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Uptake and intracytoplasmic storage of pigmented particles by human CD34+ stromal cells/telocytes: endocytic property of telocytes.

Díaz-Flores L, Gutiérrez R, García MP, Sáez FJ, Aparicio F, Díaz-Flores L, Madrid JF - J. Cell. Mol. Med. (2014)

Bottom Line: Our results demonstrate that CD34+ TCs have endocytic properties (phagocytic-like TCs: phTCs), with the capacity to uptake and store India ink particles. phTCs conserve the characteristics of TCs (long, thin, bipolar or multipolar, moniliform cytoplasmic processes/telopodes, with linear distribution of the pigment) and maintain their typical distribution.Our ultrastructural observation of melanin-storing stromal cells with characteristics of TCs (telopodes with dichotomous branching pattern) favours this possibility.In conclusion, intestinal TCs have a phagocytic-like property, a function that may be generalized to TCs in other locations.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Pathology, Histology and Radiology, Faculty of Medicine, University of La Laguna, Tenerife, Spain.

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Enteric wall tattooed with India ink. (A) CD34+ phTCs around the basal portion of a gland (g) and lining the mucosa surface of muscularis mucosae (mm). (B and C) CD34+ phTCs (arrows) arranged around myenteric plexus ganglia. (D) A CD34+ phTC (arrow) between adipocytes (ad). (E) An occasional CD117 (c-kit) stained interstitial cell of Cajal (ICC) with endocytozed India ink particles (arrows).
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fig03: Enteric wall tattooed with India ink. (A) CD34+ phTCs around the basal portion of a gland (g) and lining the mucosa surface of muscularis mucosae (mm). (B and C) CD34+ phTCs (arrows) arranged around myenteric plexus ganglia. (D) A CD34+ phTC (arrow) between adipocytes (ad). (E) An occasional CD117 (c-kit) stained interstitial cell of Cajal (ICC) with endocytozed India ink particles (arrows).

Mentions: CD34+ phTCs were observed in some deep regions of the mucosa and in the submucosa, muscular propria and serosa. In the rest of the mucosa, CD34+ TCs or CD34+ phTCs were not detected. In all pigmented layers of the enteric wall, phTCs surrounded vessels of different calibre (encircling the media layer; Fig. 2) and nerves. In the deep region of the lamina propria, scattered phTCs were located around the basal portion of some intestinal glands (Fig. 3A). In the muscularis mucosae, abundant phTCs were observed in its mucosa (Fig. 3A) and submucosa surfaces, and around groups of smooth muscle cells (SMCs). In the submucosa, phTCs formed networks between collagen and elastic fibres and two almost continuous layers bordering on the muscularis mucosae and the muscular propria covering the submucosal border of the circular muscle layer. The submucous plexus ganglia were also encircled by phTCs. In both layers of muscular propria, phTCs were arranged around fascicles and bundles of SMCs (in septal borders) and between SMCs (Fig. 1D and E). In Auerbach's plexuses, phTCs surrounded ganglia (covering cells; Fig. 3B and C) and formed networks in nerve strands and between the ganglia (Fig. 1F). CD34+ phTCs were also present in peri-intestinal adipose tissue when the injected pigment spread towards it. In this location, phTCs formed a delicate network that surrounded adipose lobes and lobules. The cytoplasmic bodies and telopodes of phTCs were also closely associated with adipocytes (Fig. 3D).


Uptake and intracytoplasmic storage of pigmented particles by human CD34+ stromal cells/telocytes: endocytic property of telocytes.

Díaz-Flores L, Gutiérrez R, García MP, Sáez FJ, Aparicio F, Díaz-Flores L, Madrid JF - J. Cell. Mol. Med. (2014)

Enteric wall tattooed with India ink. (A) CD34+ phTCs around the basal portion of a gland (g) and lining the mucosa surface of muscularis mucosae (mm). (B and C) CD34+ phTCs (arrows) arranged around myenteric plexus ganglia. (D) A CD34+ phTC (arrow) between adipocytes (ad). (E) An occasional CD117 (c-kit) stained interstitial cell of Cajal (ICC) with endocytozed India ink particles (arrows).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256559&req=5

fig03: Enteric wall tattooed with India ink. (A) CD34+ phTCs around the basal portion of a gland (g) and lining the mucosa surface of muscularis mucosae (mm). (B and C) CD34+ phTCs (arrows) arranged around myenteric plexus ganglia. (D) A CD34+ phTC (arrow) between adipocytes (ad). (E) An occasional CD117 (c-kit) stained interstitial cell of Cajal (ICC) with endocytozed India ink particles (arrows).
Mentions: CD34+ phTCs were observed in some deep regions of the mucosa and in the submucosa, muscular propria and serosa. In the rest of the mucosa, CD34+ TCs or CD34+ phTCs were not detected. In all pigmented layers of the enteric wall, phTCs surrounded vessels of different calibre (encircling the media layer; Fig. 2) and nerves. In the deep region of the lamina propria, scattered phTCs were located around the basal portion of some intestinal glands (Fig. 3A). In the muscularis mucosae, abundant phTCs were observed in its mucosa (Fig. 3A) and submucosa surfaces, and around groups of smooth muscle cells (SMCs). In the submucosa, phTCs formed networks between collagen and elastic fibres and two almost continuous layers bordering on the muscularis mucosae and the muscular propria covering the submucosal border of the circular muscle layer. The submucous plexus ganglia were also encircled by phTCs. In both layers of muscular propria, phTCs were arranged around fascicles and bundles of SMCs (in septal borders) and between SMCs (Fig. 1D and E). In Auerbach's plexuses, phTCs surrounded ganglia (covering cells; Fig. 3B and C) and formed networks in nerve strands and between the ganglia (Fig. 1F). CD34+ phTCs were also present in peri-intestinal adipose tissue when the injected pigment spread towards it. In this location, phTCs formed a delicate network that surrounded adipose lobes and lobules. The cytoplasmic bodies and telopodes of phTCs were also closely associated with adipocytes (Fig. 3D).

Bottom Line: Our results demonstrate that CD34+ TCs have endocytic properties (phagocytic-like TCs: phTCs), with the capacity to uptake and store India ink particles. phTCs conserve the characteristics of TCs (long, thin, bipolar or multipolar, moniliform cytoplasmic processes/telopodes, with linear distribution of the pigment) and maintain their typical distribution.Our ultrastructural observation of melanin-storing stromal cells with characteristics of TCs (telopodes with dichotomous branching pattern) favours this possibility.In conclusion, intestinal TCs have a phagocytic-like property, a function that may be generalized to TCs in other locations.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Pathology, Histology and Radiology, Faculty of Medicine, University of La Laguna, Tenerife, Spain.

Show MeSH
Related in: MedlinePlus