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Phytochemical screening and protective effects of Trifolium alexandrinum (L.) against free radical-induced stress in rats.

Shah AS, Ahmed M, Alkreathy HM, Khan MR, Khan RA, Khan S - Food Sci Nutr (2014)

Bottom Line: Results of in vitro study revealed significant (P < 0.05) antioxidant effects.Supplementation of TAME at 200 mg/kg b.w. for 2 weeks significantly improved activities of antioxidant enzymes and reduced TBARS formation.Antioxidant and nephroprotective effects of TAME are associated with its polyphenolic constituents.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, University of Science and Technology Bannu, KPK, Pakistan.

ABSTRACT
Trifolium alexandrinum is traditionally used in various human ailments, including renal dysfunctions. The present experiment was designed to investigate antioxidant and nephroprotective effect of T. alexandrinum methanolic extract (TAME) against CCl4-induced oxidative stress in albino rats. Results of in vitro study revealed significant (P < 0.05) antioxidant effects. The ameliorative role of TAME was also examined by investigating the level of antioxidant enzymes catalase (CAT), peroxidase (POD), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), nonenzymatic antioxidant viz; reduced glutathione contents (GSH) and lipid peroxidation products (TBARS) in the renal tissue homogenate in CCl4-treated rats. The intraperitoneal injection of 1 mL/kg b.w. CCl4 caused a significant depletion in the activity antioxidant enzymes and increased the TBARS contents. Supplementation of TAME at 200 mg/kg b.w. for 2 weeks significantly improved activities of antioxidant enzymes and reduced TBARS formation. Co-treatment of TAME also presented significant protection in maintaining renal urine and serum markers. Antioxidant and nephroprotective effects of TAME are associated with its polyphenolic constituents.

No MeSH data available.


Related in: MedlinePlus

In vitro antioxidant activity of TAME and ascorbic acid (1: 5 μg/mL; 2: 10 μg/mL; 3: 50 μg/mL; 4: 100 μg/mL).
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fig02: In vitro antioxidant activity of TAME and ascorbic acid (1: 5 μg/mL; 2: 10 μg/mL; 3: 50 μg/mL; 4: 100 μg/mL).

Mentions: In this part of study the antioxidant potential of TAME was investigated as a part of our efforts to find out pharmacological level of fruit. Results obtained in this context are revealed that scavenging of free radicals is dose dependent as shown in Figure2.


Phytochemical screening and protective effects of Trifolium alexandrinum (L.) against free radical-induced stress in rats.

Shah AS, Ahmed M, Alkreathy HM, Khan MR, Khan RA, Khan S - Food Sci Nutr (2014)

In vitro antioxidant activity of TAME and ascorbic acid (1: 5 μg/mL; 2: 10 μg/mL; 3: 50 μg/mL; 4: 100 μg/mL).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256581&req=5

fig02: In vitro antioxidant activity of TAME and ascorbic acid (1: 5 μg/mL; 2: 10 μg/mL; 3: 50 μg/mL; 4: 100 μg/mL).
Mentions: In this part of study the antioxidant potential of TAME was investigated as a part of our efforts to find out pharmacological level of fruit. Results obtained in this context are revealed that scavenging of free radicals is dose dependent as shown in Figure2.

Bottom Line: Results of in vitro study revealed significant (P < 0.05) antioxidant effects.Supplementation of TAME at 200 mg/kg b.w. for 2 weeks significantly improved activities of antioxidant enzymes and reduced TBARS formation.Antioxidant and nephroprotective effects of TAME are associated with its polyphenolic constituents.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, University of Science and Technology Bannu, KPK, Pakistan.

ABSTRACT
Trifolium alexandrinum is traditionally used in various human ailments, including renal dysfunctions. The present experiment was designed to investigate antioxidant and nephroprotective effect of T. alexandrinum methanolic extract (TAME) against CCl4-induced oxidative stress in albino rats. Results of in vitro study revealed significant (P < 0.05) antioxidant effects. The ameliorative role of TAME was also examined by investigating the level of antioxidant enzymes catalase (CAT), peroxidase (POD), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), nonenzymatic antioxidant viz; reduced glutathione contents (GSH) and lipid peroxidation products (TBARS) in the renal tissue homogenate in CCl4-treated rats. The intraperitoneal injection of 1 mL/kg b.w. CCl4 caused a significant depletion in the activity antioxidant enzymes and increased the TBARS contents. Supplementation of TAME at 200 mg/kg b.w. for 2 weeks significantly improved activities of antioxidant enzymes and reduced TBARS formation. Co-treatment of TAME also presented significant protection in maintaining renal urine and serum markers. Antioxidant and nephroprotective effects of TAME are associated with its polyphenolic constituents.

No MeSH data available.


Related in: MedlinePlus