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Pantoea agglomerans lipopolysaccharide maintains bone density in premenopausal women: a randomized, double-blind, placebo-controlled trial.

Nakata K, Nakata Y, Inagawa H, Nakamoto T, Yoshimura H, Soma G - Food Sci Nutr (2014)

Bottom Line: The results showed that the LPSp group on premenopause maintained their bone density compared with the control group pre- and postmenopause.Moreover, these effects were maintained for 2 months postobservation.LPSp maintains bone volume and density in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutritional Science, Okayama Prefectural University 111 Kuboki, Soja, 719-1197, Japan ; Non-profit Organization, Linking Setouchi Innate Network 2217-16 Hayashi-cho, Takamatsu, Kagawa, 761-0301, Japan.

ABSTRACT
Lipopolysaccharide fromPantoea agglomerans (LPSp) facilitates Ca and P turnover in chicken calvaria and femurs. This study investigated osteoporosis prevention by the oral administration of LPSp in mice and in double-blind clinical tests. Using ovariectomized (OVX) osteoporosis mice model, we investigated the effects of LPSp on the bone density and Ca concentration after ingesting LPSp-containing water for 4 weeks. Oral administration of LPSp tended to suppress the decline in the bone density and the cortical bone thickness in the OVX mice. Moreover, the Ca concentrations were maintained in the OVX-LPSp mice. The effects of LPSp on bone turnover were tested in randomized and double-blind clinical test subjects, who were healthy women aged 40-79 years. The subjects ingested either soy milk without LPSp (control group) or with LPSp (LPSp group) for 3 months. The results showed that the LPSp group on premenopause maintained their bone density compared with the control group pre- and postmenopause. Moreover, these effects were maintained for 2 months postobservation. LPSp maintains bone volume and density in vivo. Thus, a combination of soy milk and LPSp may be useful for osteoporosis prevention.

No MeSH data available.


Related in: MedlinePlus

Change in (A) the bone density, (B) Ca concentration, and (C) cortical bone thickness by ingested LPSp in osteoporosis model mice. The OVX-LPSp mice ingested LPSp (0.05 μg/mL−1 day−1) for 4 weeks. The bone density, Ca concentration, and the cortical bone thickness were analyzed in the femur. Displayed are mean + SE. *P < 0.05.
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fig01: Change in (A) the bone density, (B) Ca concentration, and (C) cortical bone thickness by ingested LPSp in osteoporosis model mice. The OVX-LPSp mice ingested LPSp (0.05 μg/mL−1 day−1) for 4 weeks. The bone density, Ca concentration, and the cortical bone thickness were analyzed in the femur. Displayed are mean + SE. *P < 0.05.

Mentions: We confirmed the effects of ingesting a fermented flour extract that contained LPSp using the OVX mice. Sham-operated or OVX mice had free access to drinking water or the fermented flour extract (LPSp included 0.05 μg/mL) for 4 weeks. We measured their bone density, the Ca concentration, and the cortical bone thickness in the femur. The bone densities of each group were as follows: SHAM-DW mice, 537.0 ± 2.5 mg/cm3; OVX-DW mice, 523.7 ± 6.1 mg/cm3; and OVX-LPSp mice, 541.6 ± 10.1 mg/cm3. The OVX-DW mice had lower bone densities compared with the SHAM-DW mice, while the OVX-LPSp mice had higher densities than the OVX-DW mice. The OVX-LPSp mice were similar to the SHAM-DW (Fig.1A). The Ca concentrations of each group were as follows: SHAM-DW mice, 1.10 ± 0.04 mg/mm; OVX-DW mice, 1.01 ± 0.02 mg/mm; and OVX-LPSp mice, 1.02 ± 0.04 mg/mm. The OVX-DW and the OVX-LPSp mice had lower concentrations than the SHAM-DW mice (Fig.1B). The cortical bone thickness of each group was as follows: SHAM-DW mice, 0.175 ± 0.005 mm; the OVX-DW mice, 0.171 ± 0.002 mm; and the OVX-LPSp mice, 0.181 ± 0.004 mm. The OVX-DW mice had a lower mean bone thickness compared with the SHAM-DW and OVX-LPSp mice. The OVX-LPSp mice were similar to the SHAM-DW mice (Fig.1C). The bone density of OVX-LPSp mice tended to increase (P = 0.15) compared to that of OVX-DW. There were no significant differences between the OVX-DW and the OVX-LPSp mice in Ca concentration, while the cortical bone thickness in the OVX-LPSp mice significantly increased (P < 0.05). Thus, total Ca amount was suggested increasing in the OVX-LPSp mice. These results suggest that LPSp helps to prevent osteoporosis.


