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Measurement of free radicals using electron paramagnetic resonance spectroscopy during open aorto-iliac arterial reconstruction.

Majewski W, Krzyminiewski R, Stanisić M, Iskra M, Krasiński Z, Nowak M, Dobosz B - Med. Sci. Monit. (2014)

Bottom Line: HO-1 concentration increased to above the level before intervention 24 h after surgery.Twenty-four hours after surgery, inflammatory markers increased in AAA to CRP was 14.76 ml/l (0.23-38.55), IL-6 was 141.22 pg/ml (84.3-591.03), TNF-alfa was 6.82 pg/ml (1.76-80.01) and AIOD: CRP was 18.44 mg/l (2.56-33.14), IL-6: 184.1 pg/ml (128.46-448.03), TNF-alfa was 7.74 pg/ml (1.74-74.74).Elevated free radical levels decreased 24 h after surgery due to various endogenous antioxidants and therapies.

View Article: PubMed Central - PubMed

Affiliation: Department of General and Vascular Surgery, Poznań University of Medical Sciences, Poznań, Poland.

ABSTRACT

Background: Aortic cross-clamping during abdominal aortic aneurysm (AAA) open repair leads to development of ischemia-reperfusion injury. Electron paramagnetic resonance spectroscopy (EPR) spin-trapping is a valuable method of direct measurement of free radicals. The objective of the study was to evaluate the results of EPR as a direct method of free radical measurement and degree of inflammatory response in open operative treatment of patients with AAA and aorto-iliac occlusive disease (AIOD).

Material/methods: The study was performed on a group of 32 patients with AAA and 25 patients with AIOD scheduled for open repair. Peripheral venous blood for EPR spectroscopy and for SOD, GPx, ox-LDL, Il-6, TNF-alfa, CRP, and HO-1 were harvested. Selected parameters were established accordingly to specified EPR and immunohistochemical methods and analyzed between groups by Mann-Whitney U test and Wilcoxon matched-pairs signed-ranks test with Bonferroni correction.

Results: Free radicals level was correlated with the time of the aortic cross-clamping after the reperfusion of he first and second leg in AAA (r=0.7; r=0.47). ox-LDL in AAA decreased 5 min after reperfusion of the first leg (32.99 U/L, range: 14.09-77.12) and 5 min after reperfusion of the second leg (26.75 U/L, range: 11.56-82.12) and 24 h after the operation (25.85 U/L, range: 14.29-49.70). HO-1 concentration increased to above the level before intervention 24 h after surgery. The activities of GPx and SOD decreased 5 min after the first-leg reperfusion in AAA. Twenty-four hours after surgery, inflammatory markers increased in AAA to CRP was 14.76 ml/l (0.23-38.55), IL-6 was 141.22 pg/ml (84.3-591.03), TNF-alfa was 6.82 pg/ml (1.76-80.01) and AIOD: CRP was 18.44 mg/l (2.56-33.14), IL-6: 184.1 pg/ml (128.46-448.03), TNF-alfa was 7.74 pg/ml (1.74-74.74).

Conclusions: EPR spin-trapping demonstrates temporarily elevated level of free radicals in early phase of reperfusion, leading to decrease antioxidants in AAA. Elevated free radical levels decreased 24 h after surgery due to various endogenous antioxidants and therapies.

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Related in: MedlinePlus

EPR spectra typical for the patient’s blood mixed with nitrosobenzene: (A) recorded at 170K, (B) at room temperature.
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f1-medscimonit-20-2453: EPR spectra typical for the patient’s blood mixed with nitrosobenzene: (A) recorded at 170K, (B) at room temperature.

Mentions: The highest radicals concentration was observed after aortic clamp removal. The measured median values of free radicals by EPR before induction of anesthesia, pre-release of aortic clamp, 5 min after aortic clamp removal, and reperfusion of first leg, 5 min after ipsilateral branch of prosthesis clamp removal, and reperfusion of second leg and 24 h after the operation are summarized in Table 2. Median values of the free radicals revealed a significant elevation after 5 min of the reperfusion of the first leg in surgical treatment of patients with AAA. In both groups of patients, 24 h after surgery, free radicals concentration decreased to a value similar to that before the operation. The values of MDA 5 min after aortic clamp removal and reperfusion of the first leg and also after the reperfusion of the second leg were increased significantly in AAA (Table 3). The changes in free radicals level significantly was correlated with the time of aortic cross-clamping after the reperfusion of the first and second leg in patients with AAA (r=0.7; r=0.47). Typical spectra of AAA samples are presented in Figure 1.


