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Is serum Interleukin-17 associated with early atherosclerosis in obese patients?

Tarantino G, Costantini S, Finelli C, Capone F, Guerriero E, La Sala N, Gioia S, Castello G - J Transl Med (2014)

Bottom Line: At multiple regression,IMT was predicted, other than by age, by the amount of the visceral adiposity, expressed as visceral adipose tissue at ultrasonography, and by serum eotaxin.In conclusion, a strong relationship was found between the IL-17-related chemokine eotaxin and IMT.The association found between the amount of visceral fat and circulating levels of eotaxin on the one hand, and IMT on the other, could reinforce the hypothesis that IL-17, released by the visceral adipose tissue, induces eotaxin secretion via the smooth muscle cells present in the atheromatosus vessels.

View Article: PubMed Central - PubMed

ABSTRACT
Atherosclerosis is a chronic inflammatory process of the vessel walls, and CD4+ T-cells are peculiar to both human and murine atherosclerotic lesions. There is a recent line of research favoring hypothetic allergic mechanisms in the genesis of atherosclerosis and, consequently, coronary artery disease (CAD), among which Interleukin (IL)-17 appears to be a key cytokine regulating local tissue inflammation. The objective was to add a piece of information on the role of IL-17 in the genesis of atherosclerosis. Eighty obese patients with normal liver enzyme levels but presenting with ultrasonographic evidence of NAFLD formed the population of this cross-sectional study. Anthropometric measures, data on excess adiposity, metabolic profile, serum concentrations of IL-17, eotaxin-3, IL-8, and CCL4/MIP1β, C-reactive protein, fibrinogen, ferritin, TNF-α, as well carotid intima-media thickness (IMT), a marker of atherosclerosis, and the main risk factors for CAD, such as blood pressure and smoking status, but also less determinant ones such as degree of NAFLD severity, Intramuscular Triglyceride storage and Resting Metabolic Rate were evaluated. Serum concentrations of Il-17 were detected as related to those of inflammatory cytokines, IL-6, IFN-γ and TNF-α. Furthermore, circulating levels of IL-17 were linked to those mirroring allergic process, IL-8, CCL4/MIP1β and eotaxin. Early atherosclerosis, evidenced as increased IMT, was not associated with circulating IL-17 levels. At multiple regression,IMT was predicted, other than by age, by the amount of the visceral adiposity, expressed as visceral adipose tissue at ultrasonography, and by serum eotaxin. In conclusion, a strong relationship was found between the IL-17-related chemokine eotaxin and IMT. The association found between the amount of visceral fat and circulating levels of eotaxin on the one hand, and IMT on the other, could reinforce the hypothesis that IL-17, released by the visceral adipose tissue, induces eotaxin secretion via the smooth muscle cells present in the atheromatosus vessels.

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Related in: MedlinePlus

Graphics of the main predictions between IL-17 and other immune parameters. At the centre the regression line is evidenced; 95% confidence interval as dot line and 95% prediction interval as continuous line, Chemokine (C-C motif) ligand 4 CCL4/MIP1β, Interferon-Gamma IFN-γ, Interleukin-17 IL-17, Interleukin-8 IL-8.
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Fig1: Graphics of the main predictions between IL-17 and other immune parameters. At the centre the regression line is evidenced; 95% confidence interval as dot line and 95% prediction interval as continuous line, Chemokine (C-C motif) ligand 4 CCL4/MIP1β, Interferon-Gamma IFN-γ, Interleukin-17 IL-17, Interleukin-8 IL-8.

Mentions: The main findings were firstly that, at univariate analysis, serum concentrations of Il-17 were not associated with IMT. Secondly, circulating concentrations of Il-17 were related to the levels of specific inflammatory/immune regulators, such as TNF α, IL-6, IL-8, IFN γ, CCL4/MIP1β, Eotaxin, but not of other inflammatory parameters such as CRP, fibrinogen and ferritin (Table 2). Noteworthy were the strict relationships between serum IL-17 and the parameters strongly up-regulated in allergic/hyperergic reactions, such as eotaxin, IL-8, and CCL4/MIP1β (Table 2, Figure 1). Similarly, IL-8 and eotaxin were the best combination of predictors of their serum levels at multivariate analysis. Of interest, at univariate analysis, IMT was well predicted by the fat deposition in the abdominal, liver and muscle districts, specifically, the thickness of visceral adiposity expressed as VAT, the grade of HS at US, the deposition of TG in muscle, i.e., ImTG, by an IL-17-induced chemokine, i.e., circulating eotaxin, and a marker of chronic inflammation, i.e., serum ferritin, but not by the smoking status (Table 3). Insulin resistance, evaluated as HOMA, and anthropometric features such as BMI, WC and WHR were not associated with serum IL-17 levels, nor were US parameters of ectopic fat storage, such as HS, SAT, VAT and ImTG, related to the same cytokine (Table 3).Table 2


Is serum Interleukin-17 associated with early atherosclerosis in obese patients?

