Development of a Nasonia vitripennis outbred laboratory population for genetic analysis.
Bottom Line: The release of its genome sequence led to the development of high-resolution genomic tools, for both interspecific and intraspecific research, which has resulted in great advances in understanding Nasonia biology.As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (N(e)) by microsatellite analysis.The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system.
Affiliation: Evolutionary Genetics, Centre for Ecological and Evolutionary Studies, University of Groningen, 9700 CC, Groningen, the Netherlands.Show MeSH
Related in: MedlinePlus
Mentions: The empirical estimation of effective population size (Ne), based on the diversity parameters above, yielded a posterior mode of 195 and a mean of 236 diploid individuals. The computer simulations of the different breeding schemes yielded nearly identical effective population sizes of Ne = 177 and Ne = 173 for the four-replicate and single-replicate mating scheme, respectively, entirely consistent with the theoretical prediction of Ne = 173 and falling within 0.9 and 0.3 standard deviations of the posterior mean and mode, respectively (Fig. 2).
Affiliation: Evolutionary Genetics, Centre for Ecological and Evolutionary Studies, University of Groningen, 9700 CC, Groningen, the Netherlands.