Limits...
Mycorrhizal-induced calmodulin mediated changes in antioxidant enzymes and growth response of drought-stressed trifoliate orange.

Huang YM, Srivastava AK, Zou YN, Ni QD, Han Y, Wu QS - Front Microbiol (2014)

Bottom Line: A 58-day of DS significantly decreased mycorrhizal colonization by 60% than WW.Compared to non-AM seedlings, AM seedlings displayed significantly higher shoot morphological properties (plant height, stem diameter, and leaf number), biomass production (shoot and root fresh weight) and leaf RWC, regardless of soil water status.The AM seedlings also exhibited significantly higher Cu/Zn-SOD and Mn-SOD activities than the non-AM seedlings under DS but not under WW, which are triggered by higher CaM levels in AM plants on the basis of correlation studies.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture and Gardening/Institute of Root Biology, Yangtze University Jingzhou, China.

ABSTRACT
Trifoliate orange [Poncirus trifoliata (L) Raf.] is considered highly arbuscular mycorrhizal (AM) dependent for growth responses through a series of signal transductions in form of various physiological responses. The proposed study was carried out to evaluate the effect of an AM fungus (Funneliformis mosseae) on growth, antioxidant enzyme (catalase, CAT; superoxide dismutase, SOD) activities, leaf relative water content (RWC), calmodulin (CaM), superoxide anion ([Formula: see text]), and hydrogen peroxide (H2O2) concentrations in leaves of the plants exposed to both well-watered (WW) and drought stress (DS) conditions. A 58-day of DS significantly decreased mycorrhizal colonization by 60% than WW. Compared to non-AM seedlings, AM seedlings displayed significantly higher shoot morphological properties (plant height, stem diameter, and leaf number), biomass production (shoot and root fresh weight) and leaf RWC, regardless of soil water status. AM inoculation significantly increased CaM and soluble protein concentrations and CAT activity, whereas significantly decreased [Formula: see text] and H2O2 concentration under both WW and DS conditions. The AM seedlings also exhibited significantly higher Cu/Zn-SOD and Mn-SOD activities than the non-AM seedlings under DS but not under WW, which are triggered by higher CaM levels in AM plants on the basis of correlation studies. Further, the negative correlation of Cu/Zn-SOD and Mn-SOD activities with [Formula: see text] and H2O2 concentration showed the DS-induced ROS scavenging ability of CaM mediated SODs under mycorrhization. Our results demonstrated that AM-inoculation elevated the synthesis of CaM in leaves and up-regulated activities of the antioxidant enzymes, thereby, repairing the possible oxidative damage to plants by lowering the ROS accumulation under DS condition.

No MeSH data available.


Related in: MedlinePlus

Line regression between CaM concentration and (A) or H2O2(B) concentration in leaves of trifoliate orange inoculated with an AM fungus (F. mosseae) under WW and DS conditions (n = 16).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4257356&req=5

Figure 6: Line regression between CaM concentration and (A) or H2O2(B) concentration in leaves of trifoliate orange inoculated with an AM fungus (F. mosseae) under WW and DS conditions (n = 16).

Mentions: The DS treatment induced significant decrease of leaf CaM concentration than WW treatment, irrespective of AMF- or non-AMF-seedlings status (Figure 3). However, mycorrhizal inoculation significantly increased leaf CaM concentration by 11% under both WW as well as DS conditions (Figure 3). Line regression analysis further supported that leaf CaM concentration was significantly (P < 0.01) positively correlated with mycorrhizal colonization (Figure 4), suggesting that root AM colonization modulated leaf CaM levels, or CaM as the second messenger involved in root mycorrhizal colonization. There were no significant differences of leaf CaM concentration between AMF-seedlings under DS conditions and non-AMF-seedlings under WW conditions. Leaf CaM concentration was significantly positively correlated with leaf SODs (Cu/Zn-SOD and Mn-SOD; Figure 5A) and CAT activity (Figure 5B), but negatively correlated with leaf (Figure 6A) and H2O2 concentration (Figure 6B). Our studies, hence, revealed that AMF inoculation induced CaM mediated elevation in antioxidant enzyme activities and reduction in ROS levels. Interestingly, CaM is reported to induce ROS generation as a second messenger mediating signal transduction under various stress conditions (Bowler and Fluhr, 2000; Chen et al., 2004). It seems that CaM is postulated to be of multiple function protein involved in a series of responses, collectively attributing towards plant defense signal network.


Mycorrhizal-induced calmodulin mediated changes in antioxidant enzymes and growth response of drought-stressed trifoliate orange.

Huang YM, Srivastava AK, Zou YN, Ni QD, Han Y, Wu QS - Front Microbiol (2014)

Line regression between CaM concentration and (A) or H2O2(B) concentration in leaves of trifoliate orange inoculated with an AM fungus (F. mosseae) under WW and DS conditions (n = 16).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4257356&req=5

Figure 6: Line regression between CaM concentration and (A) or H2O2(B) concentration in leaves of trifoliate orange inoculated with an AM fungus (F. mosseae) under WW and DS conditions (n = 16).
Mentions: The DS treatment induced significant decrease of leaf CaM concentration than WW treatment, irrespective of AMF- or non-AMF-seedlings status (Figure 3). However, mycorrhizal inoculation significantly increased leaf CaM concentration by 11% under both WW as well as DS conditions (Figure 3). Line regression analysis further supported that leaf CaM concentration was significantly (P < 0.01) positively correlated with mycorrhizal colonization (Figure 4), suggesting that root AM colonization modulated leaf CaM levels, or CaM as the second messenger involved in root mycorrhizal colonization. There were no significant differences of leaf CaM concentration between AMF-seedlings under DS conditions and non-AMF-seedlings under WW conditions. Leaf CaM concentration was significantly positively correlated with leaf SODs (Cu/Zn-SOD and Mn-SOD; Figure 5A) and CAT activity (Figure 5B), but negatively correlated with leaf (Figure 6A) and H2O2 concentration (Figure 6B). Our studies, hence, revealed that AMF inoculation induced CaM mediated elevation in antioxidant enzyme activities and reduction in ROS levels. Interestingly, CaM is reported to induce ROS generation as a second messenger mediating signal transduction under various stress conditions (Bowler and Fluhr, 2000; Chen et al., 2004). It seems that CaM is postulated to be of multiple function protein involved in a series of responses, collectively attributing towards plant defense signal network.

Bottom Line: A 58-day of DS significantly decreased mycorrhizal colonization by 60% than WW.Compared to non-AM seedlings, AM seedlings displayed significantly higher shoot morphological properties (plant height, stem diameter, and leaf number), biomass production (shoot and root fresh weight) and leaf RWC, regardless of soil water status.The AM seedlings also exhibited significantly higher Cu/Zn-SOD and Mn-SOD activities than the non-AM seedlings under DS but not under WW, which are triggered by higher CaM levels in AM plants on the basis of correlation studies.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture and Gardening/Institute of Root Biology, Yangtze University Jingzhou, China.

ABSTRACT
Trifoliate orange [Poncirus trifoliata (L) Raf.] is considered highly arbuscular mycorrhizal (AM) dependent for growth responses through a series of signal transductions in form of various physiological responses. The proposed study was carried out to evaluate the effect of an AM fungus (Funneliformis mosseae) on growth, antioxidant enzyme (catalase, CAT; superoxide dismutase, SOD) activities, leaf relative water content (RWC), calmodulin (CaM), superoxide anion ([Formula: see text]), and hydrogen peroxide (H2O2) concentrations in leaves of the plants exposed to both well-watered (WW) and drought stress (DS) conditions. A 58-day of DS significantly decreased mycorrhizal colonization by 60% than WW. Compared to non-AM seedlings, AM seedlings displayed significantly higher shoot morphological properties (plant height, stem diameter, and leaf number), biomass production (shoot and root fresh weight) and leaf RWC, regardless of soil water status. AM inoculation significantly increased CaM and soluble protein concentrations and CAT activity, whereas significantly decreased [Formula: see text] and H2O2 concentration under both WW and DS conditions. The AM seedlings also exhibited significantly higher Cu/Zn-SOD and Mn-SOD activities than the non-AM seedlings under DS but not under WW, which are triggered by higher CaM levels in AM plants on the basis of correlation studies. Further, the negative correlation of Cu/Zn-SOD and Mn-SOD activities with [Formula: see text] and H2O2 concentration showed the DS-induced ROS scavenging ability of CaM mediated SODs under mycorrhization. Our results demonstrated that AM-inoculation elevated the synthesis of CaM in leaves and up-regulated activities of the antioxidant enzymes, thereby, repairing the possible oxidative damage to plants by lowering the ROS accumulation under DS condition.

No MeSH data available.


Related in: MedlinePlus