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Electronically monitored labial dabbing and stylet 'probing' behaviors of brown marmorated stink bug, Halyomorpha halys, in simulated environments.

Wiman NG, Walton VM, Shearer PW, Rondon SI - PLoS ONE (2014)

Bottom Line: We found that temperature had a significant impact on H. halys 'probing' behavior and may influence periodicity of activity.Our data suggest that the minimal temperature at which 'probing' of H. halys occurs is between 3.5 and 6.1 °C (95% CI), and that 'probing' does not occur at temperatures above 26.5 to 29.6 °C (95% CI).We estimated that the optimal temperature for 'probing' is between 16 and 17 °C.

View Article: PubMed Central - PubMed

Affiliation: Department of Horticulture, Oregon State University, Corvallis, Oregon, United States of America.

ABSTRACT
Brown marmorated stink bug, Halyomorpha halys (Stål), (Hemiptera: Pentatomidae) is an invasive polyphagous agricultural and urban nuisance pest of Asian origin that is becoming widespread in North America and Europe. Despite the economic importance of pentatomid pests worldwide, their feeding behavior is poorly understood. Electronically monitored insect feeding (EMIF) technology is a useful tool in studies of feeding behavior of Hemiptera. Here we examined H. halys feeding behavior using an EMIF system designed for high throughput studies in environmental chambers. Our objectives were to quantify feeding activity by monitoring proboscis contacts with green beans, including labial dabbing and stylet penetration of the beans, which we collectively define as 'probes'. We examined frequency and duration of 'probes' in field-collected H. halys over 48 hours and we determined how environmental conditions could affect diel and seasonal periodicity of 'probing' activity. We found differences in 'probing' activity between months when the assays were conducted. These differences in activity may have reflected different environmental conditions, and they also coincide with what is known about the phenology of H. halys. While a substantial number of 'probes' occurred during scotophase, including some of the longest mean 'probe' durations, activity was either lower or similar to 'probing' activity levels during photophase on average. We found that temperature had a significant impact on H. halys 'probing' behavior and may influence periodicity of activity. Our data suggest that the minimal temperature at which 'probing' of H. halys occurs is between 3.5 and 6.1 °C (95% CI), and that 'probing' does not occur at temperatures above 26.5 to 29.6 °C (95% CI). We estimated that the optimal temperature for 'probing' is between 16 and 17 °C.

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Diel ‘probing’ activity comparison (±95% CI); proportion of H. halys ‘probing’ (a–b); ‘probes’ per insect per day (c–d), mean ‘probe’ duration (e–f).
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pone-0113514-g009: Diel ‘probing’ activity comparison (±95% CI); proportion of H. halys ‘probing’ (a–b); ‘probes’ per insect per day (c–d), mean ‘probe’ duration (e–f).

Mentions: In the warm chamber, a significantly higher proportion of the insects ‘probed’ during photophase (0.95) compared to scotophase (0.43; binomial test; P<0.001; Figure 9a). Similarly, in the cold chamber, the peak proportion of ‘probing’ H. halys (0.36) was recorded within the hours of 18∶00 and 19∶00 (Figure 8b). The observed maximum proportion of H. halys ‘probing’ was 0.45, which occurred between the hours of 16∶00 and 17∶00. Nonetheless, the lowest predicted observed proportion of ‘probing’ insects (0.14) occurred during scotophase between the hours of 00∶00 and 01∶00. The proportion of insects ‘probing’ during photophase (0.89) was again significantly higher than the proportion feeding (0.49) during scotophase (binomial test; P<0.001; Figure 9b).


Electronically monitored labial dabbing and stylet 'probing' behaviors of brown marmorated stink bug, Halyomorpha halys, in simulated environments.

Wiman NG, Walton VM, Shearer PW, Rondon SI - PLoS ONE (2014)

Diel ‘probing’ activity comparison (±95% CI); proportion of H. halys ‘probing’ (a–b); ‘probes’ per insect per day (c–d), mean ‘probe’ duration (e–f).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256200&req=5

pone-0113514-g009: Diel ‘probing’ activity comparison (±95% CI); proportion of H. halys ‘probing’ (a–b); ‘probes’ per insect per day (c–d), mean ‘probe’ duration (e–f).
Mentions: In the warm chamber, a significantly higher proportion of the insects ‘probed’ during photophase (0.95) compared to scotophase (0.43; binomial test; P<0.001; Figure 9a). Similarly, in the cold chamber, the peak proportion of ‘probing’ H. halys (0.36) was recorded within the hours of 18∶00 and 19∶00 (Figure 8b). The observed maximum proportion of H. halys ‘probing’ was 0.45, which occurred between the hours of 16∶00 and 17∶00. Nonetheless, the lowest predicted observed proportion of ‘probing’ insects (0.14) occurred during scotophase between the hours of 00∶00 and 01∶00. The proportion of insects ‘probing’ during photophase (0.89) was again significantly higher than the proportion feeding (0.49) during scotophase (binomial test; P<0.001; Figure 9b).

Bottom Line: We found that temperature had a significant impact on H. halys 'probing' behavior and may influence periodicity of activity.Our data suggest that the minimal temperature at which 'probing' of H. halys occurs is between 3.5 and 6.1 °C (95% CI), and that 'probing' does not occur at temperatures above 26.5 to 29.6 °C (95% CI).We estimated that the optimal temperature for 'probing' is between 16 and 17 °C.

View Article: PubMed Central - PubMed

Affiliation: Department of Horticulture, Oregon State University, Corvallis, Oregon, United States of America.

ABSTRACT
Brown marmorated stink bug, Halyomorpha halys (Stål), (Hemiptera: Pentatomidae) is an invasive polyphagous agricultural and urban nuisance pest of Asian origin that is becoming widespread in North America and Europe. Despite the economic importance of pentatomid pests worldwide, their feeding behavior is poorly understood. Electronically monitored insect feeding (EMIF) technology is a useful tool in studies of feeding behavior of Hemiptera. Here we examined H. halys feeding behavior using an EMIF system designed for high throughput studies in environmental chambers. Our objectives were to quantify feeding activity by monitoring proboscis contacts with green beans, including labial dabbing and stylet penetration of the beans, which we collectively define as 'probes'. We examined frequency and duration of 'probes' in field-collected H. halys over 48 hours and we determined how environmental conditions could affect diel and seasonal periodicity of 'probing' activity. We found differences in 'probing' activity between months when the assays were conducted. These differences in activity may have reflected different environmental conditions, and they also coincide with what is known about the phenology of H. halys. While a substantial number of 'probes' occurred during scotophase, including some of the longest mean 'probe' durations, activity was either lower or similar to 'probing' activity levels during photophase on average. We found that temperature had a significant impact on H. halys 'probing' behavior and may influence periodicity of activity. Our data suggest that the minimal temperature at which 'probing' of H. halys occurs is between 3.5 and 6.1 °C (95% CI), and that 'probing' does not occur at temperatures above 26.5 to 29.6 °C (95% CI). We estimated that the optimal temperature for 'probing' is between 16 and 17 °C.

Show MeSH
Related in: MedlinePlus