Measurement of free radicals using electron paramagnetic resonance spectroscopy during open aorto-iliac arterial reconstruction.

Majewski W, Krzyminiewski R, Stanisić M, Iskra M, Krasiński Z, Nowak M, Dobosz B - Med. Sci. Monit. (2014)

EPR spectra typical for the patient’s blood mixed with nitrosobenzene: (A) recorded at 170K, (B) at room temperature.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4257482&req=5

f1-medscimonit-20-2453: EPR spectra typical for the patient’s blood mixed with nitrosobenzene: (A) recorded at 170K, (B) at room temperature.
Mentions: The highest radicals concentration was observed after aortic clamp removal. The measured median values of free radicals by EPR before induction of anesthesia, pre-release of aortic clamp, 5 min after aortic clamp removal, and reperfusion of first leg, 5 min after ipsilateral branch of prosthesis clamp removal, and reperfusion of second leg and 24 h after the operation are summarized in Table 2. Median values of the free radicals revealed a significant elevation after 5 min of the reperfusion of the first leg in surgical treatment of patients with AAA. In both groups of patients, 24 h after surgery, free radicals concentration decreased to a value similar to that before the operation. The values of MDA 5 min after aortic clamp removal and reperfusion of the first leg and also after the reperfusion of the second leg were increased significantly in AAA (Table 3). The changes in free radicals level significantly was correlated with the time of aortic cross-clamping after the reperfusion of the first and second leg in patients with AAA (r=0.7; r=0.47). Typical spectra of AAA samples are presented in Figure 1.

Bottom Line: HO-1 concentration increased to above the level before intervention 24 h after surgery.Twenty-four hours after surgery, inflammatory markers increased in AAA to CRP was 14.76 ml/l (0.23-38.55), IL-6 was 141.22 pg/ml (84.3-591.03), TNF-alfa was 6.82 pg/ml (1.76-80.01) and AIOD: CRP was 18.44 mg/l (2.56-33.14), IL-6: 184.1 pg/ml (128.46-448.03), TNF-alfa was 7.74 pg/ml (1.74-74.74).Elevated free radical levels decreased 24 h after surgery due to various endogenous antioxidants and therapies.

View Article: PubMed Central - PubMed

Affiliation: Department of General and Vascular Surgery, Poznań University of Medical Sciences, Poznań, Poland.

ABSTRACT

Background: Aortic cross-clamping during abdominal aortic aneurysm (AAA) open repair leads to development of ischemia-reperfusion injury. Electron paramagnetic resonance spectroscopy (EPR) spin-trapping is a valuable method of direct measurement of free radicals. The objective of the study was to evaluate the results of EPR as a direct method of free radical measurement and degree of inflammatory response in open operative treatment of patients with AAA and aorto-iliac occlusive disease (AIOD).

Material/methods: The study was performed on a group of 32 patients with AAA and 25 patients with AIOD scheduled for open repair. Peripheral venous blood for EPR spectroscopy and for SOD, GPx, ox-LDL, Il-6, TNF-alfa, CRP, and HO-1 were harvested. Selected parameters were established accordingly to specified EPR and immunohistochemical methods and analyzed between groups by Mann-Whitney U test and Wilcoxon matched-pairs signed-ranks test with Bonferroni correction.

Results: Free radicals level was correlated with the time of the aortic cross-clamping after the reperfusion of he first and second leg in AAA (r=0.7; r=0.47). ox-LDL in AAA decreased 5 min after reperfusion of the first leg (32.99 U/L, range: 14.09-77.12) and 5 min after reperfusion of the second leg (26.75 U/L, range: 11.56-82.12) and 24 h after the operation (25.85 U/L, range: 14.29-49.70). HO-1 concentration increased to above the level before intervention 24 h after surgery. The activities of GPx and SOD decreased 5 min after the first-leg reperfusion in AAA. Twenty-four hours after surgery, inflammatory markers increased in AAA to CRP was 14.76 ml/l (0.23-38.55), IL-6 was 141.22 pg/ml (84.3-591.03), TNF-alfa was 6.82 pg/ml (1.76-80.01) and AIOD: CRP was 18.44 mg/l (2.56-33.14), IL-6: 184.1 pg/ml (128.46-448.03), TNF-alfa was 7.74 pg/ml (1.74-74.74).

Conclusions: EPR spin-trapping demonstrates temporarily elevated level of free radicals in early phase of reperfusion, leading to decrease antioxidants in AAA. Elevated free radical levels decreased 24 h after surgery due to various endogenous antioxidants and therapies.

Show MeSH
Related in: MedlinePlus