Tarantino G, Costantini S, Finelli C, Capone F, Guerriero E, La Sala N, Gioia S, Castello G - J Transl Med (2014)

Graphics of the main predictions between IL-17 and other immune parameters. At the centre the regression line is evidenced; 95% confidence interval as dot line and 95% prediction interval as continuous line, Chemokine (C-C motif) ligand 4 CCL4/MIP1β, Interferon-Gamma IFN-γ, Interleukin-17 IL-17, Interleukin-8 IL-8.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4256548&req=5

Fig1: Graphics of the main predictions between IL-17 and other immune parameters. At the centre the regression line is evidenced; 95% confidence interval as dot line and 95% prediction interval as continuous line, Chemokine (C-C motif) ligand 4 CCL4/MIP1β, Interferon-Gamma IFN-γ, Interleukin-17 IL-17, Interleukin-8 IL-8.
Mentions: The main findings were firstly that, at univariate analysis, serum concentrations of Il-17 were not associated with IMT. Secondly, circulating concentrations of Il-17 were related to the levels of specific inflammatory/immune regulators, such as TNF α, IL-6, IL-8, IFN γ, CCL4/MIP1β, Eotaxin, but not of other inflammatory parameters such as CRP, fibrinogen and ferritin (Table 2). Noteworthy were the strict relationships between serum IL-17 and the parameters strongly up-regulated in allergic/hyperergic reactions, such as eotaxin, IL-8, and CCL4/MIP1β (Table 2, Figure 1). Similarly, IL-8 and eotaxin were the best combination of predictors of their serum levels at multivariate analysis. Of interest, at univariate analysis, IMT was well predicted by the fat deposition in the abdominal, liver and muscle districts, specifically, the thickness of visceral adiposity expressed as VAT, the grade of HS at US, the deposition of TG in muscle, i.e., ImTG, by an IL-17-induced chemokine, i.e., circulating eotaxin, and a marker of chronic inflammation, i.e., serum ferritin, but not by the smoking status (Table 3). Insulin resistance, evaluated as HOMA, and anthropometric features such as BMI, WC and WHR were not associated with serum IL-17 levels, nor were US parameters of ectopic fat storage, such as HS, SAT, VAT and ImTG, related to the same cytokine (Table 3).Table 2

Bottom Line: At multiple regression,IMT was predicted, other than by age, by the amount of the visceral adiposity, expressed as visceral adipose tissue at ultrasonography, and by serum eotaxin.In conclusion, a strong relationship was found between the IL-17-related chemokine eotaxin and IMT.The association found between the amount of visceral fat and circulating levels of eotaxin on the one hand, and IMT on the other, could reinforce the hypothesis that IL-17, released by the visceral adipose tissue, induces eotaxin secretion via the smooth muscle cells present in the atheromatosus vessels.

View Article: PubMed Central - PubMed

ABSTRACT
Atherosclerosis is a chronic inflammatory process of the vessel walls, and CD4+ T-cells are peculiar to both human and murine atherosclerotic lesions. There is a recent line of research favoring hypothetic allergic mechanisms in the genesis of atherosclerosis and, consequently, coronary artery disease (CAD), among which Interleukin (IL)-17 appears to be a key cytokine regulating local tissue inflammation. The objective was to add a piece of information on the role of IL-17 in the genesis of atherosclerosis. Eighty obese patients with normal liver enzyme levels but presenting with ultrasonographic evidence of NAFLD formed the population of this cross-sectional study. Anthropometric measures, data on excess adiposity, metabolic profile, serum concentrations of IL-17, eotaxin-3, IL-8, and CCL4/MIP1β, C-reactive protein, fibrinogen, ferritin, TNF-α, as well carotid intima-media thickness (IMT), a marker of atherosclerosis, and the main risk factors for CAD, such as blood pressure and smoking status, but also less determinant ones such as degree of NAFLD severity, Intramuscular Triglyceride storage and Resting Metabolic Rate were evaluated. Serum concentrations of Il-17 were detected as related to those of inflammatory cytokines, IL-6, IFN-γ and TNF-α. Furthermore, circulating levels of IL-17 were linked to those mirroring allergic process, IL-8, CCL4/MIP1β and eotaxin. Early atherosclerosis, evidenced as increased IMT, was not associated with circulating IL-17 levels. At multiple regression,IMT was predicted, other than by age, by the amount of the visceral adiposity, expressed as visceral adipose tissue at ultrasonography, and by serum eotaxin. In conclusion, a strong relationship was found between the IL-17-related chemokine eotaxin and IMT. The association found between the amount of visceral fat and circulating levels of eotaxin on the one hand, and IMT on the other, could reinforce the hypothesis that IL-17, released by the visceral adipose tissue, induces eotaxin secretion via the smooth muscle cells present in the atheromatosus vessels.

Show MeSH
Related in: MedlinePlus