Exposure to inflammatory cytokines selectively limits GM-CSF production by induced T regulatory cells.
Bottom Line: Understanding processes that can limit this potentially deleterious effect of Treg cells in a therapeutic setting is therefore important.We show that iTreg cells can produce significant amounts of three proinflammatory cytokines (IFN-γ, GM-CSF and TNF-α) upon secondary TCR stimulation.Furthermore, we show that IL-6 and IL-27 individually, or IL-2 and TGF-β in combination, can mediate the selective loss of GM-CSF production by iTreg cells.
Affiliation: MRC Centre for Inflammation Research, University of Edinburgh, Edinburgh, UK.Show MeSH
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Mentions: The results in Fig. 4H–J suggested that component(s) of the in vivo inflammatory milieu were capable of selectively degrading the ability of iTreg cells to produce GM-CSF while maintaining IFN-γ and TNF-α production. To understand whether inflammatory cytokine(s) might be responsible for this, we returned to the in vitro restimulation of iTreg cells either under “neutral” conditions, or in the presence of additional cytokines (Fig. 5).
Affiliation: MRC Centre for Inflammation Research, University of Edinburgh, Edinburgh, UK.