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Novel metal allergy patch test using metal nanoballs.

Sugiyama T, Uo M, Wada T, Hongo T, Omagari D, Komiyama K, Sasaki H, Takahashi H, Kusama M, Mori Y - J Nanobiotechnology (2014)

Bottom Line: Consequently, in the new test system, reactions caused by high concentrations of metal salts were avoided.By exploiting the high specific surface area of Ni nanoballs, we obtained an effective dissolution of Ni ions that triggered Ni allergy in the absence of direct contact between the nanoballs and mouse skin.This novel patch system can be applied to other metals and alloys for diagnosing various types of metal-induced contact dermatitis.

View Article: PubMed Central - PubMed

Affiliation: Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan. tomoko_s@jichi.ac.jp.

ABSTRACT

Background: Patch tests are often used in the clinical diagnosis of metal allergies. In currently available patch tests, high concentrations of metal salt solutions are used. However, diagnosis accuracy can be influenced not only by acute skin reactions to high concentrations of metal salt, but also by skin reactions to other components present in the patch or to pH changes. In this study, we developed Ni nanoparticles (termed "nanoballs") for use in patch-test solutions.

Findings: Highly soluble, spherical Ni nanoballs were prepared using plasma electrolysis. The Ni released from the nanoballs permeated through a dialysis membrane, and the nanoball-containing solution's pH was maintained constant. Ni ions were released slowly at low concentrations in a time-dependent manner, which contrasted the rapid release observed in the case of a commercial patch test. Consequently, in the new test system, reactions caused by high concentrations of metal salts were avoided.

Conclusions: By exploiting the high specific surface area of Ni nanoballs, we obtained an effective dissolution of Ni ions that triggered Ni allergy in the absence of direct contact between the nanoballs and mouse skin. This novel patch system can be applied to other metals and alloys for diagnosing various types of metal-induced contact dermatitis.

No MeSH data available.


Related in: MedlinePlus

Various patch-test schemes. A currently available commercial patch test (a), an ideal patch test (b), and the novel patch test designed using Ni nanoballs (c).
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Fig1: Various patch-test schemes. A currently available commercial patch test (a), an ideal patch test (b), and the novel patch test designed using Ni nanoballs (c).

Mentions: Metal-allergy patch tests are routinely used in the clinical diagnosis of metal-induced contact dermatitis. Currently available patch tests use high concentrations of various metal salts in aqueous solution. They contain a reservoir sheet that allows the test solution to permeate into the skin to induce a local allergic reaction (FigureĀ 1a). A patch test solution typically contains a metal salt under an acidic condition [1]. Current patch tests can cause pustular or follicular reactions because of the high concentration of metal salts. In addition, false positive or negative reactions [2] and skin irritation often occur [3-5]. Metal allergies, however, are often triggered by metal ions that are continuously eroded from metallic materials under neutral pH conditions, and in this case, the metal ion concentration is typically low. Factors such as pH, metal-ion concentration, and dosage rates differ considerably between a genuine metal-allergic reaction and that which occurs in a patch test.Figure 1


Novel metal allergy patch test using metal nanoballs.

Sugiyama T, Uo M, Wada T, Hongo T, Omagari D, Komiyama K, Sasaki H, Takahashi H, Kusama M, Mori Y - J Nanobiotechnology (2014)

Various patch-test schemes. A currently available commercial patch test (a), an ideal patch test (b), and the novel patch test designed using Ni nanoballs (c).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4260209&req=5

Fig1: Various patch-test schemes. A currently available commercial patch test (a), an ideal patch test (b), and the novel patch test designed using Ni nanoballs (c).
Mentions: Metal-allergy patch tests are routinely used in the clinical diagnosis of metal-induced contact dermatitis. Currently available patch tests use high concentrations of various metal salts in aqueous solution. They contain a reservoir sheet that allows the test solution to permeate into the skin to induce a local allergic reaction (FigureĀ 1a). A patch test solution typically contains a metal salt under an acidic condition [1]. Current patch tests can cause pustular or follicular reactions because of the high concentration of metal salts. In addition, false positive or negative reactions [2] and skin irritation often occur [3-5]. Metal allergies, however, are often triggered by metal ions that are continuously eroded from metallic materials under neutral pH conditions, and in this case, the metal ion concentration is typically low. Factors such as pH, metal-ion concentration, and dosage rates differ considerably between a genuine metal-allergic reaction and that which occurs in a patch test.Figure 1

Bottom Line: Consequently, in the new test system, reactions caused by high concentrations of metal salts were avoided.By exploiting the high specific surface area of Ni nanoballs, we obtained an effective dissolution of Ni ions that triggered Ni allergy in the absence of direct contact between the nanoballs and mouse skin.This novel patch system can be applied to other metals and alloys for diagnosing various types of metal-induced contact dermatitis.

View Article: PubMed Central - PubMed

Affiliation: Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan. tomoko_s@jichi.ac.jp.

ABSTRACT

Background: Patch tests are often used in the clinical diagnosis of metal allergies. In currently available patch tests, high concentrations of metal salt solutions are used. However, diagnosis accuracy can be influenced not only by acute skin reactions to high concentrations of metal salt, but also by skin reactions to other components present in the patch or to pH changes. In this study, we developed Ni nanoparticles (termed "nanoballs") for use in patch-test solutions.

Findings: Highly soluble, spherical Ni nanoballs were prepared using plasma electrolysis. The Ni released from the nanoballs permeated through a dialysis membrane, and the nanoball-containing solution's pH was maintained constant. Ni ions were released slowly at low concentrations in a time-dependent manner, which contrasted the rapid release observed in the case of a commercial patch test. Consequently, in the new test system, reactions caused by high concentrations of metal salts were avoided.

Conclusions: By exploiting the high specific surface area of Ni nanoballs, we obtained an effective dissolution of Ni ions that triggered Ni allergy in the absence of direct contact between the nanoballs and mouse skin. This novel patch system can be applied to other metals and alloys for diagnosing various types of metal-induced contact dermatitis.

No MeSH data available.


Related in: MedlinePlus