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Anti-inflammatory effects of a casein hydrolysate and its peptide-enriched fractions on TNFα-challenged Caco-2 cells and LPS-challenged porcine colonic explants.

Mukhopadhya A, Noronha N, Bahar B, Ryan MT, Murray BA, Kelly PM, O'Loughlin IB, O'Doherty JV, Sweeney T - Food Sci Nutr (2014)

Bottom Line: Bioactive milk peptides are reported to illicit a range of physiological benefits and have been proposed as potential functional food ingredients.While dexamethasone reduced the IL-8 concentration by 41.6%, the 1 kDaR and 1 kDaP fractions reduced IL-8 by 68.7% and 66.1%, respectively, relative to TNFα-stimulated Caco-2 cells (P < 0.05).In the ex vivo system, only the 1 kDaR fraction elicited a decrease inIL1-α,IL1-β,IL-8,TGF-β andIL-10 expression (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: School of Veterinary Medicine, UCD Dublin, Ireland ; Food for Health Ireland (FHI), UCD Belfield, Dublin, Ireland.

ABSTRACT
Bioactive milk peptides are reported to illicit a range of physiological benefits and have been proposed as potential functional food ingredients. The objective of this study was to characterize the anti-inflammatory properties of sodium caseinate (NaCAS), its enzyme hydrolysate (EH) and peptide-enriched fractions (5 kDa retentate [R], 1 kDaR and 1 kDa permeate [P]), both in vitro using a Caco-2 cell line, and also ex vivo using a porcine colonic tissue explant system. Caco-2 cells were stimulated with tumour necrosis factor alpha (TNFα) and co-treated with casein hydrolysates for 24 h. Following this, interleukin (IL)-8 concentrations in the supernatant were measured using enzyme-linked immunosorbent assay. Porcine colonic tissue was stimulated with lipopolysaccharide and co-treated with casein hydrolysates for 3 h. The expression of a panel of inflammatory cytokines was measured using qPCR. While dexamethasone reduced the IL-8 concentration by 41.6%, the 1 kDaR and 1 kDaP fractions reduced IL-8 by 68.7% and 66.1%, respectively, relative to TNFα-stimulated Caco-2 cells (P < 0.05). In the ex vivo system, only the 1 kDaR fraction elicited a decrease inIL1-α,IL1-β,IL-8,TGF-β andIL-10 expression (P < 0.05). This study provides evidence that the bioactive peptides present in the 1 kDaR fraction of the NaCAS hydrolysate possess anti-inflammatory properties in vitro and ex vivo. Further in vivo analysis of the anti-inflammatory properties of the 1 kDaR is proposed.

No MeSH data available.


Related in: MedlinePlus

Dose dependent reduction of IL-8 concentration by a milk hydrolysate series (NaCAS, EH, 5 kDaR, 1 kDaR and 1 kDaP). Each data point indicatesn = 3 ± standard error. The fully differentiated Caco-2 cells were challenged withTNFα and co-treated with milk hydrolysates for 24 h, cell lysate collected after 24 h and ELISA performed.
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fig01: Dose dependent reduction of IL-8 concentration by a milk hydrolysate series (NaCAS, EH, 5 kDaR, 1 kDaR and 1 kDaP). Each data point indicatesn = 3 ± standard error. The fully differentiated Caco-2 cells were challenged withTNFα and co-treated with milk hydrolysates for 24 h, cell lysate collected after 24 h and ELISA performed.

Mentions: The dose-dependent anti-inflammatory response of the casein hydrolysate and associated fractions and the optimum working concentrations are presented in Figure1. All the samples from this series were associated with a dose-dependent anti-inflammatory response based on IL-8 concentrations. The co-treatment of TNFα-stimulated Caco-2 cells with concentrations of 1 mg/mL and above was consistently associated with a substantial reduction in IL-8 concentration over the 24 h period.


Anti-inflammatory effects of a casein hydrolysate and its peptide-enriched fractions on TNFα-challenged Caco-2 cells and LPS-challenged porcine colonic explants.

Mukhopadhya A, Noronha N, Bahar B, Ryan MT, Murray BA, Kelly PM, O'Loughlin IB, O'Doherty JV, Sweeney T - Food Sci Nutr (2014)

Dose dependent reduction of IL-8 concentration by a milk hydrolysate series (NaCAS, EH, 5 kDaR, 1 kDaR and 1 kDaP). Each data point indicatesn = 3 ± standard error. The fully differentiated Caco-2 cells were challenged withTNFα and co-treated with milk hydrolysates for 24 h, cell lysate collected after 24 h and ELISA performed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256577&req=5

fig01: Dose dependent reduction of IL-8 concentration by a milk hydrolysate series (NaCAS, EH, 5 kDaR, 1 kDaR and 1 kDaP). Each data point indicatesn = 3 ± standard error. The fully differentiated Caco-2 cells were challenged withTNFα and co-treated with milk hydrolysates for 24 h, cell lysate collected after 24 h and ELISA performed.
Mentions: The dose-dependent anti-inflammatory response of the casein hydrolysate and associated fractions and the optimum working concentrations are presented in Figure1. All the samples from this series were associated with a dose-dependent anti-inflammatory response based on IL-8 concentrations. The co-treatment of TNFα-stimulated Caco-2 cells with concentrations of 1 mg/mL and above was consistently associated with a substantial reduction in IL-8 concentration over the 24 h period.

Bottom Line: Bioactive milk peptides are reported to illicit a range of physiological benefits and have been proposed as potential functional food ingredients.While dexamethasone reduced the IL-8 concentration by 41.6%, the 1 kDaR and 1 kDaP fractions reduced IL-8 by 68.7% and 66.1%, respectively, relative to TNFα-stimulated Caco-2 cells (P < 0.05).In the ex vivo system, only the 1 kDaR fraction elicited a decrease inIL1-α,IL1-β,IL-8,TGF-β andIL-10 expression (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: School of Veterinary Medicine, UCD Dublin, Ireland ; Food for Health Ireland (FHI), UCD Belfield, Dublin, Ireland.

ABSTRACT
Bioactive milk peptides are reported to illicit a range of physiological benefits and have been proposed as potential functional food ingredients. The objective of this study was to characterize the anti-inflammatory properties of sodium caseinate (NaCAS), its enzyme hydrolysate (EH) and peptide-enriched fractions (5 kDa retentate [R], 1 kDaR and 1 kDa permeate [P]), both in vitro using a Caco-2 cell line, and also ex vivo using a porcine colonic tissue explant system. Caco-2 cells were stimulated with tumour necrosis factor alpha (TNFα) and co-treated with casein hydrolysates for 24 h. Following this, interleukin (IL)-8 concentrations in the supernatant were measured using enzyme-linked immunosorbent assay. Porcine colonic tissue was stimulated with lipopolysaccharide and co-treated with casein hydrolysates for 3 h. The expression of a panel of inflammatory cytokines was measured using qPCR. While dexamethasone reduced the IL-8 concentration by 41.6%, the 1 kDaR and 1 kDaP fractions reduced IL-8 by 68.7% and 66.1%, respectively, relative to TNFα-stimulated Caco-2 cells (P < 0.05). In the ex vivo system, only the 1 kDaR fraction elicited a decrease inIL1-α,IL1-β,IL-8,TGF-β andIL-10 expression (P < 0.05). This study provides evidence that the bioactive peptides present in the 1 kDaR fraction of the NaCAS hydrolysate possess anti-inflammatory properties in vitro and ex vivo. Further in vivo analysis of the anti-inflammatory properties of the 1 kDaR is proposed.

No MeSH data available.


Related in: MedlinePlus