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Uptake and intracytoplasmic storage of pigmented particles by human CD34+ stromal cells/telocytes: endocytic property of telocytes.

Díaz-Flores L, Gutiérrez R, García MP, Sáez FJ, Aparicio F, Díaz-Flores L, Madrid JF - J. Cell. Mol. Med. (2014)

Bottom Line: Our results demonstrate that CD34+ TCs have endocytic properties (phagocytic-like TCs: phTCs), with the capacity to uptake and store India ink particles. phTCs conserve the characteristics of TCs (long, thin, bipolar or multipolar, moniliform cytoplasmic processes/telopodes, with linear distribution of the pigment) and maintain their typical distribution.Our ultrastructural observation of melanin-storing stromal cells with characteristics of TCs (telopodes with dichotomous branching pattern) favours this possibility.In conclusion, intestinal TCs have a phagocytic-like property, a function that may be generalized to TCs in other locations.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Pathology, Histology and Radiology, Faculty of Medicine, University of La Laguna, Tenerife, Spain.

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(A and C) CD34+ phTCs with pigment (arrows) near a nest of naevus cells (A, nc) and around a sweat gland (C, sg) in pigmented melanocytic nevi. (B) Stromal cells with pigment (arrows) near naevus cells (nc) in an haematoxylin and eosin stained section. (D and E) phTCs with ultrastructural characteristics of TCs show endocytozed melanosomes (arrows). Note that naevus cells present a basal lamina, whereas the TCs do not. In the insert of D, CD34+ phTCs with intracytoplasmic pigment. m, Macrophages.
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fig06: (A and C) CD34+ phTCs with pigment (arrows) near a nest of naevus cells (A, nc) and around a sweat gland (C, sg) in pigmented melanocytic nevi. (B) Stromal cells with pigment (arrows) near naevus cells (nc) in an haematoxylin and eosin stained section. (D and E) phTCs with ultrastructural characteristics of TCs show endocytozed melanosomes (arrows). Note that naevus cells present a basal lamina, whereas the TCs do not. In the insert of D, CD34+ phTCs with intracytoplasmic pigment. m, Macrophages.

Mentions: In compound melanocytic nevi, pigmented intracytoplasmic particles were observed in CD34+ stromal cells arranged near nests of naevus cells (Fig. 6A) and skin annexes (Fig. 6C). In tissue sections stained with haematoxylin and eosin, stromal cells with intracellular pigment were also observed near naevus cells (Fig. 6B). Under electron microscopy, the particles were endocytozed melanosomes and the cells showed ultrastructural characteristics of TCs (long, thin telopodes with podomeres and podoms, dichotomous branching pattern and absence of basal membrane; Fig. 6D and E).


Uptake and intracytoplasmic storage of pigmented particles by human CD34+ stromal cells/telocytes: endocytic property of telocytes.

Díaz-Flores L, Gutiérrez R, García MP, Sáez FJ, Aparicio F, Díaz-Flores L, Madrid JF - J. Cell. Mol. Med. (2014)

(A and C) CD34+ phTCs with pigment (arrows) near a nest of naevus cells (A, nc) and around a sweat gland (C, sg) in pigmented melanocytic nevi. (B) Stromal cells with pigment (arrows) near naevus cells (nc) in an haematoxylin and eosin stained section. (D and E) phTCs with ultrastructural characteristics of TCs show endocytozed melanosomes (arrows). Note that naevus cells present a basal lamina, whereas the TCs do not. In the insert of D, CD34+ phTCs with intracytoplasmic pigment. m, Macrophages.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256559&req=5

fig06: (A and C) CD34+ phTCs with pigment (arrows) near a nest of naevus cells (A, nc) and around a sweat gland (C, sg) in pigmented melanocytic nevi. (B) Stromal cells with pigment (arrows) near naevus cells (nc) in an haematoxylin and eosin stained section. (D and E) phTCs with ultrastructural characteristics of TCs show endocytozed melanosomes (arrows). Note that naevus cells present a basal lamina, whereas the TCs do not. In the insert of D, CD34+ phTCs with intracytoplasmic pigment. m, Macrophages.
Mentions: In compound melanocytic nevi, pigmented intracytoplasmic particles were observed in CD34+ stromal cells arranged near nests of naevus cells (Fig. 6A) and skin annexes (Fig. 6C). In tissue sections stained with haematoxylin and eosin, stromal cells with intracellular pigment were also observed near naevus cells (Fig. 6B). Under electron microscopy, the particles were endocytozed melanosomes and the cells showed ultrastructural characteristics of TCs (long, thin telopodes with podomeres and podoms, dichotomous branching pattern and absence of basal membrane; Fig. 6D and E).

Bottom Line: Our results demonstrate that CD34+ TCs have endocytic properties (phagocytic-like TCs: phTCs), with the capacity to uptake and store India ink particles. phTCs conserve the characteristics of TCs (long, thin, bipolar or multipolar, moniliform cytoplasmic processes/telopodes, with linear distribution of the pigment) and maintain their typical distribution.Our ultrastructural observation of melanin-storing stromal cells with characteristics of TCs (telopodes with dichotomous branching pattern) favours this possibility.In conclusion, intestinal TCs have a phagocytic-like property, a function that may be generalized to TCs in other locations.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Pathology, Histology and Radiology, Faculty of Medicine, University of La Laguna, Tenerife, Spain.

Show MeSH
Related in: MedlinePlus