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Decrease of free radical concentrations in humans following consumption of a high antioxidant capacity natural product.

Nemzer B, Chang T, Xie Z, Pietrzkowski Z, Reyes T, Ou B - Food Sci Nutr (2014)

Bottom Line: Dihydrorhodamine-6G (DHR6G) is indiscriminate to the various free radicals found in humans, and therefore can be useful in quantifying total ROS in vivo.This method is both reliable and efficient for evaluating the efficacy of antioxidants against ROS in vivo.Our data indicate that eleven participants responded to the intake of Spectra™ by significant decreases of ROS concentrations.

View Article: PubMed Central - PubMed

Affiliation: VDF FutureCeuticals 2692 N State Rt. 1-17, Momence, Illinois, 60954 ; University of Illinois at Urbana-Champaign 1201 W. Gregory Dr, Urbana, Illinois, 61801.

ABSTRACT
ORAC and other in vitro methods have to date proved useful in measuring antioxidant potential in foods. In order to better understand the potential relationship between diet and free radical production/mitigation, an in vivo analytic method can provide new insight into directly measuring reactive oxidant species (ROS). Dihydrorhodamine-6G (DHR6G) is indiscriminate to the various free radicals found in humans, and therefore can be useful in quantifying total ROS in vivo. Our aim was to investigate whether the total ROS in human subjects can be quantified using DHR6G after intake of a blend of antioxidants-rich fruit and vegetable-based materials. Twelve participants were given 100 mg of a proprietary blend of fruit, vegetable, and herb powders and concentrates commercially marketed under the trade name "Spectra™". Blood samples were collected at 0, 60, 120 and 180 min and were subsequently tested for ROS in serum using DHR6G as a fluorescent probe. By quantifying this fluorescence, we were able to measure ROS concentrations in human blood. This method is both reliable and efficient for evaluating the efficacy of antioxidants against ROS in vivo. Our data indicate that eleven participants responded to the intake of Spectra™ by significant decreases of ROS concentrations.

No MeSH data available.


Related in: MedlinePlus

The changes in fluorescence intensity show that oxidation of DHR6G was significantly inhibited after intake of 100 mg Spectra (at 0 min). DHR6G, dihydrorhodamine-6G.
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fig01: The changes in fluorescence intensity show that oxidation of DHR6G was significantly inhibited after intake of 100 mg Spectra (at 0 min). DHR6G, dihydrorhodamine-6G.

Mentions: Originally, DHR6G was used in our previous study to detect and quantify smoke oxidants (Ou and Huang 2006). Through modification in the current study, DHR6G was able to be oxidized to rhodamine 6G during the course of incubation with the serum extract. Figure1 shows the measure of fluorescence intensity over the specified time period. It was observed that after 60 min of administration of 100 mg Spectra™, the rate of fluorescence intensity was significantly inhibited. This evidence suggests that Spectra™ was bioavailable in vivo and that the absorbed antioxidants neutralized endogenous free radicals.


Decrease of free radical concentrations in humans following consumption of a high antioxidant capacity natural product.

Nemzer B, Chang T, Xie Z, Pietrzkowski Z, Reyes T, Ou B - Food Sci Nutr (2014)

The changes in fluorescence intensity show that oxidation of DHR6G was significantly inhibited after intake of 100 mg Spectra (at 0 min). DHR6G, dihydrorhodamine-6G.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256568&req=5

fig01: The changes in fluorescence intensity show that oxidation of DHR6G was significantly inhibited after intake of 100 mg Spectra (at 0 min). DHR6G, dihydrorhodamine-6G.
Mentions: Originally, DHR6G was used in our previous study to detect and quantify smoke oxidants (Ou and Huang 2006). Through modification in the current study, DHR6G was able to be oxidized to rhodamine 6G during the course of incubation with the serum extract. Figure1 shows the measure of fluorescence intensity over the specified time period. It was observed that after 60 min of administration of 100 mg Spectra™, the rate of fluorescence intensity was significantly inhibited. This evidence suggests that Spectra™ was bioavailable in vivo and that the absorbed antioxidants neutralized endogenous free radicals.

Bottom Line: Dihydrorhodamine-6G (DHR6G) is indiscriminate to the various free radicals found in humans, and therefore can be useful in quantifying total ROS in vivo.This method is both reliable and efficient for evaluating the efficacy of antioxidants against ROS in vivo.Our data indicate that eleven participants responded to the intake of Spectra™ by significant decreases of ROS concentrations.

View Article: PubMed Central - PubMed

Affiliation: VDF FutureCeuticals 2692 N State Rt. 1-17, Momence, Illinois, 60954 ; University of Illinois at Urbana-Champaign 1201 W. Gregory Dr, Urbana, Illinois, 61801.

ABSTRACT
ORAC and other in vitro methods have to date proved useful in measuring antioxidant potential in foods. In order to better understand the potential relationship between diet and free radical production/mitigation, an in vivo analytic method can provide new insight into directly measuring reactive oxidant species (ROS). Dihydrorhodamine-6G (DHR6G) is indiscriminate to the various free radicals found in humans, and therefore can be useful in quantifying total ROS in vivo. Our aim was to investigate whether the total ROS in human subjects can be quantified using DHR6G after intake of a blend of antioxidants-rich fruit and vegetable-based materials. Twelve participants were given 100 mg of a proprietary blend of fruit, vegetable, and herb powders and concentrates commercially marketed under the trade name "Spectra™". Blood samples were collected at 0, 60, 120 and 180 min and were subsequently tested for ROS in serum using DHR6G as a fluorescent probe. By quantifying this fluorescence, we were able to measure ROS concentrations in human blood. This method is both reliable and efficient for evaluating the efficacy of antioxidants against ROS in vivo. Our data indicate that eleven participants responded to the intake of Spectra™ by significant decreases of ROS concentrations.

No MeSH data available.


Related in: MedlinePlus