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Aspirin influences megakaryocytic gene expression leading to up-regulation of multidrug resistance protein-4 in human platelets.

Massimi I, Guerriero R, Lotti LV, Lulli V, Borgognone A, Romani F, Barillà F, Gaudio C, Gabbianelli M, Frati L, Pulcinelli FM - Br J Clin Pharmacol (2014)

Bottom Line: We recently found that platelet MRP4 overexpression has a role in reducing aspirin action in patients after by-pass surgery.In DAMI cells, aspirin and WY14643 treatment induced a significant increase in MRP4 and PPARα expression.This work represents an innovative and attractive approach, useful both to identify patients less sensitive to aspirin and to improve pharmacological treatment in cardiovascular high-risk patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Sapienza University of Rome, Rome.

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Aspirin stimulates endogenous MRP4 protein expression in DAMI cells. Immunofluorescence analysis of MRP4 (red) in DAMI cells, in untreated (Ctr) or treated, for 48 h, with either 50 μm aspirin (ASA) or 1 μm PPARα agonist, WY14643 (WY). Nuclei are counterstained with DAPI (blue). All data shown are representative of three independent experiments
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fig02: Aspirin stimulates endogenous MRP4 protein expression in DAMI cells. Immunofluorescence analysis of MRP4 (red) in DAMI cells, in untreated (Ctr) or treated, for 48 h, with either 50 μm aspirin (ASA) or 1 μm PPARα agonist, WY14643 (WY). Nuclei are counterstained with DAPI (blue). All data shown are representative of three independent experiments

Mentions: In the same experimental conditions, immunofluorescence analysis confirmed a consistent MRP4 staining increase in cells treated with either aspirin or WY14643 in comparison with mock cells, that showed only a punctate labelling pattern (Figure 2A).


Aspirin influences megakaryocytic gene expression leading to up-regulation of multidrug resistance protein-4 in human platelets.

Massimi I, Guerriero R, Lotti LV, Lulli V, Borgognone A, Romani F, Barillà F, Gaudio C, Gabbianelli M, Frati L, Pulcinelli FM - Br J Clin Pharmacol (2014)

Aspirin stimulates endogenous MRP4 protein expression in DAMI cells. Immunofluorescence analysis of MRP4 (red) in DAMI cells, in untreated (Ctr) or treated, for 48 h, with either 50 μm aspirin (ASA) or 1 μm PPARα agonist, WY14643 (WY). Nuclei are counterstained with DAPI (blue). All data shown are representative of three independent experiments
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256623&req=5

fig02: Aspirin stimulates endogenous MRP4 protein expression in DAMI cells. Immunofluorescence analysis of MRP4 (red) in DAMI cells, in untreated (Ctr) or treated, for 48 h, with either 50 μm aspirin (ASA) or 1 μm PPARα agonist, WY14643 (WY). Nuclei are counterstained with DAPI (blue). All data shown are representative of three independent experiments
Mentions: In the same experimental conditions, immunofluorescence analysis confirmed a consistent MRP4 staining increase in cells treated with either aspirin or WY14643 in comparison with mock cells, that showed only a punctate labelling pattern (Figure 2A).

Bottom Line: We recently found that platelet MRP4 overexpression has a role in reducing aspirin action in patients after by-pass surgery.In DAMI cells, aspirin and WY14643 treatment induced a significant increase in MRP4 and PPARα expression.This work represents an innovative and attractive approach, useful both to identify patients less sensitive to aspirin and to improve pharmacological treatment in cardiovascular high-risk patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Sapienza University of Rome, Rome.

Show MeSH