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Anti-inflammatory effects of a casein hydrolysate and its peptide-enriched fractions on TNFα-challenged Caco-2 cells and LPS-challenged porcine colonic explants.

Mukhopadhya A, Noronha N, Bahar B, Ryan MT, Murray BA, Kelly PM, O'Loughlin IB, O'Doherty JV, Sweeney T - Food Sci Nutr (2014)

Bottom Line: Bioactive milk peptides are reported to illicit a range of physiological benefits and have been proposed as potential functional food ingredients.While dexamethasone reduced the IL-8 concentration by 41.6%, the 1 kDaR and 1 kDaP fractions reduced IL-8 by 68.7% and 66.1%, respectively, relative to TNFα-stimulated Caco-2 cells (P < 0.05).In the ex vivo system, only the 1 kDaR fraction elicited a decrease inIL1-α,IL1-β,IL-8,TGF-β andIL-10 expression (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: School of Veterinary Medicine, UCD Dublin, Ireland ; Food for Health Ireland (FHI), UCD Belfield, Dublin, Ireland.

ABSTRACT
Bioactive milk peptides are reported to illicit a range of physiological benefits and have been proposed as potential functional food ingredients. The objective of this study was to characterize the anti-inflammatory properties of sodium caseinate (NaCAS), its enzyme hydrolysate (EH) and peptide-enriched fractions (5 kDa retentate [R], 1 kDaR and 1 kDa permeate [P]), both in vitro using a Caco-2 cell line, and also ex vivo using a porcine colonic tissue explant system. Caco-2 cells were stimulated with tumour necrosis factor alpha (TNFα) and co-treated with casein hydrolysates for 24 h. Following this, interleukin (IL)-8 concentrations in the supernatant were measured using enzyme-linked immunosorbent assay. Porcine colonic tissue was stimulated with lipopolysaccharide and co-treated with casein hydrolysates for 3 h. The expression of a panel of inflammatory cytokines was measured using qPCR. While dexamethasone reduced the IL-8 concentration by 41.6%, the 1 kDaR and 1 kDaP fractions reduced IL-8 by 68.7% and 66.1%, respectively, relative to TNFα-stimulated Caco-2 cells (P < 0.05). In the ex vivo system, only the 1 kDaR fraction elicited a decrease inIL1-α,IL1-β,IL-8,TGF-β andIL-10 expression (P < 0.05). This study provides evidence that the bioactive peptides present in the 1 kDaR fraction of the NaCAS hydrolysate possess anti-inflammatory properties in vitro and ex vivo. Further in vivo analysis of the anti-inflammatory properties of the 1 kDaR is proposed.

No MeSH data available.


Related in: MedlinePlus

Difference in expression of a selected panel of cytokine genes between unchallenged and Lipopolysaccharides (LPS)-challenged ex vivo colonic tissues. The red dots represent significant upregulated genes (P < 0.05), the blue dot represents significant downregulated gene (P < 0.05) and black dots represent genes not affected by LPS (P > 0.05).
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fig02: Difference in expression of a selected panel of cytokine genes between unchallenged and Lipopolysaccharides (LPS)-challenged ex vivo colonic tissues. The red dots represent significant upregulated genes (P < 0.05), the blue dot represents significant downregulated gene (P < 0.05) and black dots represent genes not affected by LPS (P > 0.05).

Mentions: The effect of LPS challenge on the expression of a selected panel of cytokine genes from ex vivo colonic tissues is presented in Figure2. The LPS-challenged porcine colonic tissues were associated with increases in the normalized relative quantities ofIL1-α (0.424 vs. 0.064 ± 0.091,P < 0.05), IL1-β (0.442 vs. 0.062 ± 0.102,P < 0.01), IL-8 (0.212 vs. 0.064 ± 0.055,P < 0.05) andTNFα (0.234 vs. 0.024 ± 0.037,P < 0.001) compared to unchallenged colonic tissues. LPS-challenged tissues were also associated with a decrease inIFN-γ expression relative to unchallenged tissues (0.082 vs. 0.644 ± 0.047,P < 0.001). LPS had no effect onIL-6,IL-10,IL-17 andTGF-β expression, whileIL-4,IL-21 andFOXP3 expression was nondetectable in the colonic tissues used in this study.


