking ability to promote cell adhesion. DPT also enhances the biological activity of transforming growth factor beta 1 which plays a central role in the process of wound healing. This study was designed to envisage the role of DPT in keratinocyte migration and proliferation along with its mRNA and protein expression pattern in epidermis. The results showed that DPT promotes keratinocyte migration in a dose dependant fashion but fail to induce proliferation. Further, PCR and immunodetection studies revealed that the mRNA and protein expression of DPT is considerably negligible in the epidermis in contrast to the dermis. To conclude, DPT has a profound role in wound healing specifically during re-epithelialization by promoting keratinocyte migration via paracrine action from the underlying dermis.

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Related in: MedlinePlus

Effect of DPT on Keratinocyte growth.Graphical depiction of the proliferative potential of DPT on keratinocytes assessed through MTT assay. The values represent the mean of three repeated experiments with triplicates for each concentration.
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f5: Effect of DPT on Keratinocyte growth.Graphical depiction of the proliferative potential of DPT on keratinocytes assessed through MTT assay. The values represent the mean of three repeated experiments with triplicates for each concentration.

Mentions: The results of MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay is illustrated in fig. 5. The intensity of the color measured in control and treated cells was notably unaltered indicating that DPT has no effect on the proliferation of HaCaT cells. In spite of the increase in the concentration (up to 100 ng/mL) and the treatment period (up to 72 hours) no substantial change was observed confirming that DPT has no potential role in keratinocyte proliferation.


Role of dermatopontin in re-epithelialization: implications on keratinocyte migration and proliferation.

Krishnaswamy VR, Korrapati PS - Sci Rep (2014)

Effect of DPT on Keratinocyte growth.Graphical depiction of the proliferative potential of DPT on keratinocytes assessed through MTT assay. The values represent the mean of three repeated experiments with triplicates for each concentration.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4260223&req=5

f5: Effect of DPT on Keratinocyte growth.Graphical depiction of the proliferative potential of DPT on keratinocytes assessed through MTT assay. The values represent the mean of three repeated experiments with triplicates for each concentration.
Mentions: The results of MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay is illustrated in fig. 5. The intensity of the color measured in control and treated cells was notably unaltered indicating that DPT has no effect on the proliferation of HaCaT cells. In spite of the increase in the concentration (up to 100 ng/mL) and the treatment period (up to 72 hours) no substantial change was observed confirming that DPT has no potential role in keratinocyte proliferation.

Bottom Line: Re-epithelialization is a key event in wound healing and any impairment in that process is associated with various pathological conditions.The results showed that DPT promotes keratinocyte migration in a dose dependant fashion but fail to induce proliferation.To conclude, DPT has a profound role in wound healing specifically during re-epithelialization by promoting keratinocyte migration via paracrine action from the underlying dermis.

View Article: PubMed Central - PubMed

Affiliation: Biomaterials Department, CSIR - Central Leather Research Institute, Adyar, Chennai - 600 020, Tamil Nadu, India.

ABSTRACT
Re-epithelialization is a key event in wound healing and any impairment in that process is associated with various pathological conditions. Epidermal keratinocyte migration and proliferation during re-epithelialization is largely regulated by the cytokines and growth factors from the provisional matrix and dermis. Extracellular matrix consists of numerous growth factors which mediate cell migration via cell membrane receptors. Dermatopontin (DPT), a non-collagenous matrix protein highly expressed in dermis is known for its striking ability to promote cell adhesion. DPT also enhances the biological activity of transforming growth factor beta 1 which plays a central role in the process of wound healing. This study was designed to envisage the role of DPT in keratinocyte migration and proliferation along with its mRNA and protein expression pattern in epidermis. The results showed that DPT promotes keratinocyte migration in a dose dependant fashion but fail to induce proliferation. Further, PCR and immunodetection studies revealed that the mRNA and protein expression of DPT is considerably negligible in the epidermis in contrast to the dermis. To conclude, DPT has a profound role in wound healing specifically during re-epithelialization by promoting keratinocyte migration via paracrine action from the underlying dermis.

Show MeSH
Related in: MedlinePlus