Limits...
Hepacivirus infection in domestic horses, Brazil, 2011-2013.

Gemaque BS, Junior Souza de Souza A, do Carmo Pereira Soares M, Malheiros AP, Silva AL, Alves MM, Gomes-Gouvêa MS, Pinho JR, Ferreira de Figueiredo H, Ribeiro DB, Souza da Silva J, Moraes LA, Ribeiro AS, Pereira WL - Emerging Infect. Dis. (2014)

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

: An estimated ≈150 million persons (3% of the world population) are chronically infected with hepatitis C virus (HCV)... Development of an HCV vaccine has been hampered by the difficulty of in vitro cultivation of the agent and by lack of animal models for studies of viral functions and host immune reactions... The only effective experimental model that can be infected with HCV and in which the course of infection is similar to that in humans is the chimpanzee... In this article, we refer to the viruses detected in horses as conventional nonprimate hepaciviruses (NPHVs)... Viral RNA was extracted from serum samples by using the TRIzol LS Reagent (Invitrogen, Carlsbad, CA, USA), and a target sequence in the nonstructural 3 protein (NS3) region of the NPHV genome was amplified from the cDNA by using a previously reported nested PCR protocol... Second-round product reactions of ≈380 bp were considered positive... To phylogenetically analyze the virus sequences based on an NS3 partial nucleotide sequence of 294 bp, we used the maximum-likelihood method with the Kimura 2-parameter model in MEGA version 5.1 (http://www.megasoftware.net) (bootstrap, 1,000 replicates)... KJ469442–KJ469466... Of the 300 serum samples, 25 (8.3%) were positive for NPHV by nested reverse transcription PCR; these results were confirmed by nucleotide sequencing... Until now, it has been impossible to associate Hepacivirus infection in horses with clinical hepatic disease... The nucleotide distances of the sequences ranged from 0 to 19.9%... Many of these new isolates were grouped according to geographic origin, except for 2 sequences that grouped distantly from other isolates from Neotropical regions... The infection seems to be enzootic in the studied horse population and to be geographically dispersed in northeastern Pará State... Locations in the State of Pará (eastern Brazilian Amazon), Brazil, where blood samples were collected from 300 equids for nonprimate hepacivirus testing.

Show MeSH

Related in: MedlinePlus

Maximum-likelihood phylogenetic tree of the partial nucleotide sequences of the nonstructural protein 3 region (294 bp) of Hepacivirus. The retrieved sequences from GenBank are indicated by the accession number followed by the species from which each was isolated. The 25 sequences obtained from 300 equids in 7 cities and 1 district of the State of Pará, Brazil, during January 2011–November 2013, are indicated with a dot and are identified by their GenBank accession numbers followed by their community of origin. Bootstrap values ​​(1,000 replicates) >70% are listed at the nodes. One sequence of equine Pegivirus was used as an outgroup. GBV-B, GB virus B; HCV, hepatitis C virus; NPHV, nonprimate hepaciviruses. Scale bar indicates nucleotide substitutions per site.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4257787&req=5

Figure 1: Maximum-likelihood phylogenetic tree of the partial nucleotide sequences of the nonstructural protein 3 region (294 bp) of Hepacivirus. The retrieved sequences from GenBank are indicated by the accession number followed by the species from which each was isolated. The 25 sequences obtained from 300 equids in 7 cities and 1 district of the State of Pará, Brazil, during January 2011–November 2013, are indicated with a dot and are identified by their GenBank accession numbers followed by their community of origin. Bootstrap values ​​(1,000 replicates) >70% are listed at the nodes. One sequence of equine Pegivirus was used as an outgroup. GBV-B, GB virus B; HCV, hepatitis C virus; NPHV, nonprimate hepaciviruses. Scale bar indicates nucleotide substitutions per site.

Mentions: The sequences reported in this study shared close genetic relationships with previously reported NPHV sequences from dogs and horses (Figure) (2,6–8). The nucleotide distances of the sequences ranged from 0 to 19.9%.


