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Neogenin expression is inversely associated with breast cancer grade in ex vivo.

Xing W, Li Q, Cao R, Xu Z - World J Surg Oncol (2014)

Bottom Line: Neogenin is closely related to the human tumor suppressor gene deleted in colorectal cancer and plays a role in mammary morphogenesis.The data showed that the neogenin protein was either strongly or moderately expressed in the cytoplasm of the distant non-cancerous cells.This study indicates that neogenin expression in breast cancer tissues is inversely associated with tumor grade.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China. eryaoqi@126.com.

ABSTRACT

Background: Neogenin is closely related to the human tumor suppressor gene deleted in colorectal cancer and plays a role in mammary morphogenesis. This study aimed to assess neogenin expression in breast cancer for any clinically significant association.

Methods: A total of 54 breast cancer patients who underwent modified radical mastectomy were enrolled for immunohistochemical and quantitative real-time PCR analysis of neogenin expression in their cancerous breast tissues in comparison to matching distant non-cancerous tissues.

Results: The data showed that the neogenin protein was either strongly or moderately expressed in the cytoplasm of the distant non-cancerous cells. In contrast, neogenin protein was either weakly or not expressed in the cytoplasm of 51/54 (94.4%) breast cancer cells, among which 13 breast cancer cases did not express neogenin protein at all (13/54, 24.1%). Similarly, levels of neogenin mRNA were significantly lower in breast cancer tissues than that of the matched distant non-cancerous tissues (51/54, 94.4%). Neogenin expression was inversely associated with breast cancer grade; that is, grade III breast cancer expressed much less neogenin than grade I-II (P<0.05).

Conclusions: This study indicates that neogenin expression in breast cancer tissues is inversely associated with tumor grade.

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Association betweenneogeninexpression and clinicopathological parameters from breast cancer patients. (A) Expression of neogenin in histology grade II breast cancer tissue (n = 29). 200× magnification. (B)Neogenin expression in histology grade III breast cancer tissue (n = 24). 200× magnification. (C) Expression of neogenin mRNA in different histological grades of breast cancer.
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Fig2: Association betweenneogeninexpression and clinicopathological parameters from breast cancer patients. (A) Expression of neogenin in histology grade II breast cancer tissue (n = 29). 200× magnification. (B)Neogenin expression in histology grade III breast cancer tissue (n = 24). 200× magnification. (C) Expression of neogenin mRNA in different histological grades of breast cancer.

Mentions: We then associated the expression of neogenin protein with clinicopathological parameters from breast cancer patients (Table 1). The results show that neogenin expression is inversely associated with breast cancer grade; that is, grade III breast cancer expressed less neogenin than grade I-II (Figure 2A,B). Similar findings were observed with regard to neogenin mRNA expression (P < 0.05; Figure 2C). However, there was no association between neogenin expression and other clinicopathological parameters, such as tumor size, lymph node status, vascular invasion status, breast cancer subtype, TNM stage, and biomarker (ER, PR, HER-2, and Ki67).Table 1


Neogenin expression is inversely associated with breast cancer grade in ex vivo.

Xing W, Li Q, Cao R, Xu Z - World J Surg Oncol (2014)

Association betweenneogeninexpression and clinicopathological parameters from breast cancer patients. (A) Expression of neogenin in histology grade II breast cancer tissue (n = 29). 200× magnification. (B)Neogenin expression in histology grade III breast cancer tissue (n = 24). 200× magnification. (C) Expression of neogenin mRNA in different histological grades of breast cancer.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4256800&req=5

Fig2: Association betweenneogeninexpression and clinicopathological parameters from breast cancer patients. (A) Expression of neogenin in histology grade II breast cancer tissue (n = 29). 200× magnification. (B)Neogenin expression in histology grade III breast cancer tissue (n = 24). 200× magnification. (C) Expression of neogenin mRNA in different histological grades of breast cancer.
Mentions: We then associated the expression of neogenin protein with clinicopathological parameters from breast cancer patients (Table 1). The results show that neogenin expression is inversely associated with breast cancer grade; that is, grade III breast cancer expressed less neogenin than grade I-II (Figure 2A,B). Similar findings were observed with regard to neogenin mRNA expression (P < 0.05; Figure 2C). However, there was no association between neogenin expression and other clinicopathological parameters, such as tumor size, lymph node status, vascular invasion status, breast cancer subtype, TNM stage, and biomarker (ER, PR, HER-2, and Ki67).Table 1

Bottom Line: Neogenin is closely related to the human tumor suppressor gene deleted in colorectal cancer and plays a role in mammary morphogenesis.The data showed that the neogenin protein was either strongly or moderately expressed in the cytoplasm of the distant non-cancerous cells.This study indicates that neogenin expression in breast cancer tissues is inversely associated with tumor grade.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China. eryaoqi@126.com.

ABSTRACT

Background: Neogenin is closely related to the human tumor suppressor gene deleted in colorectal cancer and plays a role in mammary morphogenesis. This study aimed to assess neogenin expression in breast cancer for any clinically significant association.

Methods: A total of 54 breast cancer patients who underwent modified radical mastectomy were enrolled for immunohistochemical and quantitative real-time PCR analysis of neogenin expression in their cancerous breast tissues in comparison to matching distant non-cancerous tissues.

Results: The data showed that the neogenin protein was either strongly or moderately expressed in the cytoplasm of the distant non-cancerous cells. In contrast, neogenin protein was either weakly or not expressed in the cytoplasm of 51/54 (94.4%) breast cancer cells, among which 13 breast cancer cases did not express neogenin protein at all (13/54, 24.1%). Similarly, levels of neogenin mRNA were significantly lower in breast cancer tissues than that of the matched distant non-cancerous tissues (51/54, 94.4%). Neogenin expression was inversely associated with breast cancer grade; that is, grade III breast cancer expressed much less neogenin than grade I-II (P<0.05).

Conclusions: This study indicates that neogenin expression in breast cancer tissues is inversely associated with tumor grade.

Show MeSH
Related in: MedlinePlus