Detecting cryptic indirect genetic effects.
Bottom Line: Using a multiple-regression approach, we identified male startle response as a candidate interacting phenotype: the longer it took interacting males to settle after being startled, the less focal males tapped them.A genome-wide association analysis identified approximately a dozen candidate protein-coding genes potentially underlying the IGE, of which the most significant was slowpoke.Our methodological framework provides information about candidate phenotypes and candidate single-nucleotide polymorphisms that underpin a strong yet cryptic IGE.
Affiliation: Centre for Biological Diversity, University of St Andrews, St Andrews, Fife KY16 9TH, United Kingdom. firstname.lastname@example.org.Show MeSH
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Mentions: The GWA identified 13 SNPs matching our significance criterion (Fig.6). These were located on all chromosomes, and included SNPs in intronic regions near eight annotated genes. Mean coverage ranged from 6 × to 25 ×, and effect sizes ranged from −0.114 to 0.076. The most significant SNP was located in a 3′ untranscribed region of the protein-coding gene slowpoke (slo). Other SNPs were located in introns of, or sequences near, the transcription factor Myocyte enhancer factor 2 (Mef2), and the protein-coding genes Proteasome α6 subunit (Pros α6), happyhour (hppy), Br140, Neuropilin and tolloid-like (Neto), Histidyl-tRNA synthetase (Aats-his), and Cadherin 87A (Cad87A).
Affiliation: Centre for Biological Diversity, University of St Andrews, St Andrews, Fife KY16 9TH, United Kingdom. email@example.com.