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Mycorrhizal-induced calmodulin mediated changes in antioxidant enzymes and growth response of drought-stressed trifoliate orange.

Huang YM, Srivastava AK, Zou YN, Ni QD, Han Y, Wu QS - Front Microbiol (2014)

Bottom Line: A 58-day of DS significantly decreased mycorrhizal colonization by 60% than WW.Compared to non-AM seedlings, AM seedlings displayed significantly higher shoot morphological properties (plant height, stem diameter, and leaf number), biomass production (shoot and root fresh weight) and leaf RWC, regardless of soil water status.The AM seedlings also exhibited significantly higher Cu/Zn-SOD and Mn-SOD activities than the non-AM seedlings under DS but not under WW, which are triggered by higher CaM levels in AM plants on the basis of correlation studies.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture and Gardening/Institute of Root Biology, Yangtze University Jingzhou, China.

ABSTRACT
Trifoliate orange [Poncirus trifoliata (L) Raf.] is considered highly arbuscular mycorrhizal (AM) dependent for growth responses through a series of signal transductions in form of various physiological responses. The proposed study was carried out to evaluate the effect of an AM fungus (Funneliformis mosseae) on growth, antioxidant enzyme (catalase, CAT; superoxide dismutase, SOD) activities, leaf relative water content (RWC), calmodulin (CaM), superoxide anion ([Formula: see text]), and hydrogen peroxide (H2O2) concentrations in leaves of the plants exposed to both well-watered (WW) and drought stress (DS) conditions. A 58-day of DS significantly decreased mycorrhizal colonization by 60% than WW. Compared to non-AM seedlings, AM seedlings displayed significantly higher shoot morphological properties (plant height, stem diameter, and leaf number), biomass production (shoot and root fresh weight) and leaf RWC, regardless of soil water status. AM inoculation significantly increased CaM and soluble protein concentrations and CAT activity, whereas significantly decreased [Formula: see text] and H2O2 concentration under both WW and DS conditions. The AM seedlings also exhibited significantly higher Cu/Zn-SOD and Mn-SOD activities than the non-AM seedlings under DS but not under WW, which are triggered by higher CaM levels in AM plants on the basis of correlation studies. Further, the negative correlation of Cu/Zn-SOD and Mn-SOD activities with [Formula: see text] and H2O2 concentration showed the DS-induced ROS scavenging ability of CaM mediated SODs under mycorrhization. Our results demonstrated that AM-inoculation elevated the synthesis of CaM in leaves and up-regulated activities of the antioxidant enzymes, thereby, repairing the possible oxidative damage to plants by lowering the ROS accumulation under DS condition.

No MeSH data available.


Related in: MedlinePlus

Root AM colonization of trifoliate orange seedlings by Funneliformis mosseae under well-watered (WW) and drought stress (DS) conditions. Data (means ± SD, n = 4) followed by different letters above the bars among treatments indicate significant differences at the 5% level.
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Figure 1: Root AM colonization of trifoliate orange seedlings by Funneliformis mosseae under well-watered (WW) and drought stress (DS) conditions. Data (means ± SD, n = 4) followed by different letters above the bars among treatments indicate significant differences at the 5% level.

Mentions: Vigor of plant growth depends upon the magnitude of root colonization as a result of AMF inoculation. Inoculation with F. mosseae induced varying magnitude of root colonization in trifoliate orange seedlings under both WW and DS conditions. However, root colonization under DS conditions, was observed only 31% compared to 77% under WW conditions (Figure 1). Mycorrhizal colonization was observed significantly dependent upon interaction effect of water status and AMF (Table 1). Moreover, the 58-day DS treatment significantly reduced the root colonization of trifoliate oranges by AMF. The decrease of root colonization under DS is reported in a wide range of crops (Wu et al., 2013), since spore germination and hyphal spread are strongly dependent on soil water status (Huang et al., 2011).


