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Decrease of free radical concentrations in humans following consumption of a high antioxidant capacity natural product.

Nemzer B, Chang T, Xie Z, Pietrzkowski Z, Reyes T, Ou B - Food Sci Nutr (2014)

Bottom Line: Dihydrorhodamine-6G (DHR6G) is indiscriminate to the various free radicals found in humans, and therefore can be useful in quantifying total ROS in vivo.This method is both reliable and efficient for evaluating the efficacy of antioxidants against ROS in vivo.Our data indicate that eleven participants responded to the intake of Spectra™ by significant decreases of ROS concentrations.

View Article: PubMed Central - PubMed

Affiliation: VDF FutureCeuticals 2692 N State Rt. 1-17, Momence, Illinois, 60954 ; University of Illinois at Urbana-Champaign 1201 W. Gregory Dr, Urbana, Illinois, 61801.

ABSTRACT
ORAC and other in vitro methods have to date proved useful in measuring antioxidant potential in foods. In order to better understand the potential relationship between diet and free radical production/mitigation, an in vivo analytic method can provide new insight into directly measuring reactive oxidant species (ROS). Dihydrorhodamine-6G (DHR6G) is indiscriminate to the various free radicals found in humans, and therefore can be useful in quantifying total ROS in vivo. Our aim was to investigate whether the total ROS in human subjects can be quantified using DHR6G after intake of a blend of antioxidants-rich fruit and vegetable-based materials. Twelve participants were given 100 mg of a proprietary blend of fruit, vegetable, and herb powders and concentrates commercially marketed under the trade name "Spectra™". Blood samples were collected at 0, 60, 120 and 180 min and were subsequently tested for ROS in serum using DHR6G as a fluorescent probe. By quantifying this fluorescence, we were able to measure ROS concentrations in human blood. This method is both reliable and efficient for evaluating the efficacy of antioxidants against ROS in vivo. Our data indicate that eleven participants responded to the intake of Spectra™ by significant decreases of ROS concentrations.

No MeSH data available.


Related in: MedlinePlus

Standard curve of rhodamine 6G (0.406 to 26 μmol/L).
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fig04: Standard curve of rhodamine 6G (0.406 to 26 μmol/L).

Mentions: The DHR6G reacts with the radicals present in prepared samples regardless of species. The concentration of rhodamine 6G formed in the reaction with free radicals is calculated from a standard curve obtained by plotting the known rhodamine 6G concentration versus the fluorescent intensity. The linearity ranges from 0.406 to 26 μmol/L as shown in Figure4. Using this standard curve, the concentrations of rhodamine 6G formed during the reaction of free radicals with DHR-6G can be calculated. The stoichiometry between DHR-6G and free radicals is two. Thus,


Decrease of free radical concentrations in humans following consumption of a high antioxidant capacity natural product.

Nemzer B, Chang T, Xie Z, Pietrzkowski Z, Reyes T, Ou B - Food Sci Nutr (2014)

Standard curve of rhodamine 6G (0.406 to 26 μmol/L).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256568&req=5

fig04: Standard curve of rhodamine 6G (0.406 to 26 μmol/L).
Mentions: The DHR6G reacts with the radicals present in prepared samples regardless of species. The concentration of rhodamine 6G formed in the reaction with free radicals is calculated from a standard curve obtained by plotting the known rhodamine 6G concentration versus the fluorescent intensity. The linearity ranges from 0.406 to 26 μmol/L as shown in Figure4. Using this standard curve, the concentrations of rhodamine 6G formed during the reaction of free radicals with DHR-6G can be calculated. The stoichiometry between DHR-6G and free radicals is two. Thus,

Bottom Line: Dihydrorhodamine-6G (DHR6G) is indiscriminate to the various free radicals found in humans, and therefore can be useful in quantifying total ROS in vivo.This method is both reliable and efficient for evaluating the efficacy of antioxidants against ROS in vivo.Our data indicate that eleven participants responded to the intake of Spectra™ by significant decreases of ROS concentrations.

View Article: PubMed Central - PubMed

Affiliation: VDF FutureCeuticals 2692 N State Rt. 1-17, Momence, Illinois, 60954 ; University of Illinois at Urbana-Champaign 1201 W. Gregory Dr, Urbana, Illinois, 61801.

ABSTRACT
ORAC and other in vitro methods have to date proved useful in measuring antioxidant potential in foods. In order to better understand the potential relationship between diet and free radical production/mitigation, an in vivo analytic method can provide new insight into directly measuring reactive oxidant species (ROS). Dihydrorhodamine-6G (DHR6G) is indiscriminate to the various free radicals found in humans, and therefore can be useful in quantifying total ROS in vivo. Our aim was to investigate whether the total ROS in human subjects can be quantified using DHR6G after intake of a blend of antioxidants-rich fruit and vegetable-based materials. Twelve participants were given 100 mg of a proprietary blend of fruit, vegetable, and herb powders and concentrates commercially marketed under the trade name "Spectra™". Blood samples were collected at 0, 60, 120 and 180 min and were subsequently tested for ROS in serum using DHR6G as a fluorescent probe. By quantifying this fluorescence, we were able to measure ROS concentrations in human blood. This method is both reliable and efficient for evaluating the efficacy of antioxidants against ROS in vivo. Our data indicate that eleven participants responded to the intake of Spectra™ by significant decreases of ROS concentrations.

No MeSH data available.


Related in: MedlinePlus