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Decrease of free radical concentrations in humans following consumption of a high antioxidant capacity natural product.

Nemzer B, Chang T, Xie Z, Pietrzkowski Z, Reyes T, Ou B - Food Sci Nutr (2014)

Bottom Line: Dihydrorhodamine-6G (DHR6G) is indiscriminate to the various free radicals found in humans, and therefore can be useful in quantifying total ROS in vivo.This method is both reliable and efficient for evaluating the efficacy of antioxidants against ROS in vivo.Our data indicate that eleven participants responded to the intake of Spectra™ by significant decreases of ROS concentrations.

View Article: PubMed Central - PubMed

Affiliation: VDF FutureCeuticals 2692 N State Rt. 1-17, Momence, Illinois, 60954 ; University of Illinois at Urbana-Champaign 1201 W. Gregory Dr, Urbana, Illinois, 61801.

ABSTRACT
ORAC and other in vitro methods have to date proved useful in measuring antioxidant potential in foods. In order to better understand the potential relationship between diet and free radical production/mitigation, an in vivo analytic method can provide new insight into directly measuring reactive oxidant species (ROS). Dihydrorhodamine-6G (DHR6G) is indiscriminate to the various free radicals found in humans, and therefore can be useful in quantifying total ROS in vivo. Our aim was to investigate whether the total ROS in human subjects can be quantified using DHR6G after intake of a blend of antioxidants-rich fruit and vegetable-based materials. Twelve participants were given 100 mg of a proprietary blend of fruit, vegetable, and herb powders and concentrates commercially marketed under the trade name "Spectra™". Blood samples were collected at 0, 60, 120 and 180 min and were subsequently tested for ROS in serum using DHR6G as a fluorescent probe. By quantifying this fluorescence, we were able to measure ROS concentrations in human blood. This method is both reliable and efficient for evaluating the efficacy of antioxidants against ROS in vivo. Our data indicate that eleven participants responded to the intake of Spectra™ by significant decreases of ROS concentrations.

No MeSH data available.


Related in: MedlinePlus

DHR6G at 0.406 μmol/L was incubated with PBS buffer in a 96 plate well, and its fluorescence intensity was monitored for 24 h at 5-min intervals. Apparently, DHR6G was not auto-oxidized under these experimental conditions. DHR6G, dihydrorhodamine-6G.
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fig02: DHR6G at 0.406 μmol/L was incubated with PBS buffer in a 96 plate well, and its fluorescence intensity was monitored for 24 h at 5-min intervals. Apparently, DHR6G was not auto-oxidized under these experimental conditions. DHR6G, dihydrorhodamine-6G.

Mentions: Due to the fact that DHR6G is labeled as “air-sensitive,” we tested its stability when exposed to the air by incubating it in PBS buffer. As shown in Figure2, during the 24-h period, only an insignificant change of fluorescence intensity was observed. On the contrary, in the presence of serum extract, the fluorescence intensity changes were apparent and linearly increased in a time sensitive manner (Fig.1). Therefore, we conclude that, under these experimental conditions, air has little contribution to any oxidation of DHR6G; instead, the ROS in the samples should be responsible for the formation of rhodamine 6G. From the reaction mechanism, one DHR6G molecule reacts with two radical molecules in order to form rhodamine 6G. This finding greatly simplifies the quantitation of ROS in vivo (Fig.3).


Decrease of free radical concentrations in humans following consumption of a high antioxidant capacity natural product.

Nemzer B, Chang T, Xie Z, Pietrzkowski Z, Reyes T, Ou B - Food Sci Nutr (2014)

DHR6G at 0.406 μmol/L was incubated with PBS buffer in a 96 plate well, and its fluorescence intensity was monitored for 24 h at 5-min intervals. Apparently, DHR6G was not auto-oxidized under these experimental conditions. DHR6G, dihydrorhodamine-6G.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256568&req=5

fig02: DHR6G at 0.406 μmol/L was incubated with PBS buffer in a 96 plate well, and its fluorescence intensity was monitored for 24 h at 5-min intervals. Apparently, DHR6G was not auto-oxidized under these experimental conditions. DHR6G, dihydrorhodamine-6G.
Mentions: Due to the fact that DHR6G is labeled as “air-sensitive,” we tested its stability when exposed to the air by incubating it in PBS buffer. As shown in Figure2, during the 24-h period, only an insignificant change of fluorescence intensity was observed. On the contrary, in the presence of serum extract, the fluorescence intensity changes were apparent and linearly increased in a time sensitive manner (Fig.1). Therefore, we conclude that, under these experimental conditions, air has little contribution to any oxidation of DHR6G; instead, the ROS in the samples should be responsible for the formation of rhodamine 6G. From the reaction mechanism, one DHR6G molecule reacts with two radical molecules in order to form rhodamine 6G. This finding greatly simplifies the quantitation of ROS in vivo (Fig.3).

Bottom Line: Dihydrorhodamine-6G (DHR6G) is indiscriminate to the various free radicals found in humans, and therefore can be useful in quantifying total ROS in vivo.This method is both reliable and efficient for evaluating the efficacy of antioxidants against ROS in vivo.Our data indicate that eleven participants responded to the intake of Spectra™ by significant decreases of ROS concentrations.

View Article: PubMed Central - PubMed

Affiliation: VDF FutureCeuticals 2692 N State Rt. 1-17, Momence, Illinois, 60954 ; University of Illinois at Urbana-Champaign 1201 W. Gregory Dr, Urbana, Illinois, 61801.

ABSTRACT
ORAC and other in vitro methods have to date proved useful in measuring antioxidant potential in foods. In order to better understand the potential relationship between diet and free radical production/mitigation, an in vivo analytic method can provide new insight into directly measuring reactive oxidant species (ROS). Dihydrorhodamine-6G (DHR6G) is indiscriminate to the various free radicals found in humans, and therefore can be useful in quantifying total ROS in vivo. Our aim was to investigate whether the total ROS in human subjects can be quantified using DHR6G after intake of a blend of antioxidants-rich fruit and vegetable-based materials. Twelve participants were given 100 mg of a proprietary blend of fruit, vegetable, and herb powders and concentrates commercially marketed under the trade name "Spectra™". Blood samples were collected at 0, 60, 120 and 180 min and were subsequently tested for ROS in serum using DHR6G as a fluorescent probe. By quantifying this fluorescence, we were able to measure ROS concentrations in human blood. This method is both reliable and efficient for evaluating the efficacy of antioxidants against ROS in vivo. Our data indicate that eleven participants responded to the intake of Spectra™ by significant decreases of ROS concentrations.

No MeSH data available.


Related in: MedlinePlus