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Development of a Nasonia vitripennis outbred laboratory population for genetic analysis.

van de Zande L, Ferber S, de Haan A, Beukeboom LW, van Heerwaarden J, Pannebakker BA - Mol Ecol Resour (2013)

Bottom Line: As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (N(e)) by microsatellite analysis.A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417,331 high-quality SNPs spanning all five Nasonia chromosomes.The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system.

View Article: PubMed Central - PubMed

Affiliation: Evolutionary Genetics, Centre for Ecological and Evolutionary Studies, University of Groningen, 9700 CC, Groningen, the Netherlands.

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Nasonia vitripennis  HVRx outbred laboratory population maintenance schedule. In each of four mass culture tubes, 40 mated female N. vitripennis wasps of generation x are provided with 50 Calliphora spp. hosts. After oviposition, the parasitized hosts are redistributed over four clean mass culture tubes, to ensure optimal mixing of the wasps over all four culture tubes and to allow mating between all wasps emerging within generation x + 1.
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fig01: Nasonia vitripennis HVRx outbred laboratory population maintenance schedule. In each of four mass culture tubes, 40 mated female N. vitripennis wasps of generation x are provided with 50 Calliphora spp. hosts. After oviposition, the parasitized hosts are redistributed over four clean mass culture tubes, to ensure optimal mixing of the wasps over all four culture tubes and to allow mating between all wasps emerging within generation x + 1.

Mentions: To maximize genetic diversity, we merged the HV1 and HV2 replicates to form one single HVRx population after approximately 36 generations of separate mass culturing. From both replicates, we hosted 80 mated females on 200 hosts, and the offspring was allowed to mate randomly before dividing the wasps over four mass culture tubes (70 × 20 mm) each containing 50 fly hosts for oviposition. After 1 week, the parasitized hosts were distributed over four new mass culture tubes. After emergence, approximately 30 mated females from each mass culture tube were transferred to new mass culture tubes to initiate the next generation (Fig. 1). This breeding procedure ensures the maintenance of a large outbred population by allowing random mating between wasps of different tubes and was designed to preserve genetic diversity across generations. The HVRx population was maintained on C. vicina pupae as hosts, at 25 °C, 16: 8 light:dark conditions.


Development of a Nasonia vitripennis outbred laboratory population for genetic analysis.

van de Zande L, Ferber S, de Haan A, Beukeboom LW, van Heerwaarden J, Pannebakker BA - Mol Ecol Resour (2013)

Nasonia vitripennis  HVRx outbred laboratory population maintenance schedule. In each of four mass culture tubes, 40 mated female N. vitripennis wasps of generation x are provided with 50 Calliphora spp. hosts. After oviposition, the parasitized hosts are redistributed over four clean mass culture tubes, to ensure optimal mixing of the wasps over all four culture tubes and to allow mating between all wasps emerging within generation x + 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4260118&req=5

fig01: Nasonia vitripennis HVRx outbred laboratory population maintenance schedule. In each of four mass culture tubes, 40 mated female N. vitripennis wasps of generation x are provided with 50 Calliphora spp. hosts. After oviposition, the parasitized hosts are redistributed over four clean mass culture tubes, to ensure optimal mixing of the wasps over all four culture tubes and to allow mating between all wasps emerging within generation x + 1.
Mentions: To maximize genetic diversity, we merged the HV1 and HV2 replicates to form one single HVRx population after approximately 36 generations of separate mass culturing. From both replicates, we hosted 80 mated females on 200 hosts, and the offspring was allowed to mate randomly before dividing the wasps over four mass culture tubes (70 × 20 mm) each containing 50 fly hosts for oviposition. After 1 week, the parasitized hosts were distributed over four new mass culture tubes. After emergence, approximately 30 mated females from each mass culture tube were transferred to new mass culture tubes to initiate the next generation (Fig. 1). This breeding procedure ensures the maintenance of a large outbred population by allowing random mating between wasps of different tubes and was designed to preserve genetic diversity across generations. The HVRx population was maintained on C. vicina pupae as hosts, at 25 °C, 16: 8 light:dark conditions.

Bottom Line: As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (N(e)) by microsatellite analysis.A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417,331 high-quality SNPs spanning all five Nasonia chromosomes.The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system.

View Article: PubMed Central - PubMed

Affiliation: Evolutionary Genetics, Centre for Ecological and Evolutionary Studies, University of Groningen, 9700 CC, Groningen, the Netherlands.

Show MeSH