Pantoea agglomerans lipopolysaccharide maintains bone density in premenopausal women: a randomized, double-blind, placebo-controlled trial.

Nakata K, Nakata Y, Inagawa H, Nakamoto T, Yoshimura H, Soma G - Food Sci Nutr (2014)

Change in (A) the bone density, (B) Ca concentration, and (C) cortical bone thickness by ingested LPSp in osteoporosis model mice. The OVX-LPSp mice ingested LPSp (0.05 μg/mL−1 day−1) for 4 weeks. The bone density, Ca concentration, and the cortical bone thickness were analyzed in the femur. Displayed are mean + SE. *P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256567&req=5

fig01: Change in (A) the bone density, (B) Ca concentration, and (C) cortical bone thickness by ingested LPSp in osteoporosis model mice. The OVX-LPSp mice ingested LPSp (0.05 μg/mL−1 day−1) for 4 weeks. The bone density, Ca concentration, and the cortical bone thickness were analyzed in the femur. Displayed are mean + SE. *P < 0.05.
Mentions: We confirmed the effects of ingesting a fermented flour extract that contained LPSp using the OVX mice. Sham-operated or OVX mice had free access to drinking water or the fermented flour extract (LPSp included 0.05 μg/mL) for 4 weeks. We measured their bone density, the Ca concentration, and the cortical bone thickness in the femur. The bone densities of each group were as follows: SHAM-DW mice, 537.0 ± 2.5 mg/cm3; OVX-DW mice, 523.7 ± 6.1 mg/cm3; and OVX-LPSp mice, 541.6 ± 10.1 mg/cm3. The OVX-DW mice had lower bone densities compared with the SHAM-DW mice, while the OVX-LPSp mice had higher densities than the OVX-DW mice. The OVX-LPSp mice were similar to the SHAM-DW (Fig.1A). The Ca concentrations of each group were as follows: SHAM-DW mice, 1.10 ± 0.04 mg/mm; OVX-DW mice, 1.01 ± 0.02 mg/mm; and OVX-LPSp mice, 1.02 ± 0.04 mg/mm. The OVX-DW and the OVX-LPSp mice had lower concentrations than the SHAM-DW mice (Fig.1B). The cortical bone thickness of each group was as follows: SHAM-DW mice, 0.175 ± 0.005 mm; the OVX-DW mice, 0.171 ± 0.002 mm; and the OVX-LPSp mice, 0.181 ± 0.004 mm. The OVX-DW mice had a lower mean bone thickness compared with the SHAM-DW and OVX-LPSp mice. The OVX-LPSp mice were similar to the SHAM-DW mice (Fig.1C). The bone density of OVX-LPSp mice tended to increase (P = 0.15) compared to that of OVX-DW. There were no significant differences between the OVX-DW and the OVX-LPSp mice in Ca concentration, while the cortical bone thickness in the OVX-LPSp mice significantly increased (P < 0.05). Thus, total Ca amount was suggested increasing in the OVX-LPSp mice. These results suggest that LPSp helps to prevent osteoporosis.

Bottom Line: The results showed that the LPSp group on premenopause maintained their bone density compared with the control group pre- and postmenopause.Moreover, these effects were maintained for 2 months postobservation.LPSp maintains bone volume and density in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutritional Science, Okayama Prefectural University 111 Kuboki, Soja, 719-1197, Japan ; Non-profit Organization, Linking Setouchi Innate Network 2217-16 Hayashi-cho, Takamatsu, Kagawa, 761-0301, Japan.

ABSTRACT
Lipopolysaccharide fromPantoea agglomerans (LPSp) facilitates Ca and P turnover in chicken calvaria and femurs. This study investigated osteoporosis prevention by the oral administration of LPSp in mice and in double-blind clinical tests. Using ovariectomized (OVX) osteoporosis mice model, we investigated the effects of LPSp on the bone density and Ca concentration after ingesting LPSp-containing water for 4 weeks. Oral administration of LPSp tended to suppress the decline in the bone density and the cortical bone thickness in the OVX mice. Moreover, the Ca concentrations were maintained in the OVX-LPSp mice. The effects of LPSp on bone turnover were tested in randomized and double-blind clinical test subjects, who were healthy women aged 40-79 years. The subjects ingested either soy milk without LPSp (control group) or with LPSp (LPSp group) for 3 months. The results showed that the LPSp group on premenopause maintained their bone density compared with the control group pre- and postmenopause. Moreover, these effects were maintained for 2 months postobservation. LPSp maintains bone volume and density in vivo. Thus, a combination of soy milk and LPSp may be useful for osteoporosis prevention.

No MeSH data available.


Related in: MedlinePlus