Aspirin influences megakaryocytic gene expression leading to up-regulation of multidrug resistance protein-4 in human platelets.
Bottom Line: We recently found that platelet MRP4 overexpression has a role in reducing aspirin action in patients after by-pass surgery.In DAMI cells, aspirin and WY14643 treatment induced a significant increase in MRP4 and PPARα expression.This work represents an innovative and attractive approach, useful both to identify patients less sensitive to aspirin and to improve pharmacological treatment in cardiovascular high-risk patients.
Affiliation: Department of Experimental Medicine, Sapienza University of Rome, Rome.Show MeSH
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Mentions: More importantly, we measured MRP4 and PPARα expression after discontinuation of the drug and we observed a significant decrease of MRP4 and PPARα expression in in vitro studies 2 days after suspension of aspirin treatment (WO 2 days). This effect occured because, 2 days after suspension, about 80% of DAMI cells analyzed were grown in the absence of aspirin. Decrease in MRP4 (Figure 3A) and PPARα (Figure 3B) expression became more evident after 5 and 7 days (WO 5 and WO 7 days).
Affiliation: Department of Experimental Medicine, Sapienza University of Rome, Rome.