Hepacivirus infection in domestic horses, Brazil, 2011-2013.

Gemaque BS, Junior Souza de Souza A, do Carmo Pereira Soares M, Malheiros AP, Silva AL, Alves MM, Gomes-Gouvêa MS, Pinho JR, Ferreira de Figueiredo H, Ribeiro DB, Souza da Silva J, Moraes LA, Ribeiro AS, Pereira WL - Emerging Infect. Dis. (2014)

Maximum-likelihood phylogenetic tree of the partial nucleotide sequences of the nonstructural protein 3 region (294 bp) of Hepacivirus. The retrieved sequences from GenBank are indicated by the accession number followed by the species from which each was isolated. The 25 sequences obtained from 300 equids in 7 cities and 1 district of the State of Pará, Brazil, during January 2011–November 2013, are indicated with a dot and are identified by their GenBank accession numbers followed by their community of origin. Bootstrap values ​​(1,000 replicates) >70% are listed at the nodes. One sequence of equine Pegivirus was used as an outgroup. GBV-B, GB virus B; HCV, hepatitis C virus; NPHV, nonprimate hepaciviruses. Scale bar indicates nucleotide substitutions per site.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4257787&req=5

Figure 1: Maximum-likelihood phylogenetic tree of the partial nucleotide sequences of the nonstructural protein 3 region (294 bp) of Hepacivirus. The retrieved sequences from GenBank are indicated by the accession number followed by the species from which each was isolated. The 25 sequences obtained from 300 equids in 7 cities and 1 district of the State of Pará, Brazil, during January 2011–November 2013, are indicated with a dot and are identified by their GenBank accession numbers followed by their community of origin. Bootstrap values ​​(1,000 replicates) >70% are listed at the nodes. One sequence of equine Pegivirus was used as an outgroup. GBV-B, GB virus B; HCV, hepatitis C virus; NPHV, nonprimate hepaciviruses. Scale bar indicates nucleotide substitutions per site.
Mentions: The sequences reported in this study shared close genetic relationships with previously reported NPHV sequences from dogs and horses (Figure) (2,6–8). The nucleotide distances of the sequences ranged from 0 to 19.9%.

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

: An estimated ≈150 million persons (3% of the world population) are chronically infected with hepatitis C virus (HCV)... Development of an HCV vaccine has been hampered by the difficulty of in vitro cultivation of the agent and by lack of animal models for studies of viral functions and host immune reactions... The only effective experimental model that can be infected with HCV and in which the course of infection is similar to that in humans is the chimpanzee... In this article, we refer to the viruses detected in horses as conventional nonprimate hepaciviruses (NPHVs)... Viral RNA was extracted from serum samples by using the TRIzol LS Reagent (Invitrogen, Carlsbad, CA, USA), and a target sequence in the nonstructural 3 protein (NS3) region of the NPHV genome was amplified from the cDNA by using a previously reported nested PCR protocol... Second-round product reactions of ≈380 bp were considered positive... To phylogenetically analyze the virus sequences based on an NS3 partial nucleotide sequence of 294 bp, we used the maximum-likelihood method with the Kimura 2-parameter model in MEGA version 5.1 (http://www.megasoftware.net) (bootstrap, 1,000 replicates)... KJ469442–KJ469466... Of the 300 serum samples, 25 (8.3%) were positive for NPHV by nested reverse transcription PCR; these results were confirmed by nucleotide sequencing... Until now, it has been impossible to associate Hepacivirus infection in horses with clinical hepatic disease... The nucleotide distances of the sequences ranged from 0 to 19.9%... Many of these new isolates were grouped according to geographic origin, except for 2 sequences that grouped distantly from other isolates from Neotropical regions... The infection seems to be enzootic in the studied horse population and to be geographically dispersed in northeastern Pará State... Locations in the State of Pará (eastern Brazilian Amazon), Brazil, where blood samples were collected from 300 equids for nonprimate hepacivirus testing.

Show MeSH
Related in: MedlinePlus