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Urethral reconstruction with tissue-engineered human amniotic scaffold in rabbit urethral injury models.

Wang F, Liu T, Yang L, Zhang G, Liu H, Yi X, Yang X, Lin TY, Qin W, Yuan J - Med. Sci. Monit. (2014)

Bottom Line: After the successful acquisition of dHAS from AM, cell-seeded dHAS were prepared and characterized.Immune responses were compared by histological evaluation and CD4 cell and CD8 cell infiltrations.Histopathological analysis revealed mild immune response in cell-seeded dHAS group (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China (mainland).

ABSTRACT

Background: Mitigating urethral injury remains a great challenge for urologists due to lack of ideal biomaterials for urethroplasty. The application of amniotic membranes (AM) over other synthetic materials make it a better potential source for urethral reconstruction. We separated the basement layer of AM to obtain denuded human amniotic scaffold (dHAS) and then inoculated primary rabbit urethral epithelial cells on the surface of dHAS to define whether this strategy minimize potential rejection and maximize the biocompatibility of human AM.

Material/methods: After the successful acquisition of dHAS from AM, cell-seeded dHAS were prepared and characterized. Both cell-seeded dHAS and acellular dHAS were subcutaneously implanted. Immune responses were compared by histological evaluation and CD4 cell and CD8 cell infiltrations. Then they were applied as urethroplastic materials in the rabbit models of urethral injury to fully explore the feasibility and efficacy of tissue-engineered dHAS xenografts in urethral substitution application.

Results: Mild inflammatory infiltration was observed in cell-seeded dHAS grafts, as revealed by fewer accumulations of CD4 cells and CD8 cells (or neutrophils or other immune cells). Urethral defects of rabbits in the urethroplastic group with dHAS implantation (n=6) were completely resolved in one month, while there were one infection and one fistula in the control group with acellular dHAS patches (n=6). Histopathological analysis revealed mild immune response in cell-seeded dHAS group (P<0.05).

Conclusions: Tissue-engineered dHAS minimize potential rejection and maximize the biocompatibility of AM, which makes it a potential ideal xenograft for urethral reconstruction.

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Related in: MedlinePlus

HE staining of the cell-seeded dHAS and dHAS after subcutaneous implantation. (A–D): HE stainings of cell-seeded dHAS engrafts at week 1, 2, 4, and 8 after subcutaneous implantation; (E–H): HE stainings of dHAS engrafts at week 1, 2, 4, and 8. Fewer inflammatory infiltrations in dHAS xenografts were found, in comparison to AM grafts (magnification at ×200).
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f3-medscimonit-20-2430: HE staining of the cell-seeded dHAS and dHAS after subcutaneous implantation. (A–D): HE stainings of cell-seeded dHAS engrafts at week 1, 2, 4, and 8 after subcutaneous implantation; (E–H): HE stainings of dHAS engrafts at week 1, 2, 4, and 8. Fewer inflammatory infiltrations in dHAS xenografts were found, in comparison to AM grafts (magnification at ×200).

Mentions: To investigate the histocompatibility of cell-seeded dHAS, the prepared dHAS and cell-seeded dHAS were implanted subcutaneously to posterior portions of rabbits. In the follow-up studies with duration up to 8 weeks, no serious inflammation or rejection was observed in the cell-seeded dHAS implantation group, as indicated by HE staining (Figure 3), as well as the scant infiltration of CD4+ and CD8+ cells revealed through immunohistochemical analysis (Figure 4). Apparent accumulation of CD4+ and CD8+ cells was found in the group that underwent AM implantation. There was a significant statistical difference (p<0.05) in CD4+ and CD8+ infiltrations between these 2 groups (Figure 4E).


Urethral reconstruction with tissue-engineered human amniotic scaffold in rabbit urethral injury models.

Wang F, Liu T, Yang L, Zhang G, Liu H, Yi X, Yang X, Lin TY, Qin W, Yuan J - Med. Sci. Monit. (2014)

HE staining of the cell-seeded dHAS and dHAS after subcutaneous implantation. (A–D): HE stainings of cell-seeded dHAS engrafts at week 1, 2, 4, and 8 after subcutaneous implantation; (E–H): HE stainings of dHAS engrafts at week 1, 2, 4, and 8. Fewer inflammatory infiltrations in dHAS xenografts were found, in comparison to AM grafts (magnification at ×200).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4257484&req=5

f3-medscimonit-20-2430: HE staining of the cell-seeded dHAS and dHAS after subcutaneous implantation. (A–D): HE stainings of cell-seeded dHAS engrafts at week 1, 2, 4, and 8 after subcutaneous implantation; (E–H): HE stainings of dHAS engrafts at week 1, 2, 4, and 8. Fewer inflammatory infiltrations in dHAS xenografts were found, in comparison to AM grafts (magnification at ×200).
Mentions: To investigate the histocompatibility of cell-seeded dHAS, the prepared dHAS and cell-seeded dHAS were implanted subcutaneously to posterior portions of rabbits. In the follow-up studies with duration up to 8 weeks, no serious inflammation or rejection was observed in the cell-seeded dHAS implantation group, as indicated by HE staining (Figure 3), as well as the scant infiltration of CD4+ and CD8+ cells revealed through immunohistochemical analysis (Figure 4). Apparent accumulation of CD4+ and CD8+ cells was found in the group that underwent AM implantation. There was a significant statistical difference (p<0.05) in CD4+ and CD8+ infiltrations between these 2 groups (Figure 4E).

Bottom Line: After the successful acquisition of dHAS from AM, cell-seeded dHAS were prepared and characterized.Immune responses were compared by histological evaluation and CD4 cell and CD8 cell infiltrations.Histopathological analysis revealed mild immune response in cell-seeded dHAS group (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China (mainland).

ABSTRACT

Background: Mitigating urethral injury remains a great challenge for urologists due to lack of ideal biomaterials for urethroplasty. The application of amniotic membranes (AM) over other synthetic materials make it a better potential source for urethral reconstruction. We separated the basement layer of AM to obtain denuded human amniotic scaffold (dHAS) and then inoculated primary rabbit urethral epithelial cells on the surface of dHAS to define whether this strategy minimize potential rejection and maximize the biocompatibility of human AM.

Material/methods: After the successful acquisition of dHAS from AM, cell-seeded dHAS were prepared and characterized. Both cell-seeded dHAS and acellular dHAS were subcutaneously implanted. Immune responses were compared by histological evaluation and CD4 cell and CD8 cell infiltrations. Then they were applied as urethroplastic materials in the rabbit models of urethral injury to fully explore the feasibility and efficacy of tissue-engineered dHAS xenografts in urethral substitution application.

Results: Mild inflammatory infiltration was observed in cell-seeded dHAS grafts, as revealed by fewer accumulations of CD4 cells and CD8 cells (or neutrophils or other immune cells). Urethral defects of rabbits in the urethroplastic group with dHAS implantation (n=6) were completely resolved in one month, while there were one infection and one fistula in the control group with acellular dHAS patches (n=6). Histopathological analysis revealed mild immune response in cell-seeded dHAS group (P<0.05).

Conclusions: Tissue-engineered dHAS minimize potential rejection and maximize the biocompatibility of AM, which makes it a potential ideal xenograft for urethral reconstruction.

Show MeSH
Related in: MedlinePlus