Further characterization of Tsol-p27 as a diagnostic antigen in sub-Saharan Africa.
Bottom Line: Immunoblotting demonstrated that Tsol-p27 was recognized by all 10 serum samples from NCC-positive individuals, whereas cC1 was identified by only five of the 10 positive sera.None of the antigens were recognized by negative control sera.Despite the limited number of serum samples evaluated in this study, the results suggest that Tsol-p27 can be a suitable candidate for diagnosis of human NCC, not only in Central America but also in sub-Saharan Africa.
Affiliation: Departamento de Microbiologia, Faculdade de Medicina, Universidade Eduardo Mondlane, Mozambique; Microbiology, Tumor and Cell Biology, Karolinska Institutet, Sweden. Electronic address: email@example.com.Show MeSH
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Mentions: The soluble antigens were separated according to their pI. Initially, we used a broad pI range of 4–10, and, based on those experiments, it could be established that the vast majority of soluble antigens had a pI of less than 7, and therefore the remaining experiments were performed at pI 4–7. Proteins separated according to their pI were followed by molecular weight separation and visualized by Coomassie blue staining (Fig. 1a). The separated proteins were transferred to nitrocellulose membranes and probed four times with NCC-positive and NCC-negative sera, respectively. The proteins that were recognized by sera from patients with NCC (Fig. 1b) but not by sera from the NCC-negative subjects (Fig. 1c) were isolated, which enabled us to localize antigenic spots potentially specific for NCC (Fig. 1a).
Affiliation: Departamento de Microbiologia, Faculdade de Medicina, Universidade Eduardo Mondlane, Mozambique; Microbiology, Tumor and Cell Biology, Karolinska Institutet, Sweden. Electronic address: firstname.lastname@example.org.