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New insights on effects of a dietary supplement on oxidative and nitrosative stress in humans.

Nemzer BV, Fink N, Fink B - Food Sci Nutr (2014)

Bottom Line: In this clinical study, we demonstrated that administration of SPECTRA™ resulted in statistically significant long-term inhibition of mitochondrial and cellular ROS generation by as much as 17% as well as 3.5-times inhibition in extracellular NADPH system-dependent generation of O2 (-), and nearly complete inhibition of extracellular H2O2 formation.For the first time, we have measured synergetic, biological effects of a natural supplement on changes in OSM and cellular metabolic activity.The unique design and activity of the plant-based natural supplement, in combination with the newly developed and extended Vitality test, demonstrates the potential of using dietary supplements to modulate OSM and also opens the door to future research into the use of natural supplements for supporting optimal health.

View Article: PubMed Central - PubMed

Affiliation: VDF FutureCeuticals Inc. 2692 N State Rt. 1-17, Momence, Illinois, 60954 ; University of Illinois at Urbana-Champaign 1201 W. Gregory Dr, Urbana, Illinois, 61801.

ABSTRACT
The research community is generally agreed that maintenance of healthy levels of free radicals and related oxidants are important for good health. However, utilization of the "redox stress hypothesis" can provide us with concrete nutritional targets in order to better support and maintain "optimal health." Following this hypothesis we performed a crossover, double-blind, placebo-controlled, single-dose study on the effects of SPECTRA™, a dietary supplement, on oxidative stress markers (OSM) in human participants (n = 22). The measurement of OSM (ex vivo intra- and extracellular formation of reactive oxygen species (ROS, O2 (-), H2O2, OH(-)) in whole blood, respiratory activity of blood cells, as well as mitochondrial-dependent ROS formation, and respiratory activity), was performed using EPR spectrometer nOxyscan, spin probe CMH, and oxygen label NOX-15.1, respectively. Furthermore, we investigated the ability of SPECTRA™ to modulate ex vivo cellular inflammatory responses induced by stimulation with exogenous TNF-α and also followed changes in bioavailable NO concentrations. In this clinical study, we demonstrated that administration of SPECTRA™ resulted in statistically significant long-term inhibition of mitochondrial and cellular ROS generation by as much as 17% as well as 3.5-times inhibition in extracellular NADPH system-dependent generation of O2 (-), and nearly complete inhibition of extracellular H2O2 formation. This was reflected in more than two times inhibition of ex vivo cellular inflammatory response and also increases in bioavailable NO concentration. For the first time, we have measured synergetic, biological effects of a natural supplement on changes in OSM and cellular metabolic activity. The unique design and activity of the plant-based natural supplement, in combination with the newly developed and extended Vitality test, demonstrates the potential of using dietary supplements to modulate OSM and also opens the door to future research into the use of natural supplements for supporting optimal health.

No MeSH data available.


Related in: MedlinePlus

Inhibition of TNFα-induced “cellular inflammatory response” after single dose of SPECTRA™ in blood cells collected from human volunteers. This testing measured response of blood cells after chemical insult by stimulation with 40 ng/mL of exogenous human TNFα. As expected, this stimulation subsequently induced ROS (H2O2) formation. Levels of H2O2 in blood samples from the study subjects were analyzed using EPR spectrometer nOxyscan, nonmembrane permeable spin probe PPH (500 μmol/L). Blue column (control): 180 min after consumption of standard breakfast (bread roll with glass of water); red column (placebo): 180 min after consumption of standard breakfast and placebo capsule; and Green column (SPECTRA™): 180 minutes after consumption of standard breakfast and SPECTRA™ capsule. The accumulation of oxidized PP-radical was observed during 1 h incubation at 37C and 40 mmHg oxygen partial pressure. Data are mean ± SEM (n = 22), P < 0.01 versus placebo. Baseline and posttreatment levels of TNF-α were not measured. ROS, reactive oxygen species.
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fig07: Inhibition of TNFα-induced “cellular inflammatory response” after single dose of SPECTRA™ in blood cells collected from human volunteers. This testing measured response of blood cells after chemical insult by stimulation with 40 ng/mL of exogenous human TNFα. As expected, this stimulation subsequently induced ROS (H2O2) formation. Levels of H2O2 in blood samples from the study subjects were analyzed using EPR spectrometer nOxyscan, nonmembrane permeable spin probe PPH (500 μmol/L). Blue column (control): 180 min after consumption of standard breakfast (bread roll with glass of water); red column (placebo): 180 min after consumption of standard breakfast and placebo capsule; and Green column (SPECTRA™): 180 minutes after consumption of standard breakfast and SPECTRA™ capsule. The accumulation of oxidized PP-radical was observed during 1 h incubation at 37C and 40 mmHg oxygen partial pressure. Data are mean ± SEM (n = 22), P < 0.01 versus placebo. Baseline and posttreatment levels of TNF-α were not measured. ROS, reactive oxygen species.

Mentions: In order to provide more robust scientific proof on inhibition of peroxidase activities, which are linked to inflammatory response, we performed analysis of ex vivo changes in cellular ROS (almost hydrogen peroxide, H2O2) formation after a challenge by stimulation with externally introduced TNFα. TNFα is recognized as one of the key mediators of inflammation that is directly linked to ROS generation and apoptosis. We demonstrated significant inhibition of cellular response after administration of SPECTRA™ (Fig.7). Another example of the multifaceted effect of SPECTRA™, especially in terms of potential for support of cardiovascular health, is normalization of “nitrosative stress,” which may be evaluated based on analysis of bioavailable circulating NO concentration in vivo (Pisaneschi et al. 2012). Detection of circulating NO concentration in whole blood of participants after administration of SPECTRA™ showed significant increase in the level of NO (Fig.8).


New insights on effects of a dietary supplement on oxidative and nitrosative stress in humans.

Nemzer BV, Fink N, Fink B - Food Sci Nutr (2014)

Inhibition of TNFα-induced “cellular inflammatory response” after single dose of SPECTRA™ in blood cells collected from human volunteers. This testing measured response of blood cells after chemical insult by stimulation with 40 ng/mL of exogenous human TNFα. As expected, this stimulation subsequently induced ROS (H2O2) formation. Levels of H2O2 in blood samples from the study subjects were analyzed using EPR spectrometer nOxyscan, nonmembrane permeable spin probe PPH (500 μmol/L). Blue column (control): 180 min after consumption of standard breakfast (bread roll with glass of water); red column (placebo): 180 min after consumption of standard breakfast and placebo capsule; and Green column (SPECTRA™): 180 minutes after consumption of standard breakfast and SPECTRA™ capsule. The accumulation of oxidized PP-radical was observed during 1 h incubation at 37C and 40 mmHg oxygen partial pressure. Data are mean ± SEM (n = 22), P < 0.01 versus placebo. Baseline and posttreatment levels of TNF-α were not measured. ROS, reactive oxygen species.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256589&req=5

fig07: Inhibition of TNFα-induced “cellular inflammatory response” after single dose of SPECTRA™ in blood cells collected from human volunteers. This testing measured response of blood cells after chemical insult by stimulation with 40 ng/mL of exogenous human TNFα. As expected, this stimulation subsequently induced ROS (H2O2) formation. Levels of H2O2 in blood samples from the study subjects were analyzed using EPR spectrometer nOxyscan, nonmembrane permeable spin probe PPH (500 μmol/L). Blue column (control): 180 min after consumption of standard breakfast (bread roll with glass of water); red column (placebo): 180 min after consumption of standard breakfast and placebo capsule; and Green column (SPECTRA™): 180 minutes after consumption of standard breakfast and SPECTRA™ capsule. The accumulation of oxidized PP-radical was observed during 1 h incubation at 37C and 40 mmHg oxygen partial pressure. Data are mean ± SEM (n = 22), P < 0.01 versus placebo. Baseline and posttreatment levels of TNF-α were not measured. ROS, reactive oxygen species.
Mentions: In order to provide more robust scientific proof on inhibition of peroxidase activities, which are linked to inflammatory response, we performed analysis of ex vivo changes in cellular ROS (almost hydrogen peroxide, H2O2) formation after a challenge by stimulation with externally introduced TNFα. TNFα is recognized as one of the key mediators of inflammation that is directly linked to ROS generation and apoptosis. We demonstrated significant inhibition of cellular response after administration of SPECTRA™ (Fig.7). Another example of the multifaceted effect of SPECTRA™, especially in terms of potential for support of cardiovascular health, is normalization of “nitrosative stress,” which may be evaluated based on analysis of bioavailable circulating NO concentration in vivo (Pisaneschi et al. 2012). Detection of circulating NO concentration in whole blood of participants after administration of SPECTRA™ showed significant increase in the level of NO (Fig.8).

Bottom Line: In this clinical study, we demonstrated that administration of SPECTRA™ resulted in statistically significant long-term inhibition of mitochondrial and cellular ROS generation by as much as 17% as well as 3.5-times inhibition in extracellular NADPH system-dependent generation of O2 (-), and nearly complete inhibition of extracellular H2O2 formation.For the first time, we have measured synergetic, biological effects of a natural supplement on changes in OSM and cellular metabolic activity.The unique design and activity of the plant-based natural supplement, in combination with the newly developed and extended Vitality test, demonstrates the potential of using dietary supplements to modulate OSM and also opens the door to future research into the use of natural supplements for supporting optimal health.

View Article: PubMed Central - PubMed

Affiliation: VDF FutureCeuticals Inc. 2692 N State Rt. 1-17, Momence, Illinois, 60954 ; University of Illinois at Urbana-Champaign 1201 W. Gregory Dr, Urbana, Illinois, 61801.

ABSTRACT
The research community is generally agreed that maintenance of healthy levels of free radicals and related oxidants are important for good health. However, utilization of the "redox stress hypothesis" can provide us with concrete nutritional targets in order to better support and maintain "optimal health." Following this hypothesis we performed a crossover, double-blind, placebo-controlled, single-dose study on the effects of SPECTRA™, a dietary supplement, on oxidative stress markers (OSM) in human participants (n = 22). The measurement of OSM (ex vivo intra- and extracellular formation of reactive oxygen species (ROS, O2 (-), H2O2, OH(-)) in whole blood, respiratory activity of blood cells, as well as mitochondrial-dependent ROS formation, and respiratory activity), was performed using EPR spectrometer nOxyscan, spin probe CMH, and oxygen label NOX-15.1, respectively. Furthermore, we investigated the ability of SPECTRA™ to modulate ex vivo cellular inflammatory responses induced by stimulation with exogenous TNF-α and also followed changes in bioavailable NO concentrations. In this clinical study, we demonstrated that administration of SPECTRA™ resulted in statistically significant long-term inhibition of mitochondrial and cellular ROS generation by as much as 17% as well as 3.5-times inhibition in extracellular NADPH system-dependent generation of O2 (-), and nearly complete inhibition of extracellular H2O2 formation. This was reflected in more than two times inhibition of ex vivo cellular inflammatory response and also increases in bioavailable NO concentration. For the first time, we have measured synergetic, biological effects of a natural supplement on changes in OSM and cellular metabolic activity. The unique design and activity of the plant-based natural supplement, in combination with the newly developed and extended Vitality test, demonstrates the potential of using dietary supplements to modulate OSM and also opens the door to future research into the use of natural supplements for supporting optimal health.

No MeSH data available.


Related in: MedlinePlus