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Neuropathological microscopic features of abortions induced by Bunyavirus / or Flavivirus infections.

Javanbakht J, Mardjanmehr SH, Tavasoly A, Nazemshirazi MH - Diagn Pathol (2014)

Bottom Line: Furthermore, microscopic lesions are mostly linked to a neurodegenerative and necrotic cell death process in the gray matter of ventral horn of the spinal cord.Briefly, histopathologic findings in the brain and spinal cord included hyperemia, hemorrhage, non-suppurative encephalitis, mononuclear perivascular cuffing, multifocal gliosis, cavitation, central chromatolysis, neuronal degeneration and necrosis, perineuronal and perivascular edema in the all regions of the brain and acute neuronal necrosis in the gray matter of ventral horn of the spinal cord were also seen.Our study suggested that the sheep fetuses are fully susceptible to viral infections and may even develop neurolopathological lesions upon natural infection with mentioned pathogens .Therefore ,according to,specific lesions caused by viral infections, we believe that the histopathological pattern were detected in this study could be associated with either viral infection and or mainly by a Bunyavirus / or Flavivirus strains that extensively shares common lesions with Rift Valley fever ,Wesselsbron ,Cache valley virus / or and Akabaneviruses.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran. javadjavanbakht@ut.ac.ir.

ABSTRACT

Background: The present study describes the pathologic changes in the brain and the spinal cord of aborted, stillbirth and deformities of newborn lambs infected with viral agents.

Methods: From February 2012 to March 2013, a total of 650 aborted fetuses from 793 pregnant ewes were studied from 8 flocks at different areas in the Mazandaran province in the north of Iran. And randomly, systematic necropsy was performed to collect tissues, and all gross abnormalities were recorded at necropsy by the pathologist .Nevertheless, we conducted a limited number of necropsies for aborted fetuses.

Results: In the most cases, arthrogryposis was the most common musculoskeletal defects and at necropsy, malformations of the brain included hydranencephaly, porencephaly, hydrocephalus and cerebellar hypoplasia, mainly in the brain stem and gray and white matter of the brain and cerebellum were observed. Histopathologic lesions included chronic multifocal lymphoplasmacytic encephalitis(nonsuppurative) with extensive perivascular cuffing in some cases, formation of glial nodules mainly in the mesencephalon, thalamus, hippocampus, pons and medulla oblongata in the brain of aborted fetuses, and neuronal degeneration, necrosis and central chromatolysis mainly in the cortex and subcortical of the brain and brain stem regions of them. Furthermore, microscopic lesions are mostly linked to a neurodegenerative and necrotic cell death process in the gray matter of ventral horn of the spinal cord. Briefly, histopathologic findings in the brain and spinal cord included hyperemia, hemorrhage, non-suppurative encephalitis, mononuclear perivascular cuffing, multifocal gliosis, cavitation, central chromatolysis, neuronal degeneration and necrosis, perineuronal and perivascular edema in the all regions of the brain and acute neuronal necrosis in the gray matter of ventral horn of the spinal cord were also seen.

Conclusion: Our study suggested that the sheep fetuses are fully susceptible to viral infections and may even develop neurolopathological lesions upon natural infection with mentioned pathogens .Therefore ,according to,specific lesions caused by viral infections, we believe that the histopathological pattern were detected in this study could be associated with either viral infection and or mainly by a Bunyavirus / or Flavivirus strains that extensively shares common lesions with Rift Valley fever ,Wesselsbron ,Cache valley virus / or and Akabaneviruses.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_223.

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Related in: MedlinePlus

Photomicrographs of a section of the brain from an ovine fetus; There is severe multifocal gliosis and a focus of gliosis and increased cellularity; H&E .200x and 400x. Bar =100 and 10 μm.
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Fig6: Photomicrographs of a section of the brain from an ovine fetus; There is severe multifocal gliosis and a focus of gliosis and increased cellularity; H&E .200x and 400x. Bar =100 and 10 μm.

Mentions: Microscopically, inflammation in the CNS characterized by a lymphoplasmacytic, mainly perivascular, infiltration of the gray and white matter in most investigated brain areas. Perivascular cuffs ranged from multifarious layers of mononuclear cells. In parallel, encephalitic changes were detected in most aborted fetuses. In these cases, a mild to moderate non-suppurative encephalitis, characterized by foci of perivascular cuffing with mononuclear cells, predominantly lymphocytes, was observed (Figures 4 and 5). The perivascular cuffing was generally associated with a mild to moderate gliosis (focal or diffuse), these changes were most prominent in the cranial brain regions and occasionally the caudal brain regions (pons and medulla). On the other hand, glial nodules (Figure 6) formed predominantly in the mesencephalon, thalamus, hippocampus, pons and medulla oblongata. Nevertheless, mild-to-moderate focal-to-multifocal gliosis associated with acute neuronal necrosis was observed for most cases (Figure 6). Additionally, Virchow–Robin spaces were expanded by cuffs of lymphocytes and plasma cells, admixed with fewer histiocytes and neutrophils. Moreover, multifocal hemorrhages were seen in several cases. These lesions varied in age within each case and between cases.Figure 4


Neuropathological microscopic features of abortions induced by Bunyavirus / or Flavivirus infections.

Javanbakht J, Mardjanmehr SH, Tavasoly A, Nazemshirazi MH - Diagn Pathol (2014)

Photomicrographs of a section of the brain from an ovine fetus; There is severe multifocal gliosis and a focus of gliosis and increased cellularity; H&E .200x and 400x. Bar =100 and 10 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4260183&req=5

Fig6: Photomicrographs of a section of the brain from an ovine fetus; There is severe multifocal gliosis and a focus of gliosis and increased cellularity; H&E .200x and 400x. Bar =100 and 10 μm.
Mentions: Microscopically, inflammation in the CNS characterized by a lymphoplasmacytic, mainly perivascular, infiltration of the gray and white matter in most investigated brain areas. Perivascular cuffs ranged from multifarious layers of mononuclear cells. In parallel, encephalitic changes were detected in most aborted fetuses. In these cases, a mild to moderate non-suppurative encephalitis, characterized by foci of perivascular cuffing with mononuclear cells, predominantly lymphocytes, was observed (Figures 4 and 5). The perivascular cuffing was generally associated with a mild to moderate gliosis (focal or diffuse), these changes were most prominent in the cranial brain regions and occasionally the caudal brain regions (pons and medulla). On the other hand, glial nodules (Figure 6) formed predominantly in the mesencephalon, thalamus, hippocampus, pons and medulla oblongata. Nevertheless, mild-to-moderate focal-to-multifocal gliosis associated with acute neuronal necrosis was observed for most cases (Figure 6). Additionally, Virchow–Robin spaces were expanded by cuffs of lymphocytes and plasma cells, admixed with fewer histiocytes and neutrophils. Moreover, multifocal hemorrhages were seen in several cases. These lesions varied in age within each case and between cases.Figure 4

Bottom Line: Furthermore, microscopic lesions are mostly linked to a neurodegenerative and necrotic cell death process in the gray matter of ventral horn of the spinal cord.Briefly, histopathologic findings in the brain and spinal cord included hyperemia, hemorrhage, non-suppurative encephalitis, mononuclear perivascular cuffing, multifocal gliosis, cavitation, central chromatolysis, neuronal degeneration and necrosis, perineuronal and perivascular edema in the all regions of the brain and acute neuronal necrosis in the gray matter of ventral horn of the spinal cord were also seen.Our study suggested that the sheep fetuses are fully susceptible to viral infections and may even develop neurolopathological lesions upon natural infection with mentioned pathogens .Therefore ,according to,specific lesions caused by viral infections, we believe that the histopathological pattern were detected in this study could be associated with either viral infection and or mainly by a Bunyavirus / or Flavivirus strains that extensively shares common lesions with Rift Valley fever ,Wesselsbron ,Cache valley virus / or and Akabaneviruses.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran. javadjavanbakht@ut.ac.ir.

ABSTRACT

Background: The present study describes the pathologic changes in the brain and the spinal cord of aborted, stillbirth and deformities of newborn lambs infected with viral agents.

Methods: From February 2012 to March 2013, a total of 650 aborted fetuses from 793 pregnant ewes were studied from 8 flocks at different areas in the Mazandaran province in the north of Iran. And randomly, systematic necropsy was performed to collect tissues, and all gross abnormalities were recorded at necropsy by the pathologist .Nevertheless, we conducted a limited number of necropsies for aborted fetuses.

Results: In the most cases, arthrogryposis was the most common musculoskeletal defects and at necropsy, malformations of the brain included hydranencephaly, porencephaly, hydrocephalus and cerebellar hypoplasia, mainly in the brain stem and gray and white matter of the brain and cerebellum were observed. Histopathologic lesions included chronic multifocal lymphoplasmacytic encephalitis(nonsuppurative) with extensive perivascular cuffing in some cases, formation of glial nodules mainly in the mesencephalon, thalamus, hippocampus, pons and medulla oblongata in the brain of aborted fetuses, and neuronal degeneration, necrosis and central chromatolysis mainly in the cortex and subcortical of the brain and brain stem regions of them. Furthermore, microscopic lesions are mostly linked to a neurodegenerative and necrotic cell death process in the gray matter of ventral horn of the spinal cord. Briefly, histopathologic findings in the brain and spinal cord included hyperemia, hemorrhage, non-suppurative encephalitis, mononuclear perivascular cuffing, multifocal gliosis, cavitation, central chromatolysis, neuronal degeneration and necrosis, perineuronal and perivascular edema in the all regions of the brain and acute neuronal necrosis in the gray matter of ventral horn of the spinal cord were also seen.

Conclusion: Our study suggested that the sheep fetuses are fully susceptible to viral infections and may even develop neurolopathological lesions upon natural infection with mentioned pathogens .Therefore ,according to,specific lesions caused by viral infections, we believe that the histopathological pattern were detected in this study could be associated with either viral infection and or mainly by a Bunyavirus / or Flavivirus strains that extensively shares common lesions with Rift Valley fever ,Wesselsbron ,Cache valley virus / or and Akabaneviruses.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_223.

Show MeSH
Related in: MedlinePlus