Development of a Nasonia vitripennis outbred laboratory population for genetic analysis.
Bottom Line: As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (N(e)) by microsatellite analysis.A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417,331 high-quality SNPs spanning all five Nasonia chromosomes.The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system.
Affiliation: Evolutionary Genetics, Centre for Ecological and Evolutionary Studies, University of Groningen, 9700 CC, Groningen, the Netherlands.Show MeSH
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Mentions: The empirical estimation of effective population size (Ne), based on the diversity parameters above, yielded a posterior mode of 195 and a mean of 236 diploid individuals. The computer simulations of the different breeding schemes yielded nearly identical effective population sizes of Ne = 177 and Ne = 173 for the four-replicate and single-replicate mating scheme, respectively, entirely consistent with the theoretical prediction of Ne = 173 and falling within 0.9 and 0.3 standard deviations of the posterior mean and mode, respectively (Fig. 2).
Affiliation: Evolutionary Genetics, Centre for Ecological and Evolutionary Studies, University of Groningen, 9700 CC, Groningen, the Netherlands.