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VEGF-C improves regeneration and lymphatic reconnection of transplanted autologous lymph node fragments: An animal model for secondary lymphedema treatment.

Schindewolffs L, Breves G, Buettner M, Hadamitzky C, Pabst R - Immun Inflamm Dis (2014)

Bottom Line: The higher dosage enhanced the reconnection rates significantly and showed a statistical tendency of improving regeneration.An application on early postoperative days and the injection into the medial thigh improved the reconnection significantly.However, these variables did not affect the regeneration statistically.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunomorphology, Hannover Medical School Hannover, Niedersachsen, Germany.

ABSTRACT
Secondary lymphedema occurs after for example breast cancer surgery and radiation in 20-50% of the patients. Due to the poor outcomes of surgical treatments in the past, the therapy often remains symptomatic. However, avascular transplantation of autologous lymph node fragments (LN-Tx) combined with postoperative injections of vascular endothelial growth factor-C (VEGF-C) emerges as a potential surgical therapy. In this study, adult rats underwent LN-Tx to investigate the following parameters of VEGF-C application: time point, location and dosage. Furthermore, the influences of VEGF-C on lymphatic reconnection and transplant regeneration were analyzed. The reconnection was investigated using intradermally injected blue dye and the regeneration was evaluated histologically using hematoxylin-eosin (H&E) staining and immunohistochemistry. The higher dosage enhanced the reconnection rates significantly and showed a statistical tendency of improving regeneration. An application on early postoperative days and the injection into the medial thigh improved the reconnection significantly. However, these variables did not affect the regeneration statistically. This study confirms that LN-Tx combined with lymphatic growth factor VEGF-C is a possible approach in the therapy of secondary lymphedema and shows the important role of VEGF-C application parameters.

No MeSH data available.


Related in: MedlinePlus

Experimental protocol. In every group one VEGF-C application parameter, such as time point, location or dosage was modified. Group A was left without further intervention after surgery (control group) and group B was treated with 240 µL saline solution (saline control group). VEGF-C, vascular endothelial growth factor-C.
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fig01: Experimental protocol. In every group one VEGF-C application parameter, such as time point, location or dosage was modified. Group A was left without further intervention after surgery (control group) and group B was treated with 240 µL saline solution (saline control group). VEGF-C, vascular endothelial growth factor-C.

Mentions: The experimental procedure had been approved by the local authorities (no. 33.9-42502-04-07/1420). For this study, 109 adult female Lewis rats (LEW/Crl, approximately 200 g) were used. The rats were divided into six groups (A–F). Groups A, B, E, and F included 20 rats, group C 10 rats, and group D 19 rats (Fig. 1).


VEGF-C improves regeneration and lymphatic reconnection of transplanted autologous lymph node fragments: An animal model for secondary lymphedema treatment.

Schindewolffs L, Breves G, Buettner M, Hadamitzky C, Pabst R - Immun Inflamm Dis (2014)

Experimental protocol. In every group one VEGF-C application parameter, such as time point, location or dosage was modified. Group A was left without further intervention after surgery (control group) and group B was treated with 240 µL saline solution (saline control group). VEGF-C, vascular endothelial growth factor-C.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4257760&req=5

fig01: Experimental protocol. In every group one VEGF-C application parameter, such as time point, location or dosage was modified. Group A was left without further intervention after surgery (control group) and group B was treated with 240 µL saline solution (saline control group). VEGF-C, vascular endothelial growth factor-C.
Mentions: The experimental procedure had been approved by the local authorities (no. 33.9-42502-04-07/1420). For this study, 109 adult female Lewis rats (LEW/Crl, approximately 200 g) were used. The rats were divided into six groups (A–F). Groups A, B, E, and F included 20 rats, group C 10 rats, and group D 19 rats (Fig. 1).

Bottom Line: The higher dosage enhanced the reconnection rates significantly and showed a statistical tendency of improving regeneration.An application on early postoperative days and the injection into the medial thigh improved the reconnection significantly.However, these variables did not affect the regeneration statistically.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunomorphology, Hannover Medical School Hannover, Niedersachsen, Germany.

ABSTRACT
Secondary lymphedema occurs after for example breast cancer surgery and radiation in 20-50% of the patients. Due to the poor outcomes of surgical treatments in the past, the therapy often remains symptomatic. However, avascular transplantation of autologous lymph node fragments (LN-Tx) combined with postoperative injections of vascular endothelial growth factor-C (VEGF-C) emerges as a potential surgical therapy. In this study, adult rats underwent LN-Tx to investigate the following parameters of VEGF-C application: time point, location and dosage. Furthermore, the influences of VEGF-C on lymphatic reconnection and transplant regeneration were analyzed. The reconnection was investigated using intradermally injected blue dye and the regeneration was evaluated histologically using hematoxylin-eosin (H&E) staining and immunohistochemistry. The higher dosage enhanced the reconnection rates significantly and showed a statistical tendency of improving regeneration. An application on early postoperative days and the injection into the medial thigh improved the reconnection significantly. However, these variables did not affect the regeneration statistically. This study confirms that LN-Tx combined with lymphatic growth factor VEGF-C is a possible approach in the therapy of secondary lymphedema and shows the important role of VEGF-C application parameters.

No MeSH data available.


Related in: MedlinePlus