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Electronically monitored labial dabbing and stylet 'probing' behaviors of brown marmorated stink bug, Halyomorpha halys, in simulated environments.

Wiman NG, Walton VM, Shearer PW, Rondon SI - PLoS ONE (2014)

Bottom Line: We found that temperature had a significant impact on H. halys 'probing' behavior and may influence periodicity of activity.Our data suggest that the minimal temperature at which 'probing' of H. halys occurs is between 3.5 and 6.1 °C (95% CI), and that 'probing' does not occur at temperatures above 26.5 to 29.6 °C (95% CI).We estimated that the optimal temperature for 'probing' is between 16 and 17 °C.

View Article: PubMed Central - PubMed

Affiliation: Department of Horticulture, Oregon State University, Corvallis, Oregon, United States of America.

ABSTRACT
Brown marmorated stink bug, Halyomorpha halys (Stål), (Hemiptera: Pentatomidae) is an invasive polyphagous agricultural and urban nuisance pest of Asian origin that is becoming widespread in North America and Europe. Despite the economic importance of pentatomid pests worldwide, their feeding behavior is poorly understood. Electronically monitored insect feeding (EMIF) technology is a useful tool in studies of feeding behavior of Hemiptera. Here we examined H. halys feeding behavior using an EMIF system designed for high throughput studies in environmental chambers. Our objectives were to quantify feeding activity by monitoring proboscis contacts with green beans, including labial dabbing and stylet penetration of the beans, which we collectively define as 'probes'. We examined frequency and duration of 'probes' in field-collected H. halys over 48 hours and we determined how environmental conditions could affect diel and seasonal periodicity of 'probing' activity. We found differences in 'probing' activity between months when the assays were conducted. These differences in activity may have reflected different environmental conditions, and they also coincide with what is known about the phenology of H. halys. While a substantial number of 'probes' occurred during scotophase, including some of the longest mean 'probe' durations, activity was either lower or similar to 'probing' activity levels during photophase on average. We found that temperature had a significant impact on H. halys 'probing' behavior and may influence periodicity of activity. Our data suggest that the minimal temperature at which 'probing' of H. halys occurs is between 3.5 and 6.1 °C (95% CI), and that 'probing' does not occur at temperatures above 26.5 to 29.6 °C (95% CI). We estimated that the optimal temperature for 'probing' is between 16 and 17 °C.

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‘Probing’ duration and total ‘probing’ time for H. halys in each of warm and cool environmental chambers.
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pone-0113514-g007: ‘Probing’ duration and total ‘probing’ time for H. halys in each of warm and cool environmental chambers.

Mentions: The duration of ‘probes’ depended on the month in which the assay was conducted in both the warm (F = 8.11; d.f. = 5,84; P<0.001; Figure 7a), and the cool environmental chambers (F = 2.38; d.f. = 5,101; P = 0.043; Figure 7b). Some of the longest mean ‘probe’ durations occurred in chambers programmed to represent early season conditions. However, there were fewer significant differences between mean durations for the individual months in the cool environmental chamber (LSD test; P<0.05; Figure 7b). In the warm chamber, the shortest mean ‘probe’ duration was in August and September compared with May, June, and July. October mean ‘probe’ duration doesn’t appear to fit trends suggesting it was a potential outlier. ‘Probe’ frequency was not correlated with the month of the bioassay in either environmental chamber (P>0.05). There were significant differences in total ‘probe’ duration per insect per day for months in the warm environmental chamber (F = 4.82; d.f. = 5, 84; P<0.001; Figure 7c), but not in the cool chamber (F = 1.30; d.f. = 5, 100; P = 0.272; Figure 7d). Similar to the ‘probe’ duration data discussed above, adult H. halys collected in May, June and July tended to display longer durations per day than in August and September (LSD test; P<0.05; Figure 7c).


Electronically monitored labial dabbing and stylet 'probing' behaviors of brown marmorated stink bug, Halyomorpha halys, in simulated environments.

Wiman NG, Walton VM, Shearer PW, Rondon SI - PLoS ONE (2014)

‘Probing’ duration and total ‘probing’ time for H. halys in each of warm and cool environmental chambers.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256200&req=5

pone-0113514-g007: ‘Probing’ duration and total ‘probing’ time for H. halys in each of warm and cool environmental chambers.
Mentions: The duration of ‘probes’ depended on the month in which the assay was conducted in both the warm (F = 8.11; d.f. = 5,84; P<0.001; Figure 7a), and the cool environmental chambers (F = 2.38; d.f. = 5,101; P = 0.043; Figure 7b). Some of the longest mean ‘probe’ durations occurred in chambers programmed to represent early season conditions. However, there were fewer significant differences between mean durations for the individual months in the cool environmental chamber (LSD test; P<0.05; Figure 7b). In the warm chamber, the shortest mean ‘probe’ duration was in August and September compared with May, June, and July. October mean ‘probe’ duration doesn’t appear to fit trends suggesting it was a potential outlier. ‘Probe’ frequency was not correlated with the month of the bioassay in either environmental chamber (P>0.05). There were significant differences in total ‘probe’ duration per insect per day for months in the warm environmental chamber (F = 4.82; d.f. = 5, 84; P<0.001; Figure 7c), but not in the cool chamber (F = 1.30; d.f. = 5, 100; P = 0.272; Figure 7d). Similar to the ‘probe’ duration data discussed above, adult H. halys collected in May, June and July tended to display longer durations per day than in August and September (LSD test; P<0.05; Figure 7c).

Bottom Line: We found that temperature had a significant impact on H. halys 'probing' behavior and may influence periodicity of activity.Our data suggest that the minimal temperature at which 'probing' of H. halys occurs is between 3.5 and 6.1 °C (95% CI), and that 'probing' does not occur at temperatures above 26.5 to 29.6 °C (95% CI).We estimated that the optimal temperature for 'probing' is between 16 and 17 °C.

View Article: PubMed Central - PubMed

Affiliation: Department of Horticulture, Oregon State University, Corvallis, Oregon, United States of America.

ABSTRACT
Brown marmorated stink bug, Halyomorpha halys (Stål), (Hemiptera: Pentatomidae) is an invasive polyphagous agricultural and urban nuisance pest of Asian origin that is becoming widespread in North America and Europe. Despite the economic importance of pentatomid pests worldwide, their feeding behavior is poorly understood. Electronically monitored insect feeding (EMIF) technology is a useful tool in studies of feeding behavior of Hemiptera. Here we examined H. halys feeding behavior using an EMIF system designed for high throughput studies in environmental chambers. Our objectives were to quantify feeding activity by monitoring proboscis contacts with green beans, including labial dabbing and stylet penetration of the beans, which we collectively define as 'probes'. We examined frequency and duration of 'probes' in field-collected H. halys over 48 hours and we determined how environmental conditions could affect diel and seasonal periodicity of 'probing' activity. We found differences in 'probing' activity between months when the assays were conducted. These differences in activity may have reflected different environmental conditions, and they also coincide with what is known about the phenology of H. halys. While a substantial number of 'probes' occurred during scotophase, including some of the longest mean 'probe' durations, activity was either lower or similar to 'probing' activity levels during photophase on average. We found that temperature had a significant impact on H. halys 'probing' behavior and may influence periodicity of activity. Our data suggest that the minimal temperature at which 'probing' of H. halys occurs is between 3.5 and 6.1 °C (95% CI), and that 'probing' does not occur at temperatures above 26.5 to 29.6 °C (95% CI). We estimated that the optimal temperature for 'probing' is between 16 and 17 °C.

Show MeSH
Related in: MedlinePlus