Mycorrhizal-induced calmodulin mediated changes in antioxidant enzymes and growth response of drought-stressed trifoliate orange.

Huang YM, Srivastava AK, Zou YN, Ni QD, Han Y, Wu QS - Front Microbiol (2014)

Root AM colonization of trifoliate orange seedlings by Funneliformis mosseae under well-watered (WW) and drought stress (DS) conditions. Data (means ± SD, n = 4) followed by different letters above the bars among treatments indicate significant differences at the 5% level.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4257356&req=5

Figure 1: Root AM colonization of trifoliate orange seedlings by Funneliformis mosseae under well-watered (WW) and drought stress (DS) conditions. Data (means ± SD, n = 4) followed by different letters above the bars among treatments indicate significant differences at the 5% level.
Mentions: Vigor of plant growth depends upon the magnitude of root colonization as a result of AMF inoculation. Inoculation with F. mosseae induced varying magnitude of root colonization in trifoliate orange seedlings under both WW and DS conditions. However, root colonization under DS conditions, was observed only 31% compared to 77% under WW conditions (Figure 1). Mycorrhizal colonization was observed significantly dependent upon interaction effect of water status and AMF (Table 1). Moreover, the 58-day DS treatment significantly reduced the root colonization of trifoliate oranges by AMF. The decrease of root colonization under DS is reported in a wide range of crops (Wu et al., 2013), since spore germination and hyphal spread are strongly dependent on soil water status (Huang et al., 2011).

Bottom Line: A 58-day of DS significantly decreased mycorrhizal colonization by 60% than WW.Compared to non-AM seedlings, AM seedlings displayed significantly higher shoot morphological properties (plant height, stem diameter, and leaf number), biomass production (shoot and root fresh weight) and leaf RWC, regardless of soil water status.The AM seedlings also exhibited significantly higher Cu/Zn-SOD and Mn-SOD activities than the non-AM seedlings under DS but not under WW, which are triggered by higher CaM levels in AM plants on the basis of correlation studies.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture and Gardening/Institute of Root Biology, Yangtze University Jingzhou, China.

ABSTRACT
Trifoliate orange [Poncirus trifoliata (L) Raf.] is considered highly arbuscular mycorrhizal (AM) dependent for growth responses through a series of signal transductions in form of various physiological responses. The proposed study was carried out to evaluate the effect of an AM fungus (Funneliformis mosseae) on growth, antioxidant enzyme (catalase, CAT; superoxide dismutase, SOD) activities, leaf relative water content (RWC), calmodulin (CaM), superoxide anion ([Formula: see text]), and hydrogen peroxide (H2O2) concentrations in leaves of the plants exposed to both well-watered (WW) and drought stress (DS) conditions. A 58-day of DS significantly decreased mycorrhizal colonization by 60% than WW. Compared to non-AM seedlings, AM seedlings displayed significantly higher shoot morphological properties (plant height, stem diameter, and leaf number), biomass production (shoot and root fresh weight) and leaf RWC, regardless of soil water status. AM inoculation significantly increased CaM and soluble protein concentrations and CAT activity, whereas significantly decreased [Formula: see text] and H2O2 concentration under both WW and DS conditions. The AM seedlings also exhibited significantly higher Cu/Zn-SOD and Mn-SOD activities than the non-AM seedlings under DS but not under WW, which are triggered by higher CaM levels in AM plants on the basis of correlation studies. Further, the negative correlation of Cu/Zn-SOD and Mn-SOD activities with [Formula: see text] and H2O2 concentration showed the DS-induced ROS scavenging ability of CaM mediated SODs under mycorrhization. Our results demonstrated that AM-inoculation elevated the synthesis of CaM in leaves and up-regulated activities of the antioxidant enzymes, thereby, repairing the possible oxidative damage to plants by lowering the ROS accumulation under DS condition.

No MeSH data available.


Related in: MedlinePlus