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An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.

Umetsu D, Dunst S, Dahmann C - PLoS ONE (2014)

Bottom Line: Out of screening 3114 transgenic RNA interference lines targeting a total of 2863 genes, we identified a single novel candidate that interfered with the formation of a straight anteroposterior compartment boundary.Interestingly, the targeted gene encodes for the Eph receptor tyrosine kinase, an evolutionarily conserved family of signal transducers that has previously been shown to be important for maintaining straight compartment boundaries in vertebrate embryos.Our results identify a hitherto unknown role of the Eph receptor tyrosine kinase in Drosophila and suggest that Eph receptors have important functions in shaping compartment boundaries in both vertebrate and insect development.

View Article: PubMed Central - PubMed

Affiliation: Institute of Genetics, Technische Universit├Ąt Dresden, Dresden, Germany.

ABSTRACT
The formation of straight compartment boundaries separating groups of cells with distinct fates and functions is an evolutionarily conserved strategy during animal development. The physical mechanisms that shape compartment boundaries have recently been further elucidated, however, the molecular mechanisms that underlie compartment boundary formation and maintenance remain poorly understood. Here, we report on the outcome of an RNA interference screen aimed at identifying novel genes involved in maintaining the straight shape of the anteroposterior compartment boundary in Drosophila wing imaginal discs. Out of screening 3114 transgenic RNA interference lines targeting a total of 2863 genes, we identified a single novel candidate that interfered with the formation of a straight anteroposterior compartment boundary. Interestingly, the targeted gene encodes for the Eph receptor tyrosine kinase, an evolutionarily conserved family of signal transducers that has previously been shown to be important for maintaining straight compartment boundaries in vertebrate embryos. Our results identify a hitherto unknown role of the Eph receptor tyrosine kinase in Drosophila and suggest that Eph receptors have important functions in shaping compartment boundaries in both vertebrate and insect development.

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Summary of RNA interference screen.A. Number of RNAi lines screened categorized for the class of targeted gene products. B. Number of the analyzed RNAi lines and fractions of the phenotypic categories as a percentage.
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pone-0114340-g003: Summary of RNA interference screen.A. Number of RNAi lines screened categorized for the class of targeted gene products. B. Number of the analyzed RNAi lines and fractions of the phenotypic categories as a percentage.

Mentions: We next used the assay to screen 3114 selected RNAi lines targeting a total of 2863 genes [37]. We mainly sought to analyze RNAi lines that targeted genes encoding transmembrane proteins. Transmembrane proteins can mediate cell-cell communication, cell-cell recognition or cell adhesion that might be important for maintaining the shape of the AP boundary. 3181 genes encoding transmembrane proteins were identified in Drosophila based on sequence analysis (Krystyna Keleman, personal communication). We analyzed RNAi lines targeting 2204 of these genes encoding for transmembrane proteins. In addition, we analyzed RNAi lines targeting genes involved in cytoskeletal (114 genes, 4.0%) or transcriptional (127 genes, 4.4%) regulation. A classification of the proteins encoded by the targeted genes based on protein function [38] is detailed in Fig. 3A. The persons performing and analyzing the RNA interference screen had no knowledge of the identity of the targeted gene products.


An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.

Umetsu D, Dunst S, Dahmann C - PLoS ONE (2014)

Summary of RNA interference screen.A. Number of RNAi lines screened categorized for the class of targeted gene products. B. Number of the analyzed RNAi lines and fractions of the phenotypic categories as a percentage.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256218&req=5

pone-0114340-g003: Summary of RNA interference screen.A. Number of RNAi lines screened categorized for the class of targeted gene products. B. Number of the analyzed RNAi lines and fractions of the phenotypic categories as a percentage.
Mentions: We next used the assay to screen 3114 selected RNAi lines targeting a total of 2863 genes [37]. We mainly sought to analyze RNAi lines that targeted genes encoding transmembrane proteins. Transmembrane proteins can mediate cell-cell communication, cell-cell recognition or cell adhesion that might be important for maintaining the shape of the AP boundary. 3181 genes encoding transmembrane proteins were identified in Drosophila based on sequence analysis (Krystyna Keleman, personal communication). We analyzed RNAi lines targeting 2204 of these genes encoding for transmembrane proteins. In addition, we analyzed RNAi lines targeting genes involved in cytoskeletal (114 genes, 4.0%) or transcriptional (127 genes, 4.4%) regulation. A classification of the proteins encoded by the targeted genes based on protein function [38] is detailed in Fig. 3A. The persons performing and analyzing the RNA interference screen had no knowledge of the identity of the targeted gene products.

Bottom Line: Out of screening 3114 transgenic RNA interference lines targeting a total of 2863 genes, we identified a single novel candidate that interfered with the formation of a straight anteroposterior compartment boundary.Interestingly, the targeted gene encodes for the Eph receptor tyrosine kinase, an evolutionarily conserved family of signal transducers that has previously been shown to be important for maintaining straight compartment boundaries in vertebrate embryos.Our results identify a hitherto unknown role of the Eph receptor tyrosine kinase in Drosophila and suggest that Eph receptors have important functions in shaping compartment boundaries in both vertebrate and insect development.

View Article: PubMed Central - PubMed

Affiliation: Institute of Genetics, Technische Universit├Ąt Dresden, Dresden, Germany.

ABSTRACT
The formation of straight compartment boundaries separating groups of cells with distinct fates and functions is an evolutionarily conserved strategy during animal development. The physical mechanisms that shape compartment boundaries have recently been further elucidated, however, the molecular mechanisms that underlie compartment boundary formation and maintenance remain poorly understood. Here, we report on the outcome of an RNA interference screen aimed at identifying novel genes involved in maintaining the straight shape of the anteroposterior compartment boundary in Drosophila wing imaginal discs. Out of screening 3114 transgenic RNA interference lines targeting a total of 2863 genes, we identified a single novel candidate that interfered with the formation of a straight anteroposterior compartment boundary. Interestingly, the targeted gene encodes for the Eph receptor tyrosine kinase, an evolutionarily conserved family of signal transducers that has previously been shown to be important for maintaining straight compartment boundaries in vertebrate embryos. Our results identify a hitherto unknown role of the Eph receptor tyrosine kinase in Drosophila and suggest that Eph receptors have important functions in shaping compartment boundaries in both vertebrate and insect development.

Show MeSH