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Development of a Nasonia vitripennis outbred laboratory population for genetic analysis.

van de Zande L, Ferber S, de Haan A, Beukeboom LW, van Heerwaarden J, Pannebakker BA - Mol Ecol Resour (2013)

Bottom Line: As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (N(e)) by microsatellite analysis.A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417,331 high-quality SNPs spanning all five Nasonia chromosomes.The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system.

View Article: PubMed Central - PubMed

Affiliation: Evolutionary Genetics, Centre for Ecological and Evolutionary Studies, University of Groningen, 9700 CC, Groningen, the Netherlands.

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Effective population size Ne in the Nasonia vitripennis HVRx outbred laboratory population. Posterior density of the empirical estimate of Ne based on 40 microsatellite loci, obtained by approximate Bayesian computation. Blue, red and green lines indicate the theoretical prediction based on Wright (1933), the posterior mean and posterior median of the empirical estimate, respectively.
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fig02: Effective population size Ne in the Nasonia vitripennis HVRx outbred laboratory population. Posterior density of the empirical estimate of Ne based on 40 microsatellite loci, obtained by approximate Bayesian computation. Blue, red and green lines indicate the theoretical prediction based on Wright (1933), the posterior mean and posterior median of the empirical estimate, respectively.

Mentions: The empirical estimation of effective population size (Ne), based on the diversity parameters above, yielded a posterior mode of 195 and a mean of 236 diploid individuals. The computer simulations of the different breeding schemes yielded nearly identical effective population sizes of Ne = 177 and Ne = 173 for the four-replicate and single-replicate mating scheme, respectively, entirely consistent with the theoretical prediction of Ne = 173 and falling within 0.9 and 0.3 standard deviations of the posterior mean and mode, respectively (Fig. 2).


Development of a Nasonia vitripennis outbred laboratory population for genetic analysis.

van de Zande L, Ferber S, de Haan A, Beukeboom LW, van Heerwaarden J, Pannebakker BA - Mol Ecol Resour (2013)

Effective population size Ne in the Nasonia vitripennis HVRx outbred laboratory population. Posterior density of the empirical estimate of Ne based on 40 microsatellite loci, obtained by approximate Bayesian computation. Blue, red and green lines indicate the theoretical prediction based on Wright (1933), the posterior mean and posterior median of the empirical estimate, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4260118&req=5

fig02: Effective population size Ne in the Nasonia vitripennis HVRx outbred laboratory population. Posterior density of the empirical estimate of Ne based on 40 microsatellite loci, obtained by approximate Bayesian computation. Blue, red and green lines indicate the theoretical prediction based on Wright (1933), the posterior mean and posterior median of the empirical estimate, respectively.
Mentions: The empirical estimation of effective population size (Ne), based on the diversity parameters above, yielded a posterior mode of 195 and a mean of 236 diploid individuals. The computer simulations of the different breeding schemes yielded nearly identical effective population sizes of Ne = 177 and Ne = 173 for the four-replicate and single-replicate mating scheme, respectively, entirely consistent with the theoretical prediction of Ne = 173 and falling within 0.9 and 0.3 standard deviations of the posterior mean and mode, respectively (Fig. 2).

Bottom Line: As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (N(e)) by microsatellite analysis.A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417,331 high-quality SNPs spanning all five Nasonia chromosomes.The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system.

View Article: PubMed Central - PubMed

Affiliation: Evolutionary Genetics, Centre for Ecological and Evolutionary Studies, University of Groningen, 9700 CC, Groningen, the Netherlands.

Show MeSH
Related in: MedlinePlus