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MicroRNAs influence reproductive responses by females to male sex peptide in Drosophila melanogaster.

Fricke C, Green D, Smith D, Dalmay T, Chapman T - Genetics (2014)

Bottom Line: However, these effects interacted significantly with the genetic background of the miRNA-lacking females.No significant survival effects were observed in miRNA-lacking females housed continually with SP or control males.The results provide the first insight into the effects and importance of miRNAs in regulating postmating responses in females.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, University of East Anglia, Norwich Research Park, NR4 7TJ United Kingdom Institute for Evolution and Biodiversity, University of Muenster, 48149 Muenster, Germany.

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Related in: MedlinePlus

Survival curves for the miRNA mutant females (backcrossed into the w[Dah] genetic background) kept as virgins or continuously exposed to SP-lacking (SP0) or SP-transferring (SP+) control males throughout their lifetimes. Shown are the survival curves for the (A) mir-278D knockouts, (B) mir-279D, (C) mir-317D hypomorphic mutant females, and (D) w[Dah] controls. For each panel, the virgins are shown by the dotted lines, females mated with SP+ males by the solid lines, and females mated with SP0 males by the dashed lines.
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fig3: Survival curves for the miRNA mutant females (backcrossed into the w[Dah] genetic background) kept as virgins or continuously exposed to SP-lacking (SP0) or SP-transferring (SP+) control males throughout their lifetimes. Shown are the survival curves for the (A) mir-278D knockouts, (B) mir-279D, (C) mir-317D hypomorphic mutant females, and (D) w[Dah] controls. For each panel, the virgins are shown by the dotted lines, females mated with SP+ males by the solid lines, and females mated with SP0 males by the dashed lines.

Mentions: Virgin female genotypes differed significantly in survival (G23 = 23.36, P < 0.0001; Figure 3; Figure 4). Virgin mir-278D ko females had low early-life but higher late-life survival (Figure 3A). Virgin mir-317D females showed a steady decline in survival from day 20 onward, resulting in the lowest maximum lifespan (∼60 days compared to ∼70 days for the other female genotypes; Figure 3C). Continuous exposure to males significantly reduced female lifespan in comparison to virgin females in all groups (mating treatment: G22 = 623.48, P < 0.0001). However, this effect depended on female genotype (interaction: G26 = 38.53, P < 0.0001; female genotype: G23 = 11.92, P = 0.008; Table 2). Among mated females, female genotypes differed significantly in their responses to mating (G23 = 31.81, P < 0.0001; Figure 4, A and B), though SP receipt had no effect on female lifespan (male genotype: G21 = 0.12, P = 0.730; male × female genotype: G23 = 2.89, P = 0.408). Thus females differed in their susceptibility to male exposure, with mir-278D ko female lifespan (Figure 3A) being markedly reduced in comparison to the other genotypes tested (Figure 3; Figure 4); however, these effects were independent of SP receipt.


MicroRNAs influence reproductive responses by females to male sex peptide in Drosophila melanogaster.

Fricke C, Green D, Smith D, Dalmay T, Chapman T - Genetics (2014)

Survival curves for the miRNA mutant females (backcrossed into the w[Dah] genetic background) kept as virgins or continuously exposed to SP-lacking (SP0) or SP-transferring (SP+) control males throughout their lifetimes. Shown are the survival curves for the (A) mir-278D knockouts, (B) mir-279D, (C) mir-317D hypomorphic mutant females, and (D) w[Dah] controls. For each panel, the virgins are shown by the dotted lines, females mated with SP+ males by the solid lines, and females mated with SP0 males by the dashed lines.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4256774&req=5

fig3: Survival curves for the miRNA mutant females (backcrossed into the w[Dah] genetic background) kept as virgins or continuously exposed to SP-lacking (SP0) or SP-transferring (SP+) control males throughout their lifetimes. Shown are the survival curves for the (A) mir-278D knockouts, (B) mir-279D, (C) mir-317D hypomorphic mutant females, and (D) w[Dah] controls. For each panel, the virgins are shown by the dotted lines, females mated with SP+ males by the solid lines, and females mated with SP0 males by the dashed lines.
Mentions: Virgin female genotypes differed significantly in survival (G23 = 23.36, P < 0.0001; Figure 3; Figure 4). Virgin mir-278D ko females had low early-life but higher late-life survival (Figure 3A). Virgin mir-317D females showed a steady decline in survival from day 20 onward, resulting in the lowest maximum lifespan (∼60 days compared to ∼70 days for the other female genotypes; Figure 3C). Continuous exposure to males significantly reduced female lifespan in comparison to virgin females in all groups (mating treatment: G22 = 623.48, P < 0.0001). However, this effect depended on female genotype (interaction: G26 = 38.53, P < 0.0001; female genotype: G23 = 11.92, P = 0.008; Table 2). Among mated females, female genotypes differed significantly in their responses to mating (G23 = 31.81, P < 0.0001; Figure 4, A and B), though SP receipt had no effect on female lifespan (male genotype: G21 = 0.12, P = 0.730; male × female genotype: G23 = 2.89, P = 0.408). Thus females differed in their susceptibility to male exposure, with mir-278D ko female lifespan (Figure 3A) being markedly reduced in comparison to the other genotypes tested (Figure 3; Figure 4); however, these effects were independent of SP receipt.

Bottom Line: However, these effects interacted significantly with the genetic background of the miRNA-lacking females.No significant survival effects were observed in miRNA-lacking females housed continually with SP or control males.The results provide the first insight into the effects and importance of miRNAs in regulating postmating responses in females.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, University of East Anglia, Norwich Research Park, NR4 7TJ United Kingdom Institute for Evolution and Biodiversity, University of Muenster, 48149 Muenster, Germany.

Show MeSH
Related in: MedlinePlus