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First isolation of Leishmania from Northern Thailand: case report, identification as Leishmania martiniquensis and phylogenetic position within the Leishmania enriettii complex.

Pothirat T, Tantiworawit A, Chaiwarith R, Jariyapan N, Wannasan A, Siriyasatien P, Supparatpinyo K, Bates MD, Kwakye-Nuako G, Bates PA - PLoS Negl Trop Dis (2014)

Bottom Line: Here we report a case in a 52-year-old Thai male from northern Thailand, who presented with subacute fever, huge splenomegaly and pancytopenia.This is the first report of visceral leishmaniasis caused by L. martiniquensis from the region.Moreover, the majority of parasites previously identified as "L. siamensis" also appear to be L. martiniquensis.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.

ABSTRACT
Since 1996, there have been several case reports of autochthonous visceral leishmaniasis in Thailand. Here we report a case in a 52-year-old Thai male from northern Thailand, who presented with subacute fever, huge splenomegaly and pancytopenia. Bone marrow aspiration revealed numerous amastigotes within macrophages. Isolation of Leishmania LSCM1 into culture and DNA sequence analysis (ribosomal RNA ITS-1 and large subunit of RNA polymerase II) revealed the parasites to be members of the Leishmania enriettii complex, and apparently identical to L. martiniquensis previously reported from the Caribbean island of Martinique. This is the first report of visceral leishmaniasis caused by L. martiniquensis from the region. Moreover, the majority of parasites previously identified as "L. siamensis" also appear to be L. martiniquensis.

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Related in: MedlinePlus

Giemsa-stained promastigote forms from culture.A–H, examples showing morphological variation of forms observed, all at the same magnification, bar in F represents 5 µm. Procyclic-like promastigotes can be observed in A (arrows);, leptomonad-like promastigotes in B and C (arrows); nectomonad-like promastigotes in D, E and F; and rosettes and aggregates in G and H.
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pntd-0003339-g003: Giemsa-stained promastigote forms from culture.A–H, examples showing morphological variation of forms observed, all at the same magnification, bar in F represents 5 µm. Procyclic-like promastigotes can be observed in A (arrows);, leptomonad-like promastigotes in B and C (arrows); nectomonad-like promastigotes in D, E and F; and rosettes and aggregates in G and H.

Mentions: The Leishmania parasites from the BM specimens were cultured in Schneider's insect medium supplemented with 20% foetal bovine serum (FBS) at 25°C. Promastigote forms were first observed on day 3. Their morphology was generally similar to that described for other Leishmania species (Fig. 3). A range of promastigote morphologies was observed, including some similar to procyclic promastigotes, leptomonad promastigotes and nectomonad promastigotes (Fig. 3A–F) [19]. Although free swimming individual promastigotes were readily observed, rosettes and large aggregates of promastigotes were prevalent in culture (Fig. 3G, H). The strain was sub-cultured until neither red blood cells nor white blood cells were observed and then the promastigotes were cryopreserved in liquid nitrogen. These promastigotes have been continuously maintained in this medium for over one year, and have also been successfully transferred and grown in Medium 199 supplemented with 10% FBS. The WHO code for this strain is MHOM/TH/2012/LSCM1.


First isolation of Leishmania from Northern Thailand: case report, identification as Leishmania martiniquensis and phylogenetic position within the Leishmania enriettii complex.

Pothirat T, Tantiworawit A, Chaiwarith R, Jariyapan N, Wannasan A, Siriyasatien P, Supparatpinyo K, Bates MD, Kwakye-Nuako G, Bates PA - PLoS Negl Trop Dis (2014)

Giemsa-stained promastigote forms from culture.A–H, examples showing morphological variation of forms observed, all at the same magnification, bar in F represents 5 µm. Procyclic-like promastigotes can be observed in A (arrows);, leptomonad-like promastigotes in B and C (arrows); nectomonad-like promastigotes in D, E and F; and rosettes and aggregates in G and H.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4256172&req=5

pntd-0003339-g003: Giemsa-stained promastigote forms from culture.A–H, examples showing morphological variation of forms observed, all at the same magnification, bar in F represents 5 µm. Procyclic-like promastigotes can be observed in A (arrows);, leptomonad-like promastigotes in B and C (arrows); nectomonad-like promastigotes in D, E and F; and rosettes and aggregates in G and H.
Mentions: The Leishmania parasites from the BM specimens were cultured in Schneider's insect medium supplemented with 20% foetal bovine serum (FBS) at 25°C. Promastigote forms were first observed on day 3. Their morphology was generally similar to that described for other Leishmania species (Fig. 3). A range of promastigote morphologies was observed, including some similar to procyclic promastigotes, leptomonad promastigotes and nectomonad promastigotes (Fig. 3A–F) [19]. Although free swimming individual promastigotes were readily observed, rosettes and large aggregates of promastigotes were prevalent in culture (Fig. 3G, H). The strain was sub-cultured until neither red blood cells nor white blood cells were observed and then the promastigotes were cryopreserved in liquid nitrogen. These promastigotes have been continuously maintained in this medium for over one year, and have also been successfully transferred and grown in Medium 199 supplemented with 10% FBS. The WHO code for this strain is MHOM/TH/2012/LSCM1.

Bottom Line: Here we report a case in a 52-year-old Thai male from northern Thailand, who presented with subacute fever, huge splenomegaly and pancytopenia.This is the first report of visceral leishmaniasis caused by L. martiniquensis from the region.Moreover, the majority of parasites previously identified as "L. siamensis" also appear to be L. martiniquensis.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.

ABSTRACT
Since 1996, there have been several case reports of autochthonous visceral leishmaniasis in Thailand. Here we report a case in a 52-year-old Thai male from northern Thailand, who presented with subacute fever, huge splenomegaly and pancytopenia. Bone marrow aspiration revealed numerous amastigotes within macrophages. Isolation of Leishmania LSCM1 into culture and DNA sequence analysis (ribosomal RNA ITS-1 and large subunit of RNA polymerase II) revealed the parasites to be members of the Leishmania enriettii complex, and apparently identical to L. martiniquensis previously reported from the Caribbean island of Martinique. This is the first report of visceral leishmaniasis caused by L. martiniquensis from the region. Moreover, the majority of parasites previously identified as "L. siamensis" also appear to be L. martiniquensis.

Show MeSH
Related in: MedlinePlus