MicroRNAs influence reproductive responses by females to male sex peptide in Drosophila melanogaster.
Bottom Line: However, these effects interacted significantly with the genetic background of the miRNA-lacking females.No significant survival effects were observed in miRNA-lacking females housed continually with SP or control males.The results provide the first insight into the effects and importance of miRNAs in regulating postmating responses in females.
Affiliation: School of Biological Sciences, University of East Anglia, Norwich Research Park, NR4 7TJ United Kingdom Institute for Evolution and Biodiversity, University of Muenster, 48149 Muenster, Germany.Show MeSH
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Mentions: Virgin mutant and control females did not differ in their willingness to mate in their first mating (G22 = 1.67, P = 0.434) and this did not change over time (G21 = 2.84, P = 0.092; interaction = ns). There was a marginally nonsignificant difference in mating rate among the mated females (G22 = 5.33, P = 0.070) and the effect of time since the first mating on willingness to remate was similarly marginally nonsignificant (G21 = 3.15, P = 0.076). Receipt of SP significantly suppressed remating rate as expected (G21 = 84.26, P < 0.0001). There was a marginally nonsignificant interaction between female and male genotype (G22 = 5.68, P = 0.059, all other interactions = ns). This suggests that the effect of SP receipt on female sexual receptivity varied across the female genotypes tested. Across genotypes, females that received no SP remated at a rate similar to virgin females. However, SP was less efficient in suppressing remating in mir-317D females, whereas in mir-279D females, SP was equally effective in suppressing remating after 24 and 48 hr (Figure 2, A and E).
Affiliation: School of Biological Sciences, University of East Anglia, Norwich Research Park, NR4 7TJ United Kingdom Institute for Evolution and Biodiversity, University of Muenster, 48149 Muenster, Germany.