Anti-inflammatory effects of a casein hydrolysate and its peptide-enriched fractions on TNFα-challenged Caco-2 cells and LPS-challenged porcine colonic explants.

Mukhopadhya A, Noronha N, Bahar B, Ryan MT, Murray BA, Kelly PM, O'Loughlin IB, O'Doherty JV, Sweeney T - Food Sci Nutr (2014)

Difference in expression of a selected panel of cytokine genes between unchallenged and Lipopolysaccharides (LPS)-challenged ex vivo colonic tissues. The red dots represent significant upregulated genes (P < 0.05), the blue dot represents significant downregulated gene (P < 0.05) and black dots represent genes not affected by LPS (P > 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256577&req=5

fig02: Difference in expression of a selected panel of cytokine genes between unchallenged and Lipopolysaccharides (LPS)-challenged ex vivo colonic tissues. The red dots represent significant upregulated genes (P < 0.05), the blue dot represents significant downregulated gene (P < 0.05) and black dots represent genes not affected by LPS (P > 0.05).
Mentions: The effect of LPS challenge on the expression of a selected panel of cytokine genes from ex vivo colonic tissues is presented in Figure2. The LPS-challenged porcine colonic tissues were associated with increases in the normalized relative quantities ofIL1-α (0.424 vs. 0.064 ± 0.091,P < 0.05), IL1-β (0.442 vs. 0.062 ± 0.102,P < 0.01), IL-8 (0.212 vs. 0.064 ± 0.055,P < 0.05) andTNFα (0.234 vs. 0.024 ± 0.037,P < 0.001) compared to unchallenged colonic tissues. LPS-challenged tissues were also associated with a decrease inIFN-γ expression relative to unchallenged tissues (0.082 vs. 0.644 ± 0.047,P < 0.001). LPS had no effect onIL-6,IL-10,IL-17 andTGF-β expression, whileIL-4,IL-21 andFOXP3 expression was nondetectable in the colonic tissues used in this study.

Bottom Line: Bioactive milk peptides are reported to illicit a range of physiological benefits and have been proposed as potential functional food ingredients.While dexamethasone reduced the IL-8 concentration by 41.6%, the 1 kDaR and 1 kDaP fractions reduced IL-8 by 68.7% and 66.1%, respectively, relative to TNFα-stimulated Caco-2 cells (P < 0.05).In the ex vivo system, only the 1 kDaR fraction elicited a decrease inIL1-α,IL1-β,IL-8,TGF-β andIL-10 expression (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: School of Veterinary Medicine, UCD Dublin, Ireland ; Food for Health Ireland (FHI), UCD Belfield, Dublin, Ireland.

ABSTRACT
Bioactive milk peptides are reported to illicit a range of physiological benefits and have been proposed as potential functional food ingredients. The objective of this study was to characterize the anti-inflammatory properties of sodium caseinate (NaCAS), its enzyme hydrolysate (EH) and peptide-enriched fractions (5 kDa retentate [R], 1 kDaR and 1 kDa permeate [P]), both in vitro using a Caco-2 cell line, and also ex vivo using a porcine colonic tissue explant system. Caco-2 cells were stimulated with tumour necrosis factor alpha (TNFα) and co-treated with casein hydrolysates for 24 h. Following this, interleukin (IL)-8 concentrations in the supernatant were measured using enzyme-linked immunosorbent assay. Porcine colonic tissue was stimulated with lipopolysaccharide and co-treated with casein hydrolysates for 3 h. The expression of a panel of inflammatory cytokines was measured using qPCR. While dexamethasone reduced the IL-8 concentration by 41.6%, the 1 kDaR and 1 kDaP fractions reduced IL-8 by 68.7% and 66.1%, respectively, relative to TNFα-stimulated Caco-2 cells (P < 0.05). In the ex vivo system, only the 1 kDaR fraction elicited a decrease inIL1-α,IL1-β,IL-8,TGF-β andIL-10 expression (P < 0.05). This study provides evidence that the bioactive peptides present in the 1 kDaR fraction of the NaCAS hydrolysate possess anti-inflammatory properties in vitro and ex vivo. Further in vivo analysis of the anti-inflammatory properties of the 1 kDaR is proposed.

No MeSH data available.


Related in: MedlinePlus