Characterization of a population of neural progenitor cells in the infant hippocampus.
Bottom Line: Previous work suggested that these cells were microglia and that their presence was associated with chronic illness and sudden infant death syndrome.The rod cells were consistently negative for the microglial markers CD45, CD68 and HLA-DR.These findings advance our understanding of postnatal neurogenesis in the human hippocampus in health and disease and are of diagnostic importance, allowing reactive microglia to be distinguished from the normal population of neural progenitors.
Affiliation: Neural Development Unit, Birth Defects Research Centre, UCL Institute of Child Health, London, UK; Department of Histopathology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, UK.Show MeSH
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Mentions: In all cases, rod cells were immunoreactive for doublecortin, nestin and TUJ1(Figure 2). The proportions of cells that werestained by nestin and TUJ1 was assessed qualitatively and found to be between 10% and30% and 60% and 80% respectively. Quantitative assessment of DCX expressionshowed that in the superior and inferior limbs 13.2% [95% confidence interval(CI) 10.7–15.7%] and 12.5% (95% CI 9.8–15.2%) ofrod cells were positive respectively. Occasional rod cells were positive for the proliferationmarker Ki67 (Supplementary Figure S2). The rodcells were uniformly negative for the stem cell markers CD133 and Oct3/4 (Supplementary Figure S2). In two offive cases stained, cytoplasmic immunoreactivity to SOX2 was noted in rare rod cells but nuclearstaining was not present (Supplementary Figure S2).
Affiliation: Neural Development Unit, Birth Defects Research Centre, UCL Institute of Child Health, London, UK; Department of Histopathology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, UK.