Aspirin influences megakaryocytic gene expression leading to up-regulation of multidrug resistance protein-4 in human platelets.
Bottom Line: We recently found that platelet MRP4 overexpression has a role in reducing aspirin action in patients after by-pass surgery.In DAMI cells, aspirin and WY14643 treatment induced a significant increase in MRP4 and PPARα expression.This work represents an innovative and attractive approach, useful both to identify patients less sensitive to aspirin and to improve pharmacological treatment in cardiovascular high-risk patients.
Affiliation: Department of Experimental Medicine, Sapienza University of Rome, Rome.Show MeSH
Mentions: In the same experimental conditions, immunofluorescence analysis confirmed a consistent MRP4 staining increase in cells treated with either aspirin or WY14643 in comparison with mock cells, that showed only a punctate labelling pattern (Figure 2A).
Affiliation: Department of Experimental Medicine, Sapienza University of Rome, Rome.