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Role of dermatopontin in re-epithelialization: implications on keratinocyte migration and proliferation.

Krishnaswamy VR, Korrapati PS - Sci Rep (2014)

Bottom Line: Re-epithelialization is a key event in wound healing and any impairment in that process is associated with various pathological conditions.The results showed that DPT promotes keratinocyte migration in a dose dependant fashion but fail to induce proliferation.To conclude, DPT has a profound role in wound healing specifically during re-epithelialization by promoting keratinocyte migration via paracrine action from the underlying dermis.

View Article: PubMed Central - PubMed

Affiliation: Biomaterials Department, CSIR - Central Leather Research Institute, Adyar, Chennai - 600 020, Tamil Nadu, India.

ABSTRACT
Re-epithelialization is a key event in wound healing and any impairment in that process is associated with various pathological conditions. Epidermal keratinocyte migration and proliferation during re-epithelialization is largely regulated by the cytokines and growth factors from the provisional matrix and dermis. Extracellular matrix consists of numerous growth factors which mediate cell migration via cell membrane receptors. Dermatopontin (DPT), a non-collagenous matrix protein highly expressed in dermis is known for its striking ability to promote cell adhesion. DPT also enhances the biological activity of transforming growth factor beta 1 which plays a central role in the process of wound healing. This study was designed to envisage the role of DPT in keratinocyte migration and proliferation along with its mRNA and protein expression pattern in epidermis. The results showed that DPT promotes keratinocyte migration in a dose dependant fashion but fail to induce proliferation. Further, PCR and immunodetection studies revealed that the mRNA and protein expression of DPT is considerably negligible in the epidermis in contrast to the dermis. To conclude, DPT has a profound role in wound healing specifically during re-epithelialization by promoting keratinocyte migration via paracrine action from the underlying dermis.

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Related in: MedlinePlus

Effect of DPT on keratinocytes migration.Graphical illustration of the percentage of wound area recovered with various concentrations of DPT treatment. ** P-Value (0.0258) was calculated using student's t-test for the indicated concentration by comparing with the untreated cells at the same time point.
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f1: Effect of DPT on keratinocytes migration.Graphical illustration of the percentage of wound area recovered with various concentrations of DPT treatment. ** P-Value (0.0258) was calculated using student's t-test for the indicated concentration by comparing with the untreated cells at the same time point.

Mentions: The migratory potential of DPT on adhered keratinocytes was assessed using standard scratch wound assay. The wounded keratinocyte monolayer when treated with various concentrations (50–500 pg/mL) of DPT showed a dose dependant increase in the migration (fig. 1 and fig. 2). The percentage of wound area recovered after 8 hours in DPT treated and untreated cells were 20.05 and 10.47 respectively. The recovery of wound as a measure of cell migration was twice in treated cells when compared to the untreated cells indicating that DPT significantly influences keratinocyte cell migration.


Role of dermatopontin in re-epithelialization: implications on keratinocyte migration and proliferation.

Krishnaswamy VR, Korrapati PS - Sci Rep (2014)

Effect of DPT on keratinocytes migration.Graphical illustration of the percentage of wound area recovered with various concentrations of DPT treatment. ** P-Value (0.0258) was calculated using student's t-test for the indicated concentration by comparing with the untreated cells at the same time point.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4260223&req=5

f1: Effect of DPT on keratinocytes migration.Graphical illustration of the percentage of wound area recovered with various concentrations of DPT treatment. ** P-Value (0.0258) was calculated using student's t-test for the indicated concentration by comparing with the untreated cells at the same time point.
Mentions: The migratory potential of DPT on adhered keratinocytes was assessed using standard scratch wound assay. The wounded keratinocyte monolayer when treated with various concentrations (50–500 pg/mL) of DPT showed a dose dependant increase in the migration (fig. 1 and fig. 2). The percentage of wound area recovered after 8 hours in DPT treated and untreated cells were 20.05 and 10.47 respectively. The recovery of wound as a measure of cell migration was twice in treated cells when compared to the untreated cells indicating that DPT significantly influences keratinocyte cell migration.

Bottom Line: Re-epithelialization is a key event in wound healing and any impairment in that process is associated with various pathological conditions.The results showed that DPT promotes keratinocyte migration in a dose dependant fashion but fail to induce proliferation.To conclude, DPT has a profound role in wound healing specifically during re-epithelialization by promoting keratinocyte migration via paracrine action from the underlying dermis.

View Article: PubMed Central - PubMed

Affiliation: Biomaterials Department, CSIR - Central Leather Research Institute, Adyar, Chennai - 600 020, Tamil Nadu, India.

ABSTRACT
Re-epithelialization is a key event in wound healing and any impairment in that process is associated with various pathological conditions. Epidermal keratinocyte migration and proliferation during re-epithelialization is largely regulated by the cytokines and growth factors from the provisional matrix and dermis. Extracellular matrix consists of numerous growth factors which mediate cell migration via cell membrane receptors. Dermatopontin (DPT), a non-collagenous matrix protein highly expressed in dermis is known for its striking ability to promote cell adhesion. DPT also enhances the biological activity of transforming growth factor beta 1 which plays a central role in the process of wound healing. This study was designed to envisage the role of DPT in keratinocyte migration and proliferation along with its mRNA and protein expression pattern in epidermis. The results showed that DPT promotes keratinocyte migration in a dose dependant fashion but fail to induce proliferation. Further, PCR and immunodetection studies revealed that the mRNA and protein expression of DPT is considerably negligible in the epidermis in contrast to the dermis. To conclude, DPT has a profound role in wound healing specifically during re-epithelialization by promoting keratinocyte migration via paracrine action from the underlying dermis.

Show MeSH
Related in: MedlinePlus