Limits...
Further characterization of Tsol-p27 as a diagnostic antigen in sub-Saharan Africa.

Nhancupe N, Salazar-Anton F, Noormahomed EV, Afonso S, Lindh J - Exp. Parasitol. (2013)

Bottom Line: Immunoblotting demonstrated that Tsol-p27 was recognized by all 10 serum samples from NCC-positive individuals, whereas cC1 was identified by only five of the 10 positive sera.None of the antigens were recognized by negative control sera.Despite the limited number of serum samples evaluated in this study, the results suggest that Tsol-p27 can be a suitable candidate for diagnosis of human NCC, not only in Central America but also in sub-Saharan Africa.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiologia, Faculdade de Medicina, Universidade Eduardo Mondlane, Mozambique; Microbiology, Tumor and Cell Biology, Karolinska Institutet, Sweden. Electronic address: noemianha@yahoo.com.br.

Show MeSH

Related in: MedlinePlus

Identification of immunoreactive proteins from T. solium cysticerci by 2-DE Western blot analysis. (A) A 2-DE gel stained with Coomassie blue, showing soluble cysticerci proteins with pI values in the range 4–7. (B) Corresponding Western blot probed with serum from a patient diagnosed with NCC. (C) Corresponding Western blot probed with serum from a control subject. Arrows indicate the proteins that were isolated and sequenced (Table 1).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4257600&req=5

Figure 1: Identification of immunoreactive proteins from T. solium cysticerci by 2-DE Western blot analysis. (A) A 2-DE gel stained with Coomassie blue, showing soluble cysticerci proteins with pI values in the range 4–7. (B) Corresponding Western blot probed with serum from a patient diagnosed with NCC. (C) Corresponding Western blot probed with serum from a control subject. Arrows indicate the proteins that were isolated and sequenced (Table 1).

Mentions: The soluble antigens were separated according to their pI. Initially, we used a broad pI range of 4–10, and, based on those experiments, it could be established that the vast majority of soluble antigens had a pI of less than 7, and therefore the remaining experiments were performed at pI 4–7. Proteins separated according to their pI were followed by molecular weight separation and visualized by Coomassie blue staining (Fig. 1a). The separated proteins were transferred to nitrocellulose membranes and probed four times with NCC-positive and NCC-negative sera, respectively. The proteins that were recognized by sera from patients with NCC (Fig. 1b) but not by sera from the NCC-negative subjects (Fig. 1c) were isolated, which enabled us to localize antigenic spots potentially specific for NCC (Fig. 1a).


Further characterization of Tsol-p27 as a diagnostic antigen in sub-Saharan Africa.

Nhancupe N, Salazar-Anton F, Noormahomed EV, Afonso S, Lindh J - Exp. Parasitol. (2013)

Identification of immunoreactive proteins from T. solium cysticerci by 2-DE Western blot analysis. (A) A 2-DE gel stained with Coomassie blue, showing soluble cysticerci proteins with pI values in the range 4–7. (B) Corresponding Western blot probed with serum from a patient diagnosed with NCC. (C) Corresponding Western blot probed with serum from a control subject. Arrows indicate the proteins that were isolated and sequenced (Table 1).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4257600&req=5

Figure 1: Identification of immunoreactive proteins from T. solium cysticerci by 2-DE Western blot analysis. (A) A 2-DE gel stained with Coomassie blue, showing soluble cysticerci proteins with pI values in the range 4–7. (B) Corresponding Western blot probed with serum from a patient diagnosed with NCC. (C) Corresponding Western blot probed with serum from a control subject. Arrows indicate the proteins that were isolated and sequenced (Table 1).
Mentions: The soluble antigens were separated according to their pI. Initially, we used a broad pI range of 4–10, and, based on those experiments, it could be established that the vast majority of soluble antigens had a pI of less than 7, and therefore the remaining experiments were performed at pI 4–7. Proteins separated according to their pI were followed by molecular weight separation and visualized by Coomassie blue staining (Fig. 1a). The separated proteins were transferred to nitrocellulose membranes and probed four times with NCC-positive and NCC-negative sera, respectively. The proteins that were recognized by sera from patients with NCC (Fig. 1b) but not by sera from the NCC-negative subjects (Fig. 1c) were isolated, which enabled us to localize antigenic spots potentially specific for NCC (Fig. 1a).

Bottom Line: Immunoblotting demonstrated that Tsol-p27 was recognized by all 10 serum samples from NCC-positive individuals, whereas cC1 was identified by only five of the 10 positive sera.None of the antigens were recognized by negative control sera.Despite the limited number of serum samples evaluated in this study, the results suggest that Tsol-p27 can be a suitable candidate for diagnosis of human NCC, not only in Central America but also in sub-Saharan Africa.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiologia, Faculdade de Medicina, Universidade Eduardo Mondlane, Mozambique; Microbiology, Tumor and Cell Biology, Karolinska Institutet, Sweden. Electronic address: noemianha@yahoo.com.br.

Show MeSH
Related in: MedlinePlus