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Novel metal allergy patch test using metal nanoballs.

Sugiyama T, Uo M, Wada T, Hongo T, Omagari D, Komiyama K, Sasaki H, Takahashi H, Kusama M, Mori Y - J Nanobiotechnology (2014)

Bottom Line: Consequently, in the new test system, reactions caused by high concentrations of metal salts were avoided.By exploiting the high specific surface area of Ni nanoballs, we obtained an effective dissolution of Ni ions that triggered Ni allergy in the absence of direct contact between the nanoballs and mouse skin.This novel patch system can be applied to other metals and alloys for diagnosing various types of metal-induced contact dermatitis.

View Article: PubMed Central - PubMed

Affiliation: Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan. tomoko_s@jichi.ac.jp.

ABSTRACT

Background: Patch tests are often used in the clinical diagnosis of metal allergies. In currently available patch tests, high concentrations of metal salt solutions are used. However, diagnosis accuracy can be influenced not only by acute skin reactions to high concentrations of metal salt, but also by skin reactions to other components present in the patch or to pH changes. In this study, we developed Ni nanoparticles (termed "nanoballs") for use in patch-test solutions.

Findings: Highly soluble, spherical Ni nanoballs were prepared using plasma electrolysis. The Ni released from the nanoballs permeated through a dialysis membrane, and the nanoball-containing solution's pH was maintained constant. Ni ions were released slowly at low concentrations in a time-dependent manner, which contrasted the rapid release observed in the case of a commercial patch test. Consequently, in the new test system, reactions caused by high concentrations of metal salts were avoided.

Conclusions: By exploiting the high specific surface area of Ni nanoballs, we obtained an effective dissolution of Ni ions that triggered Ni allergy in the absence of direct contact between the nanoballs and mouse skin. This novel patch system can be applied to other metals and alloys for diagnosing various types of metal-induced contact dermatitis.

No MeSH data available.


Related in: MedlinePlus

In vivoNi permeation from Ni-nanoball packs into mouse skin after 24-h application. Experimental setup (a), histopathological (H-E) images and elemental-distribution images of skin cross-sections obtained using SR-XRF analysis (b), and detailed elemental-distribution images obtained using micro-PIXE analysis (c).
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Fig3: In vivoNi permeation from Ni-nanoball packs into mouse skin after 24-h application. Experimental setup (a), histopathological (H-E) images and elemental-distribution images of skin cross-sections obtained using SR-XRF analysis (b), and detailed elemental-distribution images obtained using micro-PIXE analysis (c).

Mentions: Figure 3b shows H-E-stained cross-sections of mouse skin (histopathological analysis) and their SR-XRF images of elemental distribution obtained after treatment with the Ni-nanoball pack for 24 h. The Ni that permeated from the patch was clearly observed on the surface side of the skin. Figure 3c shows the detailed elemental distribution of S, P, and Ni in the areas that exhibited high Ni accumulation (white squares in Figure 3b), as assessed using micro-PIXE analysis. The Ni concentration was high in the epidermis and spread into the dermis layer beyond the basal layer. These results indicate a clear internal permeation of Ni. Furthermore, the H-E-stained images of the same area showed a slight inflammatory response at the epidermis. The regions of inflammation overlapped with localized areas of Ni and P accumulation. Because the phosphate originates from inflammatory cells, this colocalization of Ni and P suggests that the Ni that permeates from the patch and penetrates the skin induces local inflammation.Figure 3


Novel metal allergy patch test using metal nanoballs.

Sugiyama T, Uo M, Wada T, Hongo T, Omagari D, Komiyama K, Sasaki H, Takahashi H, Kusama M, Mori Y - J Nanobiotechnology (2014)

In vivoNi permeation from Ni-nanoball packs into mouse skin after 24-h application. Experimental setup (a), histopathological (H-E) images and elemental-distribution images of skin cross-sections obtained using SR-XRF analysis (b), and detailed elemental-distribution images obtained using micro-PIXE analysis (c).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4260209&req=5

Fig3: In vivoNi permeation from Ni-nanoball packs into mouse skin after 24-h application. Experimental setup (a), histopathological (H-E) images and elemental-distribution images of skin cross-sections obtained using SR-XRF analysis (b), and detailed elemental-distribution images obtained using micro-PIXE analysis (c).
Mentions: Figure 3b shows H-E-stained cross-sections of mouse skin (histopathological analysis) and their SR-XRF images of elemental distribution obtained after treatment with the Ni-nanoball pack for 24 h. The Ni that permeated from the patch was clearly observed on the surface side of the skin. Figure 3c shows the detailed elemental distribution of S, P, and Ni in the areas that exhibited high Ni accumulation (white squares in Figure 3b), as assessed using micro-PIXE analysis. The Ni concentration was high in the epidermis and spread into the dermis layer beyond the basal layer. These results indicate a clear internal permeation of Ni. Furthermore, the H-E-stained images of the same area showed a slight inflammatory response at the epidermis. The regions of inflammation overlapped with localized areas of Ni and P accumulation. Because the phosphate originates from inflammatory cells, this colocalization of Ni and P suggests that the Ni that permeates from the patch and penetrates the skin induces local inflammation.Figure 3

Bottom Line: Consequently, in the new test system, reactions caused by high concentrations of metal salts were avoided.By exploiting the high specific surface area of Ni nanoballs, we obtained an effective dissolution of Ni ions that triggered Ni allergy in the absence of direct contact between the nanoballs and mouse skin.This novel patch system can be applied to other metals and alloys for diagnosing various types of metal-induced contact dermatitis.

View Article: PubMed Central - PubMed

Affiliation: Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan. tomoko_s@jichi.ac.jp.

ABSTRACT

Background: Patch tests are often used in the clinical diagnosis of metal allergies. In currently available patch tests, high concentrations of metal salt solutions are used. However, diagnosis accuracy can be influenced not only by acute skin reactions to high concentrations of metal salt, but also by skin reactions to other components present in the patch or to pH changes. In this study, we developed Ni nanoparticles (termed "nanoballs") for use in patch-test solutions.

Findings: Highly soluble, spherical Ni nanoballs were prepared using plasma electrolysis. The Ni released from the nanoballs permeated through a dialysis membrane, and the nanoball-containing solution's pH was maintained constant. Ni ions were released slowly at low concentrations in a time-dependent manner, which contrasted the rapid release observed in the case of a commercial patch test. Consequently, in the new test system, reactions caused by high concentrations of metal salts were avoided.

Conclusions: By exploiting the high specific surface area of Ni nanoballs, we obtained an effective dissolution of Ni ions that triggered Ni allergy in the absence of direct contact between the nanoballs and mouse skin. This novel patch system can be applied to other metals and alloys for diagnosing various types of metal-induced contact dermatitis.

No MeSH data available.


Related in: MedlinePlus