Carbohydrate recognition in the immune system: contributions of neoglycolipid-based microarrays to carbohydrate ligand discovery.
Bottom Line: The pinpointing and characterizing of oligosaccharide ligands within glycomes has been one of the most challenging aspects of molecular cell biology, as oligosaccharides cannot be cloned and are generally available in limited amounts.This overview recounts the background to the development of a microarray system that is poised for surveying proteomes for carbohydrate-binding activities and glycomes for assigning the oligosaccharide ligands.Particularly highlighted are sulfo-oligosaccharide and gluco-oligosaccharide recognition systems elucidated using microarrays.
Affiliation: The Glycosciences Laboratory, Department of Medicine, Imperial College London, London, United Kingdom. firstname.lastname@example.orgShow MeSH
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Mentions: In 2002, we introduced an oligosaccharide microarray system based on the NGL technology.44 Following development, this is now a state-of-the-art system (Fig. 2).42,45,46 Our library of sequence-defined probes currently numbers ∼800, and encompasses a variety of mammalian type sequences, representative of N-glycans (high-mannose-type and neutral and sialylated complex-type), peripheral regions of O-glycans; blood group antigen-related sequences (A, B, H, Lewisa, Lewisb, Lewisx, and Lewisy) on linear or branched backbones and their sialylated and/or sulfated analogs; linear and branched poly-N-acetyllactosamine sequences; gangliosides, oligosaccharide fragments of glycosaminoglycans and polysialic acid. The arrays also include designer oligosaccharide probes of microbial and plant-derived homo-oligomers of glucose and of other monosaccharides (Fig. S5). Essential to the analysis system has been the development of a database for storage and scrutiny of microarray data.47 This holds all of the microarray data, the experimental conditions, and information on saccharide probes and proteins. There is an associated interactive software for presentation of microarray data, displaying data as tables, charts, or “matrices,” selective data retrieval, filtering, sorting, and deep mining of every data point.
Affiliation: The Glycosciences Laboratory, Department of Medicine, Imperial College London, London, United Kingdom